LOCUS Exported 6979 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAAV-mCAMK-RFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6979) AUTHORS Nathanson JL, Yanagawa Y, Obata K, Callaway EM TITLE Preferential labeling of inhibitory and excitatory cortical neurons by endogenous tropism of adeno-associated virus and lentivirus vectors. JOURNAL Neuroscience. 2009 Jun 30. 161(2):441-50. PUBMED 19318117 REFERENCE 2 (bases 1 to 6979) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6979 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 7..136 /label=AAV2 ITR promoter 234..1523 /label=CaMKII promoter /note="promoter for the mouse calcium/calmodulin-dependent kinase II alpha subunit" intron 1587..2062 /label=-beta-globin intron /note="internally truncated intron from human beta-globin" primer_bind 2144..2160 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 2144..2160 /label=pBluescriptSK /note="For pBluescript vector" regulatory 2192..2201 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2198..2875 /codon_start=1 /product="rapidly maturing tetrameric variant of DsRed fluorescent protein (Bevis and Glick, 2002)" /label=DsRed-Express /note="mammalian codon-optimized" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH LFL" primer_bind complement(2328..2346) /label=mCherry-R /note="mCherry, reverse primer" primer_bind complement(2378..2398) /label=DsRed1-N /note="DsRed1, reverse primer" primer_bind 2596..2615 /label=mCherry-F /note="mCherry, forward primer" primer_bind 2790..2813 /label=DsRed1-C /note="DsRed1, forward primer" promoter complement(2918..2936) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(2919..2936) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(2956..2973) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2962..3073 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 3764..4277 /direction=RIGHT /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3851..3870) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4061..4082 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4758..4777) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4877..4899 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4937..4955) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 5023..5127 /gene="bla" /label=AmpR promoter CDS 5128..5988 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5346..5365) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6159..6747 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6648..6667 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6901..6918 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg 61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc 121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca 181 tgctctagag gatccttcga aaagcttgta tacggccggc cacgcgttcg taacattatg 241 gccttaggtc acttcatctc catggggttc ttcttctgat tttctagaaa atgagatggg 301 ggtgcagaga gcttcctcag tgacctgccc agggtcacat cagaaatgtc agagctagaa 361 cttgaactca gattactaat cttaaattcc atgccttggg ggcatgcaag tacgatatac 421 agaaggagtg aactcattag ggcagatgac caatgagttt aggaaagaag agtccagggc 481 agggtacatc tacaccaccc gcccagccct gggtgagtcc agccacgttc acctcattat 541 agttgcctct ctccagtcct accttgacgg gaagcacaag cagaaactgg gacaggagcc 601 ccaggagacc aaatcttcat ggtccctctg ggaggatggg tggggagagc tgtggcagag 661 gcctcaggag gggccctgct gctcagtggt gacagatagg ggtgagaaag cagacagagt 721 cattccgtca gcattctggg tctgtttggt acttcttctc acgctaaggt ggcggtgtga 781 tatgcacaat ggctaaaaag cagggagagc tggaaagaaa caaggacaga gacagaggcc 841 aagtcaacca gaccaattcc cagaggaagc aaagaaacca ttacagagac tacaaggggg 901 aagggaagga gagatgaatt agcttcccct gtaaacctta gaacccagct gttgccaggg 961 caacggggca atacctgtct cttcagagga gatgaagttg ccagggtaac tacatcctgt 1021 ctttctcaag gaccatccca gaatgtggca cccactagcc gttaccatag caactgcctc 1081 tttgccccac ttaatcccat cccgtctgtt aaaagggccc tatagttgga ggtgggggag 1141 gtaggaagag cgatgatcac ttgtggacta agtttgttca catccccttc tccaaccccc 1201 tcagtacatc accctgggag aacaaggtcc acttgcttct gggcccacac agtcctgcag 1261 tattgtgtat ataaggccag ggcaacggag gagcaggttt tgaagtgaaa ggcaggcagg 1321 tgttggggag gcagttaccg gggcaacggg aacagggcgt ttcggaggtg gttgccatgg 1381 ggacctggat gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa 1441 gccccaagct cgtcaatcaa gctggttctc catttgcact caggagcacg ggcaggcgag 1501 tggcccctag ttctgggggc agctctagag cgatcccccg accgcgggct agcggcgcgc 1561 cgagctcgga tcctgagaac ttcagggtga gtctatggga cccttgatgt tttctttccc 1621 cttcttttct atggttaagt tcatgtcata ggaaggggag aagtaacagg gtacacatat 1681 tgaccaaatc agggtaattt tgcatttgta attttaaaaa atgctttctt cttttaatat 1741 acttttttgt ttatcttatt tctaatactt tccctaatct ctttctttca gggcaataat 1801 gatacaatgt atcatgcctc tttgcaccat tctaaagaat aacagtgata atttctgggt 1861 taaggcaata gcaatatttc tgcatataaa tatttctgca tataaattgt aactgatgta 1921 agaggtttca tattgctaat agcagctaca atccagctac cattctgctt ttattttatg 1981 gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa tcatgttcat 2041 acctcttatc ttcctcccac agctcctggg caacgtgctg gtctgtgtgc tggcccatca 2101 ctttggcaaa gcacgtgaat tcgaagatct ggccgcctgg gcctctagaa ctagtggatc 2161 ccccgggctg caggaattcg atgctaccgt cgccaccatg gcctcctccg aggacgtcat 2221 caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga 2281 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt 2341 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tccagtacgg 2401 ctccaaggtg tacgtgaagc accccgccga catccccgac tacaagaagc tgtccttccc 2461 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac 2521 ccaggactcc tccctgcagg acggctcctt catctacaag gtgaagttca tcggcgtgaa 2581 cttcccctcc gacggccccg taatgcagaa gaagactatg ggctgggagg cctccaccga 2641 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc cacaaggccc tgaagctgaa 2701 ggacggcggc cactacctgg tggagttcaa gtccatctac atggccaaga agcccgtgca 2761 gctgcccggc tactactacg tggactccaa gctggacatc acctcccaca acgaggacta 2821 caccatcgtg gagcagtacg agcgcgccga gggccgccac cacctgttcc tgtagacggc 2881 caaatgaatt cccaaaccgt acgggaaacg gccctattct atagtgtcac ctaaatgcta 2941 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 3001 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 3061 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 3121 aggacagcaa gggggaggat tgggaagaca atagcgcggc cgctctagag catggctacg 3181 tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg 3241 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac 3301 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgccagctg gcgtaatagc 3361 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaat 3421 tccagacgat tgagcgtcaa aatgtaggta tttccatgag cgtttttcct gttgcaatgg 3481 ctggcggtaa tattgttctg gatattacca gcaaggccga tagtttgagt tcttctactc 3541 aggcaagtga tgttattact aatcaaagaa gtattgcgac aacggttaat ttgcgtgatg 3601 gacagactct tttactcggt ggcctcactg attataaaaa cacttctcag gattctggcg 3661 taccgttcct gtctaaaatc cctttaatcg gcctcctgtt tagctcccgc tctgattcta 3721 acgaggaaag cacgttatac gtgctcgtca aagcaaccat agtacgcgcc ctgtagcggc 3781 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 3841 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 3901 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 3961 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 4021 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 4081 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 4141 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 4201 atattaacgt ttacaattta aatatttgct tatacaatct tcctgttttt ggggcttttc 4261 tgattatcaa ccggggtaca tatgattgac atgctagttt tacgattacc gttcatcgat 4321 tctcttgttt gctccagact ctcaggcaat gacctgatag cctttgtaga gacctctcaa 4381 aaatagctac cctctccggc atgaatttat cagctagaac ggttgaatat catattgatg 4441 gtgatttgac tgtctccggc ctttctcacc cgtttgaatc tttacctaca cattactcag 4501 gcattgcatt taaaatatat gagggttcta aaaattttta tccttgcgtt gaaataaagg 4561 cttctcccgc aaaagtatta cagggtcata atgtttttgg tacaaccgat ttagctttat 4621 gctctgaggc tttattgctt aattttgcta attctttgcc ttgcctgtat gatttattgg 4681 atgttggaat tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc 4741 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac 4801 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga 4861 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa 4921 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata 4981 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 5041 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 5101 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 5161 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 5221 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 5281 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 5341 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 5401 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 5461 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 5521 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 5581 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 5641 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 5701 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 5761 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 5821 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 5881 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 5941 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 6001 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 6061 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 6121 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 6181 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 6241 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 6301 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 6361 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 6421 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 6481 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 6541 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 6601 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 6661 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 6721 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 6781 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 6841 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 6901 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 6961 cgttggccga ttcattaat //