LOCUS       Exported                 677 bp ds-DNA     linear   SYN 21-MAY-2021
DEFINITION  a ligation-independent cloning (LIC) retroviral vector for stable 
            gene transduction in mammalian cells. Neo selection..
ACCESSION   .
VERSION     .
KEYWORDS    pBLIC-neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Patel A, Munoz A, Halvorsen K, Rai P
  TITLE     Creation and validation of a ligation-independent cloning (LIC) 
            retroviral vector for stable gene transduction in mammalian cells.
  JOURNAL   BMC Biotechnol. 2012 Jan 16;12:3. doi: 10.1186/1472-6750-12-3.
  PUBMED    22248071
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(313..642)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      327..462
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(381..400)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     627..647
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
       61 ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc
      121 agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt
      181 tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg
      241 gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag tgcttgcggc
      301 agcgtgaagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
      361 tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
      421 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg
      481 gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
      541 tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc
      601 tgctggggag cctggggact ttccacaccc taactgacac acattccaca gggtcgagct
      661 cacattccac gtgagat
//
