LOCUS       Exported                8411 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hST3GAL6-RFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
            Callaway EM
  TITLE     Short Promoters in Viral Vectors Drive Selective Expression in 
            Mammalian Inhibitory Neurons, but do not Restrict Activity to 
            Specific Inhibitory Cell-Types.
  JOURNAL   Front Neural Circuits. 2009  . 3():19.
  PUBMED    19949461
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8411
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   7..136
                     /label=AAV2 ITR
     promoter        2988..3026
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          3085..3560
                     /label=-beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      3636..3645
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3642..4319
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(3772..3790)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(3822..3842)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     4040..4059
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4234..4257
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        complement(4349..4367)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4350..4367)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4387..4404)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4393..4504
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5195..5708
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5282..5301)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5492..5513
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6189..6208)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6308..6330
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6368..6386)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6454..6558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6559..7419
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6777..6796)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7590..8178
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8079..8098
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8332..8349
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagag gatccttcga aaagcttagc cctatatgta cactgcctga aaattataat
      241 tttaggggcc tttgaaagac cttgcatgtc ccaagagtcc tctccttctt tgtggctttt
      301 cccctcccag cttattagaa gaaatcttct accaaaaggc caaattaaat atacatatat
      361 tgggagggta ttttctccaa aacaatgacc aaaaacacta acccagagag gactaaaaac
      421 ttttaattcc atcaccctac ttaagccttt gtcagtttcc acaggaatat aaaatattta
      481 tataatagca atcatgtgcc catacaataa tatatcttga atttttaaaa attaatgtta
      541 cactgatcaa aagcccctcc catcacagag agttacatag cagtgatatc tcagaaagaa
      601 caatgatttt ggcctcaggt gctttccttt gagtcacagc tccaatgtag gcagcacagc
      661 cctagaccac ctgactctgt tccttccttt ctaccatgac tggcttgcag ggtgagtgag
      721 aggaacagat acaatgcatg gtgggtggct gactcatggt gggcaatgac tggtagttaa
      781 tatttttagc gttattttta aagtcaacat tttacttcct gaattgatgg ttttgggggc
      841 aagcactgtg ccaagccttc tttacaacag agactgtttt aatcctaatc acccttcaag
      901 actagctgta ttatctcact ttacacctga caaaaaatga agcccactgt ggtaatgcca
      961 cttgcccaaa gtcacacagc aggtcagcag aagaggtagc ttttgaacta ggttggggtc
     1021 aacagtagtc actactggcc actaccaaat atgaaataca aacgtcacct cagaacagca
     1081 gatagcctct ctccttagca cactgctttg cagattcaag aagtccagaa ggccagagtg
     1141 gatgaggtga aatacagttt tagtttttaa gatcatgaac aaggggtcag tgaagaaccc
     1201 aggagaaatt aaaagagcaa agtaaacctc aacaccaatt agttcagata gacaaattcc
     1261 ctaaaactct ttcactatcc tgatgtctca agagccacgt ttaaaattac aaaatctgtg
     1321 tgggttatgt caaaggcaat ctgtagaaag ggcaaagaat atgaattatc tgaacccatt
     1381 gagttttttg tttgtttgtt tgtttgtttg tttgttttga gacagaggct cgctgtgtcg
     1441 cccaagctgg agtgcagtgