LOCUS       Exported                 678 bp ds-DNA     linear   SYN 21-MAY-2021
DEFINITION  a ligation-independent cloning (LIC) retroviral vector for stable 
            gene transduction in mammalian cells. Neo selection..
ACCESSION   .
VERSION     .
KEYWORDS    pBLIC-neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Patel A, Munoz A, Halvorsen K, Rai P
  TITLE     Creation and validation of a ligation-independent cloning (LIC) 
            retroviral vector for stable gene transduction in mammalian cells.
  JOURNAL   BMC Biotechnol. 2012 Jan 16;12:3. doi: 10.1186/1472-6750-12-3.
  PUBMED    22248071
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             238..654
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     584..606
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     622..638
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 agggactttg ggggccgttt ttgtggcccg acctgaggaa gggagtcgat gtggaatccg
       61 accccgtcag gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg
      121 tctgaatttt tgctttcggt ttggaaccga agccgcgcgt cttgtctgct gcagcgctgc
      181 agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaattagggc
      241 cagactgtta ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc
      301 gctcacaacc agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa
      361 tggccaacct ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc
      421 caggttaaga tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac
      481 atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac
      541 cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt
      601 tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg cgccggccgg
      661 atccgcacac catctcac
//
