LOCUS Exported 568 bp ds-DNA linear SYN 30-MAY-2021 DEFINITION C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).. ACCESSION . VERSION . KEYWORDS PX854 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 568) AUTHORS Zetsche B, Volz SE, Zhang F TITLE A split-Cas9 architecture for inducible genome editing and transcription modulation. JOURNAL Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. PUBMED 25643054 REFERENCE 2 (bases 1 to 568) AUTHORS . TITLE Direct Submission JOURNAL Exported May 30, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..568 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 45..54 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 57..77 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 102..422 /codon_start=1 /product="human FK506-binding protein FKBP12" /label=FKBP /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL KLE" ORIGIN 1 ctggagcacc tgcctgaaat cacttttttt caggttggac cggtgccacc atggccccaa 61 agaagaagcg gaaggtcggt atccacggag tcccagcagc cggcgtgcag gtggagacca 121 tcagccccgg cgacggcaga accttcccca agagaggcca gacctgcgtg gtgcactaca 181 ccggcatgct ggaggacggc aagaagttcg acagcagcag agacagaaac aagcccttca 241 agttcatgct gggcaagcag gaggtgatca gaggctggga ggagggcgtg gcccagatga 301 gcgtgggcca gagagccaag ctgaccatca gccccgacta cgcctacggc gccaccggcc 361 accccggcat catccctcct cacgccaccc tggtgttcga cgtggagctg ctgaagctgg 421 agggtggcgg tggctcgggt ggcggtggct cgggtggcgg tggctcgtgc ttcgactccg 481 tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc 541 tgaaaattat caaggacaag gacttcct //