LOCUS Exported 2996 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBTL-4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2996) AUTHORS Lynch MD, Gill RT TITLE Broad host range vectors for stable genomic library construction. JOURNAL Biotechnol Bioeng. 2006 May 5. 94(1):151-8. PUBMED 16496398 REFERENCE 2 (bases 1 to 2996) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2996 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 60..96 /label=soxR terminator /note="bidirectional E. coli soxR transcription terminator" rep_origin 102..871 /label=pBBR1 oriV /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication" CDS 872..1534 /codon_start=1 /product="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" /label=pBBR1 Rep /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR" CDS 1631..2821 /codon_start=1 /gene="tet" /product="tetracycline efflux protein" /label=TcR /note="confers resistance to tetracycline" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST" primer_bind complement(1983..2002) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" terminator 2841..2872 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 2919..2949 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2957..2973 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." ORIGIN 1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga 61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg 121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca 181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc 241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag 301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc 361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc 421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg 481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt 541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg 601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg 661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac 721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg 781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa 841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat 901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg 961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca 1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg 1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt 1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg 1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca 1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta 1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat 1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg 1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga 1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca 1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa 1621 ggaagctaaa atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga 1681 tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca 1741 ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt 1801 tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct 1861 cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg 1921 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg 1981 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag 2041 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat 2101 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg 2161 ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt 2221 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac 2281 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg 2341 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat 2401 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa 2461 gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc 2521 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat 2581 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca 2641 aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc 2701 gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct 2761 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg 2821 aatggaaggg atccccctca agtcaaaagc ctccggtcgg aggcttttga ctttctgcta 2881 tggaggtcag gtatgatttt gcattaggca ccccaggctt tacactttat gcttccggct 2941 cgtatgttgt gtggaattgt gagcggataa caatttcaca ctctagagaa ttcgtc //