LOCUS Exported 8291 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAAV-fCAMK-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8291) AUTHORS Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S, Callaway EM TITLE Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types. JOURNAL Front Neural Circuits. 2009 . 3():19. PUBMED 19949461 REFERENCE 2 (bases 1 to 8291) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8291 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 7..136 /label=AAV2 ITR intron 2840..3315 /label=-beta-globin intron /note="internally truncated intron from human beta-globin" primer_bind 3397..3413 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 3397..3413 /label=pBluescriptSK /note="For pBluescript vector" regulatory 3446..3455 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3452..4168 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3452..4168 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3497..3518) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3758..3777) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4105..4126 /label=EGFP-C /note="EGFP, forward primer" promoter complement(4229..4247) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(4230..4247) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(4267..4284) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4273..4384 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5075..5588 /direction=RIGHT /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5162..5181) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5372..5393 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6069..6088) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6188..6210 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(6248..6266) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6334..6438 /gene="bla" /label=AmpR promoter CDS 6439..7299 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6657..6676) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7470..8058 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7959..7978 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8212..8229 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg 61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc 121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca 181 tgctctagag gatccttcga aaagcttgta tacggccggc cacgcgtagt ctggagacca 241 agttcaggac tccggagacc cagtccaggc ctctggggtt ccagtccagg acaataaaga 301 agcagttcag gattctaaag aaccaatcca ggatgcaggc tctcagccat gatgtaattt 361 tgcaggaaat agattttctg ctctcttctc atcctacatg aactctgcaa aatacagaat 421 tggtttctta tcatcttcac tgtgaccagg gagtggaatc attgaggcat gaacacaggt 481 cttaattctg aatgtgtttg tgtcacagac tattagcttt actcagctca tactttcaca 541 gagattaatc atgaattatg aatcatgaaa tgatcttgat gaataaatct tcacactgga 601 acatcttcat ttccgtgctt ctgttgtggg tgaaagatca ctgctccctt ctggctggat 661 aaaaaaaaaa gacatcaaga aatatgaaga tatttaaatt ataaatggtg gtagtgacga 721 ctggtggtgc agttcaaatc accaggtaat caatcgccga tttgaaatat gctgtacccg 781 cccacagccg ccaggggacg ctagtcaaca acaggacatt taaatgtcag tgaaggaatt 841 tatcctgtaa aatcttgcga tgtgccaata ttaatgccaa aaaaaactga tataacactg 901 gctggagaat gaataatcat cagatgagtt tacataagcg ataatggtgt ttggtctgtg 961 tttgaaattg ctggttttgt tattctctgc aatattgctg aattgtgaac tgaaaggttt 1021 caaccagaca cataagctga atcgaatcga tgagttttaa tgaggaaacg attgcttgtg 1081 tcaaaagagg catctaatgg taaaattgtt aagggcagct ttaagcaatt gatggaaaca 1141 gctggtgctg agctcacgag gtcacatttg catctctatc taagtgatat tgcactaatg 1201 gaagaatcca tttaaaagtt gggatcccct ccatttaata gaccacctgc gtctgacctt 1261 ctgtcaccgt gtcatctgct acagtgggaa acgatcataa agcagcatgt gacatatgtt 1321 tcctcattag tccagtgctc ccagtgcccc cagtgctgcc ttcagtgagc cgtctgttta 1381 attccacttc tgtcaaacaa acatggagtt tgaactctta acgatggtgt tttgtttgag 1441 tccagcagtc atgcttcaag caaccgtgag caggtctttc taatatgaat tctccttacc 1501 agccaattcc tgctcctcct cctcttcctc ctcctcttct gctgggacag cgaggaggct 1561 ccaaagaggg cagccatgca gaactgatga gtgacagaaa gagaggagca gaattgagag 1621 aggctgctcc cggcagacac aaacaaaccc caaaatagac taaagaacaa gaaggctcag 1681 tccctctgac ctcaggttga aaagacacta cagacagctg caatatcacc ctcgctccgc 1741 tcaccggttc agttacacac acccgattta acagtttgtt ctttttcttc acatgcagca 1801 aaccaaagtc cgctcagatg gtgaatgggt tgtaaatttg ctattgtaac cacgaacaac 1861 agagcaggtg gggtaaatct agcaccagtt gtcaggcctt ctctgtgagt ctgttcattt 1921 cgtctgtcct aaaattcctt atgctggttt tctttattcc ttcaccattg gtgggagcgt 1981 cccagttccc cccccggttc cattactgca cgtagtatcg gaccattcag tgtccttgga 2041 tacgctggac gcagactcac acatgaaatt tcaggtaaac gccgcctgtt gtgtgttgtt 2101 cgcctcccac aggtcacctc ctctctggaa aatcttactg cgagactttg tagtactggt 2161 gagtagcaag agcagcttta tcttgtagta gcactgaagt tagcacaagt ataagcagtc 2221 agatcggaag taattttgaa tgtgatggga gctgaggagt ggcgttccgg acgtgttctc 2281 tatggttttt ctatgtacaa taatatttat agtactgcag ttttatttcc cgaataatct 2341 gatgctgttt gcagttactt tccagcacac acagagggga tgcagttacc atggtgaccc 2401 tgtgcatgtg agtgtacatg catgcgtgtg tgtgtctgtc ccggtctgtg attggctgat 2461 gggccagggt gggtgggcag gtggagcgtc tgaaagcatc tttctcccac agacccggac 2521 agagagacag agagacacag atctcctaac agctctctcc ttcttccctt catcttcctc 2581 cttttcctcc tcttcctcat cctcctcctc ctcttcctct cctcgggact gaaactatct 2641 ctccatcact cttctactct ctctattttc tctcctccat ccttctggca gacagaacgg 2701 cagctctaac tcttggcctc cttcgtctcc ggcatcatca tcttcagtgg aagacggaca 2761 gggaccccct tgaccctcga cctacagggt caccagggat gctagcggcg cgccgagctc 2821 ggatcctgag aacttcaggg tgagtctatg ggacccttga tgttttcttt ccccttcttt 2881 tctatggtta agttcatgtc ataggaaggg gagaagtaac agggtacaca tattgaccaa 2941 atcagggtaa ttttgcattt gtaattttaa