LOCUS Exported 8128 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAAV-hADM-RFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8128) AUTHORS Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S, Callaway EM TITLE Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types. JOURNAL Front Neural Circuits. 2009 . 3():19. PUBMED 19949461 REFERENCE 2 (bases 1 to 8128) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8128 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 7..136 /label=AAV2 ITR intron 2802..3277 /label=-beta-globin intron /note="internally truncated intron from human beta-globin" regulatory 3353..3362 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3359..4036 /codon_start=1 /product="rapidly maturing tetrameric variant of DsRed fluorescent protein (Bevis and Glick, 2002)" /label=DsRed-Express /note="mammalian codon-optimized" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH LFL" primer_bind complement(3489..3507) /label=mCherry-R /note="mCherry, reverse primer" primer_bind complement(3539..3559) /label=DsRed1-N /note="DsRed1, reverse primer" primer_bind 3757..3776 /label=mCherry-F /note="mCherry, forward primer" primer_bind 3951..3974 /label=DsRed1-C /note="DsRed1, forward primer" promoter complement(4066..4084) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(4067..4084) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(4104..4121) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4110..4221 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4912..5425 /direction=RIGHT /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4999..5018) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5209..5230 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5906..5925) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6025..6047 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(6085..6103) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6171..6275 /gene="bla" /label=AmpR promoter CDS 6276..7136 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6494..6513) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7307..7895 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7796..7815 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8049..8066 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg 61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc 121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca 181 tgctctagag gatccttcga aaagcttgta tacagagtga aaaggcagct aagccagcag 241 tgcgcggccc agacagacca ctctgggcaa ggtccgcgcg acccactgcc cagccgcctt 301 tgcaccggag ctcagagcca tatggcagag taggaaggaa gcttaaaggt ctgctgggga 361 ccggctctaa gatggggact cgagagatgg gacatgactc gcccaccttc acacagcgaa 421 ttgccgggag gtgaggacta gaagcctgtt tggctagtgg tctcgcacct ttggcctctc 481 cgtcgagtcg ctgggcttca ggacattcga ttagaggagt ggttagtagg accccagaaa 541 cccacaagca cgaagcagga agccccgtgg cttagccttt ccttcccagt ttggccccca 601 ggagagaagt aagaaagaga agaagctgtg atgaaagagc acaaacgggt gacaaacgtg 661 tctagcgtga ttcatcatga acaggcacaa attctttggg cgggggctag gactctcctt 721 tgccccttga gaagttggtg accccagcct agaggaatcc acgccgcgcc ccagtcggcc 781 gtgctagcgt gtcgggccga gtagggactc tgctgcttcc tacctgcagg gtgacttccc 841 ctctctgcag atacctcccc tctctgagct tgaattttct acctgctgaa atgggaaaag 901 gaatgttacc ttccttgcct gactcaaggg tggctgtgaa gctcaaagta gactgggatg 961 tggccgtgct tggtaaactg taaaatgatt agcatacgtg aagcgttagt gtgctccctg 1021 gcagtcagtc ctcacgttta cgatggatta atgaaggcag ccaggcacaa tctcaggtta 1081 tgaccttata aggcataata ggattacaag gaggcaaaat gaaacagtat atttcctcct 1141 aattttggtt attcctatgt ttccaaatag aaatgaaaat tctgaaattt cagataattc 1201 ccccctccaa atcccaattc tcaatttagc ttttctgagc cttgtcccta ccgtgaaatc 1261 ttactagctc accccgaaac gccggggtct tggtcctctg ggggactagt gagtcctgct 1321 cctctcggct ttgctccgtc cctgccccgg cccctccgcg ggagtctggg gtcgcggccc 1381 ggtcaggcgc caagccggca ggggggcgcg gtccacttga ggccacagct ccccaggtcc 1441 agggctcggg ccatccgcgc tgtcccttcc gcgggctctt gctgttcttc gccaggaggc 1501 tttgcactcc gggccgcatg ggtcctggat aaggacctca agaggttgta gtggcgcccc 1561 ttcgctccgg cgtactgtct gaaccctgtg cccaaagagg gctgtggcag ttcctgcacc 1621 aaccgcctgg agcccatacc taagccctct gggcacgagg gacctcctct cccccgctcc 1681 cccctccccc catcccaact ccagccccaa aggaagcaat gcgcgcgtcc gagagcagga 1741 gcgcgcgtgg ctgaggaaag aaagggaagg caaccgggca gcccaggccc cgccccgccg 1801 ctcccccacc cgtgcgctta taaagcacag gaaccagagc tggccactca gtggtttctt 1861 ggtgacactg gatagaacag ctcaagcctt gccacttcgg gcttctcact gcagctgggc 1921 ttggacttcg gagttttgcc attgccagtg ggacgtctga gactttctcc ttcaagtact 1981 tggcagatca ctctcttagc aggtaggtgc cgcagaccct gcgggttaag aggtggggtg 2041 gggggcagtg cttgccaagg ccctaacctg ggagcgctgg gtgaggggaa caacccactt 2101 tggagggttc tctgagagat agatacaccc catatcctgg gcccagctcg tgcacacagc 2161 tggaggtcca gagacccagt cccctctgct ccgtcagcca agttccaaga agttgagcag 2221 agaccctctg ggagcctggc ggggtgcagc ggcctcccct gcggggcctg tcacccggcc 2281 ggcgcgtgca aacgcctctg gcgcctctct gcgcggaggg agataagcgt ctgagccagg 2341 gaaagcgcgg gctaaacccg cctcgccggg gcccctgccc gccctccgtg ccccgcccgg 2401 gcggtgcagc tggcccgggt gctcacgctc gactctcttt cttcttttcc agggtctgcg 2461 cttcgcagcc gggatgaagc tggtttccgt cgccctgatg tacctgggtt cgctcgcctt 2521 cctaggcgct gacaccgctc ggttggatgt cgcgtcggag tttcgaaaga agtgagtccg 2581 ggcagcgcct tcccccttgc tggtacctgg caggcaaggg gaactgaccg ttggtcccga 2641 aggtctagaa gtgaatggga gcagggacag gcctgggcgt cacctgaacg cacgcgaatc 2701 gggtctgctt gtgttttcca ggtggaataa gtgggctctg agtcgtggga agagggaact 2761 gcggatgtgg cgcgccgagc tcggatcctg agaacttcag ggtgagtcta tgggaccctt 2821 gatgttttct ttccccttct tttctatggt taagttcatg tcataggaag gggagaagta 2881 acagggtaca