LOCUS Exported 783 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance. ACCESSION . VERSION . KEYWORDS pBUN421 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 783) AUTHORS Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ TITLE A CRISPR/Cas9 toolkit for multiplex genome editing in plants. JOURNAL BMC Plant Biol. 2014 Nov 29;14(1):327. PUBMED 25432517 REFERENCE 2 (bases 1 to 783) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..783 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(310..330) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(337..402) /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" ORIGIN 1 agaatcgtca accttcgcca tctcgttgga gaaaatctcc tgcaggtagc agatgcgatt 61 cttcctgcgc gtgtaccgcc tgcgggcggt gcgcttgagc cgcgtagcct cagccgtctc 121 gccgctgtcg aacaggagag cgccaatgag attcttcttg atggaatgcc gatcggtgtt 181 gcccaggacc ttgaacttct ttgagggcac cttgtactcg tcggtgatca cggcccagcc 241 aacagagtta gtcccaatat cgaggccgat cgagtacttc ttgtcagcag ctggcacccc 301 gtggatgcca accttcctct tcttcttcgg agccatcttg tcatcatcat ccttgtaatc 361 aatgtcgtgg tccttgtaat ccccgtcgtg gtccttgtaa tccatctaga gcggccgcgg 421 ccatacaagt attggggatc ccccgggctg cagaagtaac accaaacaac agggtgagca 481 tcgacaaaag aaacagtacc aagcaaataa atagcgtatg aaggcagggc taaaaaaatc 541 cacatatagc tgctgcatat gccatcatcc aagtatatca agatcaaaat aattataaaa 601 catacttgtt tattataata gataggtact caaggttaga gcatatgaat agatgctgca 661 tatgccatca tgtatatgca tcagtaaaac ccacatcaac atgtatacct atcctagatc 721 gatatttcca tccatcttaa actcgtaact atgaagatgt atgacacaca catacagttc 781 caa //