LOCUS       Exported                8088 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-fPV-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
            Callaway EM
  TITLE     Short Promoters in Viral Vectors Drive Selective Expression in 
            Mammalian Inhibitory Neurons, but do not Restrict Activity to 
            Specific Inhibitory Cell-Types.
  JOURNAL   Front Neural Circuits. 2009  . 3():19.
  PUBMED    19949461
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   7..136
                     /label=AAV2 ITR
     intron          2638..3112
                     /label=-beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     3194..3210
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3194..3210
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      3243..3252
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3249..3965
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3249..3965
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3294..3315)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3555..3574)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3902..3923
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4026..4044)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4027..4044)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4064..4081)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4070..4181
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4872..5385
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4959..4978)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5169..5190
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5866..5885)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5985..6007
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6045..6063)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6131..6235
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6236..7096
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6454..6473)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7267..7855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7756..7775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8009..8026
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagag gatccttcga aaagcttgta tacggccggc cacgcgtcag tttctgatac
      241 ctatttttga gctgacacga gcttgtgaaa acaactgctg gttgattgcg tgtttgcatt
      301 gtcacatctg tacagaatgt cagtggttgc acaatttctg ggggggattc tccaaactac
      361 agcgctgttt taccggactg agcaatgtca actacatatt aaaaagttaa agggcagatt
      421 ttctgtctaa cgtgcagatt atgttggatt taatcaagaa cttcatagga ctgttctctc
      481 cttgtgattc cccataattc ctacagtcta tgtgacagcg aacacatcat gctgccctct
      541 tttctcagcc ttgagcctgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
      601 gttagtgatg ggctctggag cttctctgaa cagatatttc agagcttctt acgtaacagg
      661 tcccgctgca cctcaatcct taactacaga tgtgtcccag ctgatccaaa acgggccata
      721 tgacttgatc aggtgttaac agtggtggaa aaaaccctgg gagaggacac aagcagaaaa
      781 gagcagtgca tgggtgaaag taaaaaaaac ttcatgcctg tttttagagg taataatgga
      841 gcatgtattg atttatgtgg agtgtgtagg tgtgagcatc attatttaat caaccttccc
      901 ggtgggtgct gccctgtgat tgctgatgta atgaggtacc aaaacatcaa tatctggagg
      961 tagctctgca aacatgcgat ggaagcatga aaatctgcct cggtttacag tctcgggctg
     1021 cctctcagca ctgggatgga tcacactatt tgattgaagg cacatttgcc acagctctgc
     1081 aaaggtggag caacctgcac attagctctt aagtcacaaa tcactctgca aaagtctaaa
     1141 atcaccgaga atgataaaac ttttaaaatg cagagatcct gaaaaaacac aaggcataaa
     1201 aaaagaaaga caaatatgtt gtctccatag taaccatatc tctaataaag acccgaccat
     1261 gcagatagtt ttgaccttga tatatttttt aggttgatta caacctctga aatcctaaat
     1321 gttggtgcta aaaagattta attttaacta tctgtaccag gggaaggtgg acggtttggc
     1381 ctctcattac ttaacggttt ggccgtctga caaccaaacg cccaacaact tggtggattc
     1441 cttttaaaac cgcaccatct cccacgcacg cccccacgca gcagccgtaa cattagcact
     1501 aaatatacca ctctaaccag tgcctgcagt gcagtcgtgc atgtttggca aagagtgggg
     1561 cttccttctc ccactctgct cagccaactg cctgaccagg acctacgggg tcgcgggagg
     1621 gtttgtgccg tgcagttctt gccatctcgg ttctggtcag ttttgtcaac tatattgtaa
     1681 tcaccgtgat tgacaggtga actctggttg cacagaagga ggttgaactg cacagaagga
     1741 gaacttcagc tcagactgct cacgctggca tgcctcgatc taaaataaac tctctccaac
     1801 ttttctttcc ttgacattgt aggttaaatc agaagtctga gctctgctct gaactttcca
     1861 actctttctt tctgtcatta ttgagaagcg tctctataaa accaattaga cttaaacata
     1921 catacataca tacatacata catacataca tacatacata catacataca tacatacata
     1981 catacataca tacatatggc atgtgatcaa acatatgtga tatctttgtc tgagatgcct
     2041 gagcctagga acaggttaga gggccagaac atcgtgacat acctgaactg tctgtggtaa
     2101 cttaatcaaa gataactgag ctgcaatcac tgggcagttt cagcatcttc tcacagccca
     2161 gagcacttca gcaaacactt gttaaagaag aaattagact ctccaaagac ttctgtgtca
     2221 taatcatgaa gacaagagat attttggctt cgatttgtac atcattacct gtactttgaa
     2281 gggataaaaa taaaacaaaa gcttcaacat ctggtcagca cacatggttc ggtctctgtg
     2341 tacagtcgca gacggctcag gcctgacccc tatcgacacc tgtctgctac tacaacagat
     2401 ctattacact cttcgcagtt aaaaatgatt tacttcattt aaaaaaagtc cgccatccaa
     2461 caaaacgtgt gttctggcat gcagatcaaa catcagatta gggatttttg tacactacct
     2521 tcctcagtta cagctgatat atatcttttc tgcttttcca ctgaccttta acctcctcct
     2581 gtcttttcag ctggcaaagc tagcggcgcg ccgagctcgg atcctgagaa cttcagggtg
     2641 agtctatggg acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat
     2701 agaaggagag aagtaacagg gtacacatat tgaccaaatc agggtaattt tgcatttgta
     2761 attttaaaaa atgctttctt cttttaatat acttttttgt ttatcttatt tctaatactt
     2821 tccctaatct ctttctttca gggcaataat gatacaatgt atcatgcctc tttgcaccat
     2881 tctaaagaat aacagtgata atttctgggt taaggcaata gcaatatttc tgcatataaa
     2941 tatttctgca tataaattgt aactgatgta agaggtttca tattgctaat agcagctaca
     3001 atccagctac cattctgctt ttattttatg gttgggataa ggctggatta ttctgagtcc
     3061 aagctaggcc cttttgctaa tcatgttcat acctcttatc ttcctcccac agctcctggg
     3121 caacgtgctg gtctgtgtgc tggcccatca ctttggcaaa gcacgtgaat tcgaagatct
     3181 ggccgcctgg gcctctagaa ctagtggatc ccccgggctg caggaattcg atgctaccgg
     3241 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     3301 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     3361 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     3421 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     3481 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     3541 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     3601 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     3661 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     3721 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     3781 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     3841 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     3901 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     3961 acaagtccgg actcagatcc tactagtagg atctcgagtt taaaccgtac gggaaacggc
     4021 cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct
     4081 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     4141 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     4201 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     4261 agcgcggccg ctctagagca tggctacgta gataagtagc atggcgggtt aatcattaac
     4321 tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     4381 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
     4441 gagcgagcgc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     4501 gttgcgcagc ctgaatggcg aatggaattc cagacgattg agcgtcaaaa tgtaggtatt
     4561 tccatgagcg tttttcctgt tgcaatggct ggcggtaata ttgttctgga tattaccagc
     4621 aaggccgata gtttgagttc ttctactcag gcaagtgatg ttattactaa tcaaagaagt
     4681 attgcgacaa cggttaattt gcgtgatgga cagactcttt tactcggtgg cctcactgat
     4741 tataaaaaca cttctcagga ttctggcgta ccgttcctgt ctaaaatccc tttaatcggc
     4801 ctcctgttta gctcccgctc tgattctaac gaggaaagca cgttatacgt gctcgtcaaa
     4861 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4921 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4981 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5041 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5101 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5161 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5221 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5281 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttaaa tatttgctta
     5341 tacaatcttc ctgtttttgg ggcttttctg attatcaacc ggggtacata tgattgacat
     5401 gctagtttta cgattaccgt tcatcgattc tcttgtttgc tccagactct caggcaatga
     5461 cctgatagcc tttgtagaga cctctcaaaa atagctaccc tctccggcat gaatttatca
     5521 gctagaacgg ttgaatatca tattgatggt gatttgactg tctccggcct ttctcacccg
     5581 tttgaatctt tacctacaca ttactcaggc attgcattta aaatatatga gggttctaaa
     5641 aatttttatc cttgcgttga aataaaggct tctcccgcaa aagtattaca gggtcataat
     5701 gtttttggta caaccgattt agctttatgc tctgaggctt tattgcttaa ttttgctaat
     5761 tctttgcctt gcctgtatga tttattggat gttggaattc ctgatgcggt attttctcct
     5821 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     5881 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     5941 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     6001 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     6061 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     6121 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6181 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6241 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6301 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6361 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6421 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6481 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6541 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6601 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6661 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6721 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6781 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6841 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6901 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6961 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7021 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7081 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7141 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7201 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7381 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     7441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7621 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7681 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7741 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7801 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7861 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7921 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7981 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8041 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatg
//
