LOCUS       Exported                 948 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Localization: Mitochondria, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    tdTomato-Mito-7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Ai HW, Hazelwood KL, Davidson MW, Campbell RE
  TITLE     Fluorescent protein FRET pairs for ratiometric imaging of dual 
            biosensors.
  JOURNAL   Nat Methods. 2008 May . 5(5):401-3.
  PUBMED    18425137
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(4..78)
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     promoter        complement(107..310)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(136..156)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        311..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(869..888)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatcttggcg cgcggcactg ggagccgccg ggccgagcct gtcaagcccc gcagcagcag
       61 cggcgtcagg acggacatgc tagcggatct gacggttcac taaaccagct ctgcttatat
      121 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
      181 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
      241 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
      301 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
      361 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
      421 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
      481 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
      541 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
      601 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
      661 actattaata actaatgcat ggcggtaata cggttatcca cagaatcagg ggataacgca
      721 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      781 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      841 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      901 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtcc
//
