LOCUS Exported 4831 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBMTB-3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4831) AUTHORS Lynch MD, Gill RT TITLE Broad host range vectors for stable genomic library construction. JOURNAL Biotechnol Bioeng. 2006 May 5. 94(1):151-8. PUBMED 16496398 REFERENCE 2 (bases 1 to 4831) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4831 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 60..96 /label=soxR terminator /note="bidirectional E. coli soxR transcription terminator" rep_origin 1347..2116 /label=pBBR1 oriV /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication" CDS 2117..2779 /codon_start=1 /product="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" /label=pBBR1 Rep /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR" CDS 2876..3535 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(2942..2961) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" terminator 3554..3585 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" CDS complement(3613..4491) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" promoter 4518..4802 /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind 4725..4744 /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" ORIGIN 1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga 61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt 121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct 181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct 241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa 301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc 361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt 421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt 481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt 541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct 601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct 661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct 721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg 781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga 841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt 901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc 961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt 1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca 1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt 1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg 1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc 1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc 1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt 1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag 1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc 1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc 1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg 1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc 1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt 1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc 1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg 1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa 1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc 1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt 2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta 2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa 2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc 2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa 2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt 2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg 2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga 2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga 2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca 2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc 2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc 2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt 2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc 2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga 2881 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt 2941 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac 3001 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat 3061 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct 3121 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt 3181 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca 3241 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat 3301 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa 3361 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa 3421 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg 3481 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg 3541 tggatccccc tcaagtcaaa agcctccggt cggaggcttt tgactttctg ctatggaggt 3601 caggtatgat ttttatgaca acttgacggc tacatcattc actttttctt cacaaccggc 3661 acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg 3721 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag 3781 cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg 3841 ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc 3901 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg 3961 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg 4021 ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat 4081 gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc 4141 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa 4201 gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc 4261 tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt 4321 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg 4381 gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg 4441 ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat 4501 actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg 4561 cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct 4621 gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg 4681 cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt 4741 atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc 4801 atacccgttt ttttgggcta gagaattcgt c //