LOCUS Exported 8403 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAAV-fTCAP-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8403) AUTHORS Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S, Callaway EM TITLE Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types. JOURNAL Front Neural Circuits. 2009 . 3():19. PUBMED 19949461 REFERENCE 2 (bases 1 to 8403) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8403 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 7..136 /label=AAV2 ITR intron 2952..3427 /label=-beta-globin intron /note="internally truncated intron from human beta-globin" primer_bind 3509..3525 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 3509..3525 /label=pBluescriptSK /note="For pBluescript vector" regulatory 3558..3567 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3564..4280 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3564..4280 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3609..3630) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3870..3889) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4217..4238 /label=EGFP-C /note="EGFP, forward primer" promoter complement(4341..4359) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(4342..4359) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(4379..4396) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4385..4496 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5187..5700 /direction=RIGHT /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5274..5293) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5484..5505 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6181..6200) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6300..6322 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(6360..6378) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6446..6550 /gene="bla" /label=AmpR promoter CDS 6551..7411 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6769..6788) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7582..8170 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8071..8090 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8324..8341 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg 61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc 121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca 181 tgctctagag gatccttcga aaagcttgta tacggccggc cacgcgtggc cggccatgac 241 agtgaaagag aggatggaat tttgagtgat tgatcctaag acccacctgc aataataaaa 301 gacacaaggc agggggcagc agccattagg cctggagacc caagacacaa atgcgtagct 361 tttctttccc cttatgcaga tttcaaagtt ttactgtgtc aatcttttct ttattcttct 421 gtctgcagag cttgatgcta attctgcttt gtgctgaaac atgaaagggt catcaggcga 481 atgtgacaga aatattaagt gacagttctc tccggttgag tcctttccag ttttggccct 541 gttcactgtt cagctgttgt gagccttcag gggagcagtg ttcttgatat tctgtcctgt 601 gattatcagc acttgcttcc tttgatcaca tgacccagcc cagaactctg atgtcagaat 661 atttttttta tgtttcacat gattgggttt gtgtaattgc tggatttaac ttacattcag 721 catctcagat gctttgggaa tagcagatgg cgatattacg tacggtgaac ttaaaaaccg 781 ggtttgtacg tgactgacgt ttcttccagg atgtctgtgt gtgtttaatc taacagtgaa 841 ctgaaatcaa gggtaatttc tcaaactcac atggtcgatt gtgtgtgcat gtgtgtgtgt 901 tgtggtgtcc agttcaggat gaaataaagt gatgtgaagc atgttacggg cagcgtgtca 961 ttagaaagtc aagctgcttc tttctctttt caagcgctca cgtatcctgt tggctgtcaa 1021 ccatgggctg aattactgtc tgatctgcag tcatgccaga aaatcccggc ctctctggtg 1081 tctgactgtg ccgctgggac cagtcaggga ggaagatgaa tggtgatgat agtgttttgg 1141 tgctcagctg ctatttcttg ttgttattgt tatataagag agacattgga gggggcgagg 1201 gtggcacatg tttcaacacg gcaccagagt tttagcacac acacacacac acacacacac 1261 acacacattg ctggtaaccc ttgagagtgc agtgctgaag tagatgtgca ggaagttaac 1321 tttctcgtca agcacgcaac gtcacgctga ccagccgacc ttctcgttgg tgactcaagt 1381 cgttcacttt catgatattt ggctctattt gccaccttcc gacaggcccg ggcttcatcc 1441 agcgatcgcc cttctccctc tctccctccc tctcccagtc tctctgctgg cgacagctca 1501 tttaagtgtc acttcccagc agctgtggca gccttgttat gcaacgccat gttcttccgc 1561 aatgtcggcc tgttacaggt ggagggacgg tggggggatg acgtgactta cctcatcggt 1621 gttcctccaa atggactctt tacaccctcg tctagcacga gtcttaagaa cgacatgacg 1681 aagttaaaac aataaatcat ccacccccgt tagtcactgc tacgtcaggt tcgttctgga 1741 gtgacatcat ctcaaaccaa cttactgggt ttcagaaatg gcattttatg gttgtcatgg 1801 tgtattgaat gaccccatga gtgtattctg gctctgggtt ctttcaatga caccaagcag 1861 tggtatgcaa ccctactgag aagaaattta aatacagtta aaagccattg gaaaataaca 1921 tgtaactgat gtattagaag tagatgttat tggtcagtgg agaagagtgg cggttattta 1981 agagctctag ttgtacggaa ttgctttgat cattttttaa gagaccgggc ttttgccaca 2041 tgtgtgaaat cagaaattta atgtcgaatg tggatcaatg cgccaccgag tggcaccggt 2101 ttcgcatctt tacattctgt aacctgagtc aagtagacat ggaatgaccc gctgtcaccg 2161 atgtactgaa caacggtgct gtcatggcat atttgactct cagcagttga atggttcccc 2221 gctgttttat ttctttagaa tttcagaggt tttgtcacat ttatcttaac ctcctctgtg 2281 tatttgagac cactgcgtgg ttatcttccc acattagcat gttgtcctgc tcccccatgt 2341 acattattgc ctaaacttta ttaagactct ctctaatcaa ctaaacctgt tgtttgtgtc 2401 ggtctgcatg tttatctaat ctaagtctta tttaagtcca catgtttcca ccgcgcccga 2461 gtgatcctct ctgacgtgtc aagtgtacag ctgagtgagg gacgaggtga ctggccacat 2521 aattccatag aatttgagca tcgggggatt ttcaagccta ttacccttaa cccctcgact 2581 gagtggcttc cagttctcaa caggaatgag acacagagca ctcggtgtcg gatgtctctg 2641 cacagtgatt gtcgagtgac aacatgactc agaaatagaa acagaagatc tataaaagcg 2701 gtgggtggat cagggctcgg acacagacgg ttcagaggag tgaatccggg agccaccccg 2761 agcatcacag ttgcctcatc acaggcatca cttgaccccc cgagacgcat ctttctgaga 2821 cgaatttccc tgcagcagac ataatgccag tctgttcaat cctggagaag tgtaatgatg 2881 aggtgatggg cgctgagctg acctgcagtt tggctagcgg cgcgccgagc tcggatcctg 2941 agaacttcag ggtgagtcta tgggaccctt gatgttttct ttccccttct tttctatggt 3001 taagttcatg tcataggaag gggagaagta acagggtaca