aaaatgcttt cttcttttaa tatacttttt 3001 tgtttatctt atttctaata ctttccctaa tctctttctt tcagggcaat aatgatacaa 3061 tgtatcatgc ctctttgcac cattctaaag aataacagtg ataatttctg ggttaaggca 3121 atagcaatat ttctgcatat aaatatttct gcatataaat tgtaactgat gtaagaggtt 3181 tcatattgct aatagcagct acaatccagc taccattctg cttttatttt atggttggga 3241 taaggctgga ttattctgag tccaagctag gcccttttgc taatcatgtt catacctctt 3301 atcttcctcc cacagctcct gggcaacgtg ctggtctgtg tgctggccca tcactttggc 3361 aaagcacgtg aattcgaaga tctggccgcc tgggcctcta gaactagtgg atcccccggg 3421 ctgcaggaat tcgatgctac cggtcgccac catggtgagc aagggcgagg agctgttcac 3481 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 3541 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 3601 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca 3661 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 3721 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 3781 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 3841 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 3901 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca 3961 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 4021 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 4081 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 4141 cactctcggc atggacgagc tgtacaagtc cggactcaga tcctactagt aggatctcga 4201 gtttaaaccg tacgggaaac ggccctattc tatagtgtca cctaaatgct agagctcgct 4261 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 4321 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 4381 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 4441 agggggagga ttgggaagac aatagcgcgg ccgctctaga gcatggctac gtagataagt 4501 agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct 4561 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct 4621 ttgcccgggc ggcctcagtg agcgagcgag cgcgccagct ggcgtaatag cgaagaggcc 4681 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa ttccagacga 4741 ttgagcgtca aaatgtaggt atttccatga gcgtttttcc tgttgcaatg gctggcggta 4801 atattgttct ggatattacc agcaaggccg atagtttgag ttcttctact caggcaagtg 4861 atgttattac taatcaaaga agtattgcga caacggttaa tttgcgtgat ggacagactc 4921 ttttactcgg tggcctcact gattataaaa acacttctca ggattctggc gtaccgttcc 4981 tgtctaaaat ccctttaatc ggcctcctgt ttagctcccg ctctgattct aacgaggaaa 5041 gcacgttata cgtgctcgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc 5101 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 5161 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 5221 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 5281 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 5341 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 5401 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 5461 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 5521 tttacaattt aaatatttgc ttatacaatc ttcctgtttt tggggctttt ctgattatca 5581 accggggtac atatgattga catgctagtt ttacgattac cgttcatcga ttctcttgtt 5641 tgctccagac tctcaggcaa tgacctgata gcctttgtag agacctctca aaaatagcta 5701 ccctctccgg catgaattta tcagctagaa cggttgaata tcatattgat ggtgatttga 5761 ctgtctccgg cctttctcac ccgtttgaat ctttacctac acattactca ggcattgcat 5821 ttaaaatata tgagggttct aaaaattttt atccttgcgt tgaaataaag gcttctcccg 5881 caaaagtatt acagggtcat aatgtttttg gtacaaccga tttagcttta tgctctgagg 5941 ctttattgct taattttgct aattctttgc cttgcctgta tgatttattg gatgttggaa 6001 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc 6061 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca 6121 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg 6181 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga 6241 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct 6301 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 6361 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 6421 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 6481 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 6541 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 6601 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 6661 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 6721 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 6781 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 6841 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 6901 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 6961 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 7021 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 7081 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 7141 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 7201 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 7261 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 7321 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 7381 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 7441 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 7501 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 7561 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 7621 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 7681 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 7741 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 7801 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 7861 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 7921 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 7981 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 8041 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 8101 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 8161 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 8221 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 8281 attcattaat g //