LOCUS Exported 4000 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS).. ACCESSION . VERSION . KEYWORDS 5'UTR CGG 99x FMR1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4000) TITLE Charlet-Berguerand plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 4000) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4000 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(1..105) /gene="bla" /label=AmpR promoter primer_bind 173..191 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" enhancer 355..734 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 735..938 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 889..909 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 983..1002 /label=T7 /note="T7 promoter, forward primer" promoter 983..1001 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 1684..1805 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1695..1714) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1749..1768 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(1949..1971) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 2055..2195 /label=bom /note="basis of mobility region from pBR322" primer_bind 2110..2129 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(2210..2227) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2381..2969) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2461..2480) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3140..4000) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3763..3782 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 121 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 181 tatcacgagg ccctttcgtc ttcaagaatt cagatcttca atattggcca ttagccatat 241 tattcattgg ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc 301 tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca tgttggcatt 361 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 421 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 481 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 541 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 601 atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 661 atgcccagta catgacctta cgggactttc ctacttggca gtacatctac gtattagtca 721 tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg 781 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 841 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 901 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 961 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 1021 gtttaaactt aagcttcgtc gacagggccc accgcggagg ccggcctcag tcaggcgctc 1081 agctccgttt cggtttcact tccggtggag ggccgcctct gagcgggcgg cgggccgacg 1141 gcgagcgcgg gcggcggcgg tgacggaggc gccgctgcca gggggcgtgc ggcagcgcgg 1201 cggcggcggc ggcggcggcg gcggcggcgg cggcggcgga ggcggcggcg gcggcggcgg 1261 cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg 1321 cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg 1381 cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg 1441 cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcggctgggc 1501 ctcgagcgcc cgcagcccac ctctcggggg cgggctcccg gcgctagcag ggctgaagag 1561 aagatggagg agctggtggt ggaagtgcgg ggctccaatg gcgctttcta caaggcacct 1621 aggctaggcg cgccgatatc aggcctcctg aggatctaga gcggccgctt cgagcagaca 1681 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct 1741 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac 1801 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg 1861 ttttttaaag caagtaaaac ctctacaaat gtggtaacag ctgcacgtgg gatccctgcc 1921 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 1981 cagcttgtct gtaagcggat gccgagcaga caagcccgtc agggcgcgtc agcgggtgtt 2041 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc 2101 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 2161 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg 2221 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 2281 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 2341 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 2401 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 2461 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 2521 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 2581 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 2641 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 2701 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 2761 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 2821 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaan agagttggta 2881 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 2941 agattacgcg cagaanaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 3001 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 3061 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 3121 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 3181 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 3241 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 3301 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 3361 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 3421 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt 3481 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 3541 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 3601 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 3661 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 3721 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata 3781 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 3841 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 3901 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 3961 aaaagggaat aagggcgaca cggaaatgtt gaatactcat //