LOCUS       Exported               16180 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Ai3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16180)
  AUTHORS   Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, 
            Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H
  TITLE     A robust and high-throughput Cre reporting and characterization 
            system for the whole mouse brain.
  JOURNAL   Nat Neurosci. 2010 Jan . 13(1):133-40.
  PUBMED    20023653
REFERENCE   2  (bases 1 to 16180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16180
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    3..1087
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     enhancer        1181..1484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1486..1763
                     /label=chicken beta-actin promoter
     intron          1765..2782
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2706..2728
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2790..2809
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2858..2891
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(2915..2948)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    3115..3249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3369..3503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3623..3757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3786..3819)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      3866..3875
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3872..4591
                     /codon_start=1
                     /product="enhanced yellow variant of GFP"
                     /label=Topaz YFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3917..3938)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4178..4197)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4525..4546
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4641..5229
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4694..4714)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5112..5123)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5232..5248)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5233..5249)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    5250..5457
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    5465..5498
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        5520..6019
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5555..5578)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    6039..6072
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      6073..6082
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6079..6882
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6142..6161)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6752..6771
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6892..7353
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     promoter        11735..12228
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             12255..12824
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphteria toxin Fragment A"
                     /label=DTA
                     /translation="DDVVILLIFCMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDD
                     DWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELG
                     LSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEIN
                     FETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          12901..12966
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    13040..13264
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(13332..13352)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13332..13350)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(13371..13387)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13371..13387)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13384..13406)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13395..13411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(13419..13449)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13464..13485
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13602..13619)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13773..14361)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13853..13872)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14532..15392)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15155..15174
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15393..15497)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(15523..15978)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15660..15681)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15872..15891
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16105..16127
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     16119..16136
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     16120..16136
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16146..16165
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16146..16164
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggancgctt ccggccgacg tctcgtcgct gattggcttn ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgn acgtgcgggc gcggtcggcc tctggcgggg cgggggaggg
      421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttgcccc ctcttcccct cgtgatctgc aactccagtc
     1081 tttctagtta attaaccgtt taaacaattc tgcaggaatc tagttattaa tagtaatcaa
     1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1441 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     1501 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     1561 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
     1621 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
     1681 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     1741 aaaagcgaag cgcgcggcgg gcggggagtc gctgcgacgc tgccttcgcc ccgtgccccg
     1801 ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt
     1861 gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct
     1921 tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg
     1981 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc
     2041 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag
     2101 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg
     2161 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc
     2221 ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc
     2281 gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg
     2341 gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg
     2401 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
     2461 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc
     2521 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc
     2581 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
     2641 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag
     2701 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca
     2761 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     2821 ttttggcaaa gaattgattt gataccgcgg gccctaagaa gttcctattc tctagaaagt
     2881 ataggaactt cgtcgacatt taaatcattt aaatataact tcgtataatg tatgctatac
     2941 gaagttattc gcgatgaata aatgaaagct tgcagatctg cgactctaga ggatctgcga
     3001 ctctagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     3061 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     3121 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3181 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3241 gtctggatct gcgactctag aggatcataa tcagccatac cacatttgta gaggttttac
     3301 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     3361 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3421 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3481 atgtatctta tcatgtctgg atctgcgact ctagaggatc ataatcagcc ataccacatt
     3541 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     3601 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     3661 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3721 gtccaaactc atcaatgtat cttatcatgt ctggatcccc atcaagctga tccggaaccc
     3781 ttaatataac ttcgtataat gtatgctata cgaagttatt aggtccctcg acctgcagcc
     3841 caagctagat cgaattcggc cggccgccac catggtgagc aagggcgagg agctgttcac
     3901 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     3961 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     4021 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcgtgca
     4081 gtgcttcgcc cgctaccccg accacatgcg ccagcacgac ttcttcaagt ccgccatgcc
     4141 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     4201 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     4261 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     4321 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     4381 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     4441 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     4501 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4561 cactctcggc atggacgagc tgtacaagta acctaggtag agggccggcc ctgcaggaat
     4621 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     4681 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4741 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4801 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4861 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4921 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4981 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5041 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5101 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5161 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5221 cctccccgca tcgataccgt cgacctcgac tgtgccttct agttgccagc catctgttgt
     5281 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     5341 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     5401 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggaact
     5461 agtggtgcca gggcgtgccc ttgggctccc cgggcgcggc ggccgcatcg aattctaccg
     5521 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     5581 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     5641 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     5701 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     5761 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     5821 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     5881 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     5941 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     6001 ctcatctccg ggcctttcga cctgcaatcg ccgctagcga agttcctatt ctctagaaag
     6061 tataggaact tcgccaccat gggatcggcc attgaacaag atggattgca cgcaggttct
     6121 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     6181 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     6241 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     6301 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     6361 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     6421 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     6481 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     6541 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     6601 gccaggctca aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc
