LOCUS Exported 3586 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBTB-3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3586) AUTHORS Lynch MD, Gill RT TITLE Broad host range vectors for stable genomic library construction. JOURNAL Biotechnol Bioeng. 2006 May 5. 94(1):151-8. PUBMED 16496398 REFERENCE 2 (bases 1 to 3586) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3586 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 60..96 /label=soxR terminator /note="bidirectional E. coli soxR transcription terminator" rep_origin 102..871 /label=pBBR1 oriV /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication" CDS 872..1534 /codon_start=1 /product="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" /label=pBBR1 Rep /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR" CDS 1631..2290 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1697..1716) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" terminator 2309..2340 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" CDS complement(2368..3246) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" promoter 3273..3557 /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind 3480..3499 /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" ORIGIN 1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga 61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg 121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca 181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc 241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag 301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc 361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc 421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg 481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt 541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg 601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg 661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac 721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg 781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa 841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat 901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg 961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca 1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg 1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt 1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg 1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca 1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta 1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat 1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg 1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga 1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca 1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa 1621 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca 1681 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt 1741 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc 1801 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat 1861 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga 1921 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct 1981 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg 2041 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga 2101 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta 2161 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga 2221 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg 2281 cggggcgtaa acgcgtggat ccccctcaag tcaaaagcct ccggtcggag gcttttgact 2341 ttctgctatg gaggtcaggt atgattttta tgacaacttg acggctacat cattcacttt 2401 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg 2461 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg 2521 ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac 2581 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg 2641 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca 2701 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg 2761 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc 2821 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc 2881 cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta 2941 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc 3001 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc 3061 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt 3121 cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa 3181 acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc 3241 agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag 3301 acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct 3361 tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag 3421 tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta 3481 tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact 3541 ctctactgtt tctccatacc cgtttttttg ggctagagaa ttcgtc //