catattgacc aaatcagggt 3061 aattttgcat ttgtaatttt aaaaaatgct ttcttctttt aatatacttt tttgtttatc 3121 ttatttctaa tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat 3181 gcctctttgc accattctaa agaataacag tgataatttc tgggttaagg caatagcaat 3241 atttctgcat ataaatattt ctgcatataa attgtaactg atgtaagagg tttcatattg 3301 ctaatagcag ctacaatcca gctaccattc tgcttttatt ttatggttgg gataaggctg 3361 gattattctg agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcttcct 3421 cccacagctc ctgggcaacg tgctggtctg tgtgctggcc catcactttg gcaaagcacg 3481 tgaattcgaa gatctggccg cctgggcctc tagaactagt ggatcccccg ggctgcagga 3541 attcgatgct accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg 3601 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 3661 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 3721 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 3781 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 3841 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 3901 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 3961 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 4021 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 4081 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 4141 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca 4201 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 4261 gcatggacga gctgtacaag tccggactca gatcctacta gtaggatctc gagtttaaac 4321 cgtacgggaa acggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc 4381 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg 4441 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat 4501 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag 4561 gattgggaag acaatagcgc ggccgctcta gagcatggct acgtagataa gtagcatggc 4621 gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc ctctctgcgc 4681 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg 4741 gcggcctcag tgagcgagcg agcgcgccag ctggcgtaat agcgaagagg cccgcaccga 4801 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aattccagac gattgagcgt 4861 caaaatgtag gtatttccat gagcgttttt cctgttgcaa tggctggcgg taatattgtt 4921 ctggatatta ccagcaaggc cgatagtttg agttcttcta ctcaggcaag tgatgttatt 4981 actaatcaaa gaagtattgc gacaacggtt aatttgcgtg atggacagac tcttttactc 5041 ggtggcctca ctgattataa aaacacttct caggattctg gcgtaccgtt cctgtctaaa 5101 atccctttaa tcggcctcct gtttagctcc cgctctgatt ctaacgagga aagcacgtta 5161 tacgtgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg 5221 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 5281 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 5341 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 5401 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 5461 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 5521 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 5581 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat 5641 ttaaatattt gcttatacaa tcttcctgtt tttggggctt ttctgattat caaccggggt 5701 acatatgatt gacatgctag ttttacgatt accgttcatc gattctcttg tttgctccag 5761 actctcaggc aatgacctga tagcctttgt agagacctct caaaaatagc taccctctcc 5821 ggcatgaatt tatcagctag aacggttgaa tatcatattg atggtgattt gactgtctcc 5881 ggcctttctc acccgtttga atctttacct acacattact caggcattgc atttaaaata 5941 tatgagggtt ctaaaaattt ttatccttgc gttgaaataa aggcttctcc cgcaaaagta 6001 ttacagggtc ataatgtttt tggtacaacc gatttagctt tatgctctga ggctttattg 6061 cttaattttg ctaattcttt gccttgcctg tatgatttat tggatgttgg aattcctgat 6121 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag 6181 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga 6241 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc 6301 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg 6361 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc 6421 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 6481 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 6541 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 6601 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 6661 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 6721 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 6781 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 6841 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 6901 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 6961 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 7021 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 7081 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 7141 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 7201 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 7261 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 7321 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 7381 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 7441 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 7501 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 7561 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 7621 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 7681 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 7741 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 7801 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 7861 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 7921 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 7981 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 8041 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 8101 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 8161 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 8221 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 8281 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 8341 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 8401 atg //