     6661 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     6721 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     6781 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     6841 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaggggatcc gctgtaagtc
     6901 tgcagaaatt gatgatctat taaacaataa agatgtccac taaaatggaa gtttttcctg
     6961 tcatactttg ttaagaaggg tgagaacaga gtacctacat tttgaatgga aggattggag
     7021 ctacgggggt gggggtgggg tgggattaga taaatgcctg ctctttactg aaggctcttt
     7081 actattgctt tatgataatg tttcatagtt ggatatcata atttaaacaa gcaaaaccaa
     7141 attaagggcc agctcattcc tcccactcat gatctataga tctatagatc tctcgtggga
     7201 tcattgtttt tctcttgatt cccactttgt ggttctaagt actgtggttt ccaaatgtgt
     7261 cagtttcata gcctgaagaa cgagatcagc agcctctgtt ccacatacac ttcattctca
     7321 gtattgtttt gccaagttct aattccatca gaaagcttgc agaagatctc cccaactggg
     7381 gtaacctttg agttctctca gttggggggg cgcgccctag aagatgggcg ggagtcttct
     7441 gggcaggctt aaaggctaac ctggtgtgtg ggcgttgtcc tgcaggggaa ttgaacaggt
     7501 gtaaaattgg agggacaaga cttcccacag attttcggtt ttgtcgggaa gttttttaat
     7561 aggggcaaat aggaaaatgg aggataggag tcatctgggg tttatgcagc aaaactacag
     7621 gtatattgct tgtatccgcc tcggagattt ccatgaggag ataaagacat gtcacccgag
     7681 tttatactct cctgcttaga tcctactaca gtatgaaata cagtgtngcg aggtagacta
     7741 tgtaagcaga tttaatcatt ttaaagagcc cagtacttca tatccatttc tcccgctcct
     7801 tctgcagcct tatcaaaagg tatttagaac actcatttta gccccatttt catttattat
     7861 actggcttat ccaaccccta gacagagcat tggcattttc cctttcctga tcttagaagt
     7921 ctgatgactc atgaaaccag acagattagt tacatacacc acaaatcgag gctgtagctg
     7981 gggcctcaac actgcagttc ttttataact ccttagtaca ctttttgttg atcctttgcc
     8041 ttgatcctta attttcagtg tctatcacct ctcccgtcag gtggtgttcc acatttgggc
     8101 ctattctcag tccagggagt tttacaacaa tagatgtatt gagaatccaa cctaaagctt
     8161 aactttccac tcccatgaat gcctctctcc tttttctcca ttataactga gctatnacca
     8221 ttaatggttt caggtggatg tctcctcccc caatatacct gatgtatcta catattgcca
     8281 ggctgatatt ttaagacatn aaaggtatat ttcattattg agccacatgg tattgattac
     8341 tgctactaaa attttgtcat tgtacacatc tgtaaaaggt ggttcctttt ggaatgcaaa
     8401 gttcaggtgt ttgttgtctt tcctgaccta aggtcttgtg agcttgtatt ttttctattt
     8461 aagcagtgct ttctcttgga ctggcttgac tcatggcatt ctacacgtta ttgctggtct
     8521 aaatgtgatt ttgccaagct tcttcaggac ctataatttt gcttgacttg tagccaaaca
     8581 caagtaaaat gattaagcaa caaatgtatt tgtgaagctt ggtttttagg ttgttgtgtt
     8641 gtgtgtgctt gtgctctata ataatactat ccaggggctg gagaggtggc tcggagttca
     8701 agagcacaga ctgctcttcc agaagtcctg agttcaattc ccagcaacca catggtggct
     8761 cacaaccatc tgtaatggga tctgatgccc tcttctggtg tgtctgaaga ccacaagtgt
     8821 attcacatta aataaataat cctccttctt cttctttttt tttttttaaa gagaatnctg
     8881 tctccagtag aattactgaa gtaatgaaat actttgtgtt tgttccaata tggnagccaa
     8941 taatcaaata ctcttnagca ctggaaatgt accaaggaac tattttattt aagtgnactg
     9001 tggacagagg agccataact gcagacttgt gggatacaga agaccaatgc agacttaatg
     9061 tcttttctct tacactaagc aataaagaaa taaaaattga acttctagta tcctatttgt
     9121 taaactgcta gctttactaa cttttgtgct tcatctatac aaagctgaaa gctaagtctg
     9181 cagccattac taaacatgaa agcaagtaat gataattttg gatttcaaaa atgtagggcc
     9241 agagtttagc cagccagtgg tggtgcttgc ctttatgcct taatcccagc actctggagg
     9301 cagagacagg cagatctctg agtttgagcc cagcctggtc tacacatcaa gttctatcta
     9361 ggatagccag gaatacacac agaaaccctg ttggggaggg gggctctgag atttcataaa
     9421 attataattg aagcattccc taatgagcca ctatggatgt ggctaaatcc gtctaccttt
     9481 ctgatgagat ttgggtatta ttttttctgt ctctgctgtt ggttgggtct tttgacactg
     9541 tgggctttct taaagcctcc ttccctgcca tgtggtctct tgtttgctac taacttccca
     9601 tggcttaaat ggcatggctt tttgccttct aagggcagct gctgagnttt gcagcctgat
     9661 ttccagggtg gggttgggaa atctttcaaa cactaaaatt gtcctttaat ttttttttaa
     9721 aaaatgggtt atataataaa cctcataaaa tagttatgag gagtgaggtg gactaatatt
     9781 aatgagtccc tcccctataa aagagctatt aaggcttttt gtcttatact aacttttttt
     9841 ttaaatgtgg tatctttaga accaagggtc ttagagtttt agtatacaga aactgttgca
     9901 tcgcttaatc agattttcta gtttcaaatc cagagaatcc aaattcttca cagccaaagt
     9961 caaattaaga atttctgact ttaatgttat ttgctactgt gaatataaaa tgatagcttt
    10021 tcctgaggca gggtctcact atgtatctct gcctgatctg caacaagata tgtagactaa
    10081 agttctgcct gcttttgtct cctgaatact aaggttaaaa tgtagtaata cttttggaac
    10141 ttgcaggtca gattctttta taggggacac actaagggag cttgggtgat agttggtaaa
    10201 tgtgtttaag tgatgaaaac ttgaattatt atcaccgcaa cctacttttt aaaaaaaaaa
    10261 gccaggcctg ttagagcatg ctaagggatc cctaggactt gctgagcaca caagagtagt
    10321 acttggcagg ctcctggtga gagcatattt caaaaaacaa ggcagacaac caagaaacta
    10381 cagtaaggtt acctgtcttt aaccatctgc atatacacag ggatattaaa atattccaaa
    10441 taatatttca ttcaagtttt cccccatcaa attgggacat ggatttctcc ggtgaatagg
    10501 cagagttgga aactaaacaa atgttggttt tgtgatttgt gaaattgttt tcaagtgata
    10561 gttaaagccc atgagataca gaacaaagct gctatttcga ggtctcttgg ttatactcag
    10621 aagcacttct ttgggtttcc ctgcactatc ctgatcatgt gctaggcctn ccttaggctg
    10681 attgttgttc aaataactta agtttcctgt caggtgatgt catatgattt catatatcaa
    10741 ggcaaaacat gttatatatg ttaaacattt gnacttaatg tgaaagttag gtctttgtgg
    10801 gttttgattt taatttcaaa acctgagcta aataagtcat tttacatgtc ttacatttgg
    10861 tgaattgtat attgtggttt gcaggcaaga ctctctgacc tagtaaccct cctatagagc
    10921 actttgctgg gtcacaagtc taggagtcaa gcatttcacc ttgaagttga gacgttttgt
    10981 tagtgtatac tagttatatg ttggaggaca tgtttatcca gaagatattc aggactattt
    11041 ttgactgggc taaggaattg attctgatta gcactgttag tgagcattga gtggccttta
    11101 ggcttgaatt ggagtcactt gtatatctca aataatgctg gccttttttn aaaagccctt
    11161 gttctttatc accctgtttt ctacataatt tttgttcaaa gaaatacttg tttggatctc