catattgacc aaatcagggt aattttgcat ttgtaatttt aaaaaatgct 2941 ttcttctttt aatatacttt tttgtttatc ttatttctaa tactttccct aatctctttc 3001 tttcagggca ataatgatac aatgtatcat gcctctttgc accattctaa agaataacag 3061 tgataatttc tgggttaagg caatagcaat atttctgcat ataaatattt ctgcatataa 3121 attgtaactg atgtaagagg tttcatattg ctaatagcag ctacaatcca gctaccattc 3181 tgcttttatt ttatggttgg gataaggctg gattattctg agtccaagct aggccctttt 3241 gctaatcatg ttcatacctc ttatcttcct cccacagctc ctgggcaacg tgctggtctg 3301 tgtgctggcc catcactttg gcaaagcacg tgggccgcct cggccaccgg tcgccaccat 3361 ggcctcctcc gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc 3421 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 3481 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct 3541 gtccccccag ttccagtacg gctccaaggt gtacgtgaag caccccgccg acatccccga 3601 ctacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 3661 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggctcct tcatctacaa 3721 ggtgaagttc atcggcgtga acttcccctc cgacggcccc gtaatgcaga agaagactat 3781 gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat 3841 ccacaaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca agtccatcta 3901 catggccaag aagcccgtgc agctgcccgg ctactactac gtggactcca agctggacat 3961 cacctcccac aacgaggact acaccatcgt ggagcagtac gagcgcgccg agggccgcca 4021 ccacctgttc ctgtagagtt taaaccgtac gggaaacggc cctattctat agtgtcacct 4081 aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 4141 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 4201 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 4261 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcgcggccg ctctagagca 4321 tggctacgta gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat 4381 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt 4441 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gccagctggc 4501 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg 4561 aatggaattc cagacgattg agcgtcaaaa tgtaggtatt tccatgagcg tttttcctgt 4621 tgcaatggct ggcggtaata ttgttctgga tattaccagc aaggccgata gtttgagttc 4681 ttctactcag gcaagtgatg ttattactaa tcaaagaagt attgcgacaa cggttaattt 4741 gcgtgatgga cagactcttt tactcggtgg cctcactgat tataaaaaca cttctcagga 4801 ttctggcgta ccgttcctgt ctaaaatccc tttaatcggc ctcctgttta gctcccgctc 4861 tgattctaac gaggaaagca cgttatacgt gctcgtcaaa gcaaccatag tacgcgccct 4921 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 4981 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 5041 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 5101 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 5161 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 5221 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 5281 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 5341 ttaacaaaat attaacgttt acaatttaaa tatttgctta tacaatcttc ctgtttttgg 5401 ggcttttctg attatcaacc ggggtacata tgattgacat gctagtttta cgattaccgt 5461 tcatcgattc tcttgtttgc tccagactct caggcaatga cctgatagcc tttgtagaga 5521 cctctcaaaa atagctaccc tctccggcat gaatttatca gctagaacgg ttgaatatca 5581 tattgatggt gatttgactg tctccggcct ttctcacccg tttgaatctt tacctacaca 5641 ttactcaggc attgcattta aaatatatga gggttctaaa aatttttatc cttgcgttga 5701 aataaaggct tctcccgcaa aagtattaca gggtcataat gtttttggta caaccgattt 5761 agctttatgc tctgaggctt tattgcttaa ttttgctaat tctttgcctt gcctgtatga 5821 tttattggat gttggaattc ctgatgcggt attttctcct tacgcatctg tgcggtattt 5881 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc 5941 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 6001 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 6061 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 6121 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 6181 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 6241 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 6301 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 6361 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 6421 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 6481 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 6541 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 6601 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 6661 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 6721 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 6781 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 6841 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 6901 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 6961 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 7021 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 7081 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 7141 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 7201 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 7261 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 7321 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 7381 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 7441 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 7501 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 7561 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 7621 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 7681 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 7741 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 7801 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 7861 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 7921 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 7981 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 8041 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 8101 tccccgcgcg ttggccgatt cattaatg //