LOCUS Exported 8298 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAAV-fSST-RFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8298) AUTHORS Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S, Callaway EM TITLE Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types. JOURNAL Front Neural Circuits. 2009 . 3():19. PUBMED 19949461 REFERENCE 2 (bases 1 to 8298) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8298 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 7..136 /label=AAV2 ITR CDS 2638..2667 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" CDS 2641..2670 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" intron 2865..3339 /label=-beta-globin intron /note="internally truncated intron from human beta-globin" misc_feature 3442..3494 /label=MCS /note="multiple cloning site" regulatory 3502..3511 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3508..4185 /codon_start=1 /product="improved tetrameric variant of DsRed fluorescent protein" /label=DsRed2 /note="mammalian codon-optimized" /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH LFL" primer_bind complement(3688..3708) /label=DsRed1-N /note="DsRed1, reverse primer" primer_bind 4100..4123 /label=DsRed1-C /note="DsRed1, forward primer" promoter complement(4236..4254) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(4237..4254) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(4274..4291) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4280..4391 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5082..5595 /direction=RIGHT /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5169..5188) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5379..5400 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6076..6095) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6195..6217 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(6255..6273) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6341..6445 /gene="bla" /label=AmpR promoter CDS 6446..7306 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6664..6683) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7477..8065 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7966..7985 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8219..8236 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg 61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc 121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca 181 tgctctagag gatccttcga aaagcttgta tacggccggc cacgcgtagc tgatgctcag 241 caggaatcca gatgattccc tctgagcgaa gacaaaatga gagaaagtaa aatggagaga 301 aggaaaaaga agatggtgac agggaattgg agaaaatcaa ggacaaggga ataacaaaag 361 gaaacacaat taaagaagag aatagatgga aagacaagga cacaaatcaa gtgcagggac 421 ataaaaaaat gttctgtgaa tgagtgtaca agatatgatg taaatcatat gcagtgtcat 481 gtttcaagaa atggaaaagg aaaaaaaaca gtgagacaaa caggcacatt ggcagacaga 541 caagtggcag atgggcatgc aatttcagca ttttttgaat tttttacatt tgtaatttaa 601 taataataat ttaatcattt aagacaattg ttaaaactct tcctccactc agcatccatg 661 tgagacactt cttgggagtc ggattgggtg ctgcatcata tgataacggt cggacaagtg 721 tgaaaaacag tacattcttt cattggatta tagtcatttg actgctgaag tccagccatc 781 tcatcactgg aagagccagg tcagtgcagc tagagatgaa tgaataattg tatctcttag 841 aaaacgagtt aataatgatg tttcctgttt tattttccat ttgcttcctg tgttttttat 901 tgatgtcaga tgccctcatg ggctccaccc ttctttacat gccaggtgtt attccccttt 961 atttttttgt gtgctgtctg ggactgcaag aagccattcc ttaggctaat gtttgccaca 1021 cagaaacaac ttgtctgatt gttgaattta tgaatgatac aagttctcca attatttttg 1081 tgaaattttt atttttatac atttctctgt gtggtactct gggcattgtt ctcttcactc 1141 ctatacagta gaaacaccac ccaatcaaat gccatcatta taggttttgt ttgacgctga 1201 acacaacgca gaacataagt tctgaagata tttttgaaaa ccaaagatgt ttgtggctgt 1261 gttcctttct cacagcggag tttgatcagt gttcttggta gaacagtggg gaagtgtaca 1321 aacactttaa ctgctgtgat tattcatgga tctcccacag attccacata ttactgaaca 1381 ttaacactca acaggacatg gctaccagtt tagagaaaat attccctgtg gaacacaaac 1441 gctgagtgaa cttctctcac agttaacctc caatcgagtc gacaagaaac ctgaagaata 1501 tctgagcttg aatttgtctg tgattggttc agatcaacac tggtcaactg atgtctgctc 1561 tcttgaagca attttcattg ttttaaatgc aaatctaaaa actaaccctg aagatgggtg 1621 ggaaatacag caagcaatac cctaacccag attgatgatt atatcagaaa tgaactagga 1681 cccagggtcc aggttcagga ctacaaacat catgtgggtt agggtcagag gttcgaaaaa 1741 gacaatggat tgttagccat ttagacacat caactccaca tctgtgtgta cgagtgtgtg 1801 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgag agagagagag agcgagaggg 1861 agtgagagag agagcgagta tgtgtgtgtc ttcatattta ctacatatta agtactccca 1921 aaggattgtt ggagggttag gacacggttg aggttggaat tgaattcagg tcagggttag 1981 tgtcagcgtc agtgtcaggg tcaggatcag tgtttgagtt atgtttaagc tgctggttag 2041 aacagaaagg aataagggta aggagctgag taatgtatta tatgtcctca aaaagacaga 2101 aatgtgaaag tgtgtgtgtg tgtgtgtgtg cgtgtgtgtg tttttgtgtg tgtgcacgtg 2161 ccaacacctt tgtcttgcct tggttacagt tagtgacatg gagtgtgtgg ttacgtgctt 2221 aagggctggt cttgagtagt tcttgtgctt cttctgtctc aggcatcatc aacctgaaag 2281 ttttcattgt tttctgagat tttgttgttg tttacagctt tatagtagca gtgtttcatt 2341 cccacaatgc attgttttgc agctcaacta tgaatcaatt attagtcttt taaattatat 2401 tatgtactcg tgttgtactt aaaagttaac tgcaatgttt caatgtttgg ttcaactctc 2461 attttattta ataaataatg cagaaagtta ctgataacta atgtcattat caactattga 2521 tcattcctgg atcctgtgag cacagtttga acacatgcaa gtgtgcgtca tcttgcagag 2581 gaactttatg aagcagtggg aagtaaagag aacgggtcat tagactgaag cagcaaacat 2641 catcatcatc atcatcatca tcatcatcat ctctttcttt tttcccctct ctaacagact 2701 cactaacatt cacatgcaca cacagcatga cgtcagcgga atgggggcta tatatgagat 2761 gagctcagat ggaaaagagc cacaagatct gcagagccag tgacagaaac acttagttgg 2821 gtaccgctag cggcgcgccg agctcggatc ctgagaactt cagggtgagt ctatgggacc 2881 cttgatgttt tctttcccct tcttttctat ggttaaattc atgtcatagg aaggggagaa 2941 gaacagggta cacatattga ccaaatcagg gtaattttgc atttgtaatt ttaaaaaatg 3001 ctttcttctt ttaatatact tttttgttta tcttatttct aatactttcc ctaatctctt 3061 tctttcaggg caataatgat acaatgtatc atgcctcttt gcaccattct aaagaataac 3121 agtgataatt tctgggttaa ggcaatagca atatttctgc atataaatat ttctgcatat 3181 aaattgtaac tgatgtaaga ggtttcatat tgctaatagc agctacaatc cagctaccat 3241 tctgctttta ttttatggtt gggataaggc tggattattc tgagtccaag ctaggccctt 3301 ttgctaatca tgttcatacc tcttatcttc ctcccacagc tcctgggcaa cgtgctggtc 3361 tgtgtgctgg cccatcactt tggcaaagca cgtgaattcg aagatctggc cgcctgggcc 3421 ggtgaccgta ccgggccccc cctcgagctc aagcttcgaa ttctgcagtc gacggtaccg 3481 cgggcccggg atccaccggt cgccaccatg gcctcctccg agaacgtcat caccgagttc 3541 atgcgcttca aggtgcgcat ggagggcacc gtgaacggcc acgagttcga gatcgagggc 3601 gagggcgagg gccgccccta cgagggccac aacaccgtga agctgaaggt gaccaagggc 3661 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tccagtacgg ctccaaggtg 3721 tacgtgaagc accccgccga catccccgac tacaagaagc tgtccttccc cgagggcttc 3781 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg cgaccgtgac ccaggactcc 3841 tccctgcagg acggctgctt catctacaag gtgaagttca tcggcgtgaa cttcccctcc 3901 gacggccccg tgatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac 3961 ccccgcgacg gcgtgctgaa gggcgagacc cacaaggccc tgaagctgaa ggacggcggc 4021 cactacctgg tggagttcaa gtccatctac atggccaaga agcccgtgca gctgcccggc 4081 tactactacg tggacgccaa gctggacatc acctcccaca acgaggacta caccatcgtg 4141 gagcagtacg agcgcaccga gggccgccac cacctgttcc tgtagcggcc gccaccgcgg 4201 tggagctgtt taaaccgtac gggaaacggc cctattctat agtgtcacct aaatgctaga 4261 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc 4321 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag 4381 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag 4441 gacagcaagg gggaggattg ggaagacaat agcgcggccg ctctagagca tggctacgta 4501 gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc 4561 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc 4621 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gccagctggc gtaatagcga 4681 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaattc 4741 cagacgattg agcgtcaaaa tgtaggtatt tccatgagcg tttttcctgt tgcaatggct 4801 ggcggtaata ttgttctgga tattaccagc aaggccgata gtttgagttc ttctactcag 4861 gcaagtgatg ttattactaa tcaaagaagt attgcgacaa cggttaattt gcgtgatgga 4921 cagactcttt tactcggtgg cctcactgat tataaaaaca cttctcagga ttctggcgta 4981 ccgttcctgt ctaaaatccc tttaatcggc ctcctgttta gctcccgctc tgattctaac 5041 gaggaaagca cgttatacgt gctcgtcaaa gcaaccatag tacgcgccct gtagcggcgc 5101 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 5161 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 5221 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 5281 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 5341 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 5401 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 5461 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 5521 attaacgttt acaatttaaa tatttgctta tacaatcttc ctgtttttgg ggcttttctg 5581 attatcaacc ggggtacata tgattgacat gctagtttta cgattaccgt tcatcgattc 5641 tcttgtttgc tccagactct caggcaatga cctgatagcc tttgtagaga cctctcaaaa 5701 atagctaccc tctccggcat gaatttatca gctagaacgg ttgaatatca tattgatggt 5761 gatttgactg tctccggcct ttctcacccg tttgaatctt tacctacaca ttactcaggc 5821 attgcattta aaatatatga gggttctaaa aatttttatc cttgcgttga aataaaggct 5881 tctcccgcaa aagtattaca gggtcataat gtttttggta caaccgattt agctttatgc 5941 tctgaggctt tattgcttaa ttttgctaat tctttgcctt gcctgtatga tttattggat 6001 gttggaattc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat 6061 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc 6121 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca 6181 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg 6241 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat 6301 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 6361 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 6421 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc 6481 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa 6541 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg 6601 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt 6661 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg 6721 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac 6781 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc 6841 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa 6901 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc 6961 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt 7021 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga 7081 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 7141 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 7201 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 7261 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 7321 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 7381 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 7441 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 7501 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 7561 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 7621 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 7681 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 7741 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 7801 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 7861 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 7921 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 7981 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 8041 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 8101 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 8161 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 8221 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg 8281 ttggccgatt cattaatg //