LOCUS       Exported                 688 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 
            promoter and sgRNA scaffold module for gRNA expression. Compatible 
            with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    pMuLE ENTR U6 stuffer sgRNA scaffold L1-R5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(39..162)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_RNA        complement(178..253)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(370..389)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tgcagtacag gtcactaata ccatctaagt agttgattca tagtgactgg atatgttgtg
       61 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
      121 tatcatttta cgtttctcgt tcaacttttg tatacaaagt tgtcgaattc aaaaaaagca
      181 ccgactcggt gccacttttt caagttgata acggactagc cttattttaa cttgctattt
      241 ctagctctaa aacgtcagca ggttcttgat ccgtcgagat tttcaggagc taaggaagct
      301 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
      361 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
      421 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
      481 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
      541 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
      601 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
      661 tattcgcaag atgtggcgtg ttacggtg
//