    11221 cttttgacaa caatagcatg ttttcaagcc atattttttt tccttttttt tttttttttt
    11281 ggtttttcga gacagggttt ctctgtatag ccctggctgt cctggaactc actttgtaga
    11341 ccaggctggc ctcgaactca gaaatccgcc tgcctctgcc tcctgagtgc cgggattaaa
    11401 ggcgtgcacc accacgcctg gctaagttgg atattttgta tataactata accaatacta
    11461 actccactgg gtggattttt aattcagtca gtagtcttaa gtggtcttta ttggccctta
    11521 ttaaaatcta ctgttcactc taacagaggc tgttggacta gtggnactaa gcaacttcct
    11581 acggatatac tagcagataa gggtcaggga tagaaactag tctagcgttt tgtataccta
    11641 ccagcttata ctaccttgtt ctgatagaaa tatttaggac atctagctta tcgatccgtc
    11701 gacggtatcg ataagcttga tatcgaattc taccgggtag gggaggcgct tttccaaggc
    11761 agtctgagca tgcgcttagc agccccgctg gcacttggcg ctacacaagt ggcctntggc
    11821 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
    11881 tcgcgccacc ttctnctcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
    11941 cgtnaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtcagat ggacagcacc
    12001 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
    12061 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
    12121 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
    12181 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcaggtcc
    12241 tcgccatgga tcctgatgat gttgttattc ttctaatctt ttgtatggaa aacttttctt
    12301 cgtaccacgg gactaaacct ggttatgtag attccattca aaaaggtata caaaagccaa
    12361 aatctggtac acaaggaaat tatgacgatg attggaaagg gttttatagt accgacaata
    12421 aatacgacgc tgcgggatac tctgtagata atgaaaaccc gctctctgga aaagctggag
    12481 gcgtggtcaa agtgacgtat ccaggactga cgaaggttct cgcactaaaa gtggataatg
    12541 ccgaaactat taagaaagag ttaggtttaa gtctcactga accgttgatg gagcaagtcg
    12601 gaacggaaga gtttatcaaa aggttcggtg atggtgcttc gcgtgtagtg ctcagccttc
    12661 ccttcgctga ggggagttct agcgttgaat atattaataa ctgggaacag gcgaaagcgt
    12721 taagcgtaga acttgagatt aattttgaaa cccgtggaaa acgtggccaa gatgcgatgt
    12781 atgagtatat ggctcaagcc tgtgcaggaa atcgtgtcag gcgatctctt tgtgaaggaa
    12841 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
    12901 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
    12961 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc agatcctaga
    13021 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
    13081 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
    13141 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
    13201 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
    13261 atggcttctg aggcggaaag aaccagctgg ggctcgacct cgaggggggg cccggtaccc
    13321 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
    13381 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    13441 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    13501 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    13561 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    13621 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    13681 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    13741 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    13801 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    13861 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    13921 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    13981 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    14041 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    14101 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    14161 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
    14221 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    14281 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    14341 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    14401 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    14461 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    14521 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    14581 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    14641 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    14701 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    14761 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    14821 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    14881 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    14941 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    15001 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    15061 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    15121 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    15181 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    15241 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    15301 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    15361 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    15421 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    15481 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
    15541 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
    15601 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
    15661 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
    15721 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
    15781 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
    15841 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
    15901 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
    15961 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    16021 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    16081 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
    16141 gcgcgtaata cgactcacta tagggcgaat tggagctcca
//
