LOCUS       Exported                1101 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  Retroviral expression of hTERT. Used to create immortalized cell 
            lines..
ACCESSION   .
VERSION     .
KEYWORDS    pBABE-puro-hTERT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Counter CM, Hahn WC, Wei W, Caddle SD, Beijersbergen RL, Lansdorp 
            PM, Sedivy JM, Weinberg RA
  TITLE     Dissociation among in vitro telomerase activity, telomere 
            maintenance, and cellular immortalization.
  JOURNAL   Proc Natl Acad Sci U S A 1998 Dec 8;95(25):14723-8.
  PUBMED    9843956
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             334..345
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 caggatggtc ttgaagtctg agggcagtgc cgggttggct gcggcctcca gggcagtcag
       61 cgtcgtcccc gggagcttcc gactcagctg cgtctgggct gtcctgagtg accccaggag
      121 tggcacgtag gtgacacggt gtcgagtcag cttgagcagg aatgcttggt ggcacagcca
      181 ctgcacggcc tcggagggca gagggccggc ggcgcccttg gcccccagcg acatccctgc
      241 gttcttggct ttcaggatgg agtagcagag ggaggccgtg tcagagatga cgcgcaggaa
      301 aaatgtgggg ttcttccaaa cttgctgatg aaatgggagc tgcagcacac atgcgtgaaa
      361 cctgtacgcc tgcagcagga ggatcttgta gatgttggtg cacaccgtct ggaggctgtt
      421 cacctgcaaa tccagaaaca ggctgtgaca cttcagccgc aagaccccaa agagtttgcg
      481 acgcatgttc ctcccagcct tgaagccgcg gttgaaggtg agactggctc tgatggaggt
      541 ccgggcatag ctggagtagt cgctctgcac ctccagggtc cgggtatcca gcagcaggcc
      601 gcaccagggg aataggccgt gggccggcat ctgaacaaaa gccgtgccac ccagggcctc
      661 gtcttctaca gggaagttca ccactgtctt ccgcaagttc accacgcagc catactcagg
      721 gacacctcgg accagggtcc tgaggaaggt tttcgcgtgg gtgaggtgag gtgtcaccaa
      781 caagaaatca tccaccaaac gcaggagcag cccgtcccgc cgaatccccg caaacagctt
      841 gttctccatg tcgccgtagc acaggctgca gagcagcgtg gagaggatgg agccctgcgg
      901 gatcccctgg cactggacgt aggacttgcc cctgatgcgc acggcgtggt ggcacatgaa
      961 gcgtaggaag acgtcgaaga ggccactgct ggcctcattc agggaggagc tctgctcgat
     1021 gacgacggca tccctcagcg ggctggtctc ctgcaggtga gccacgaact gtcgcatgta
     1081 cggctggagg tctgtcaagg t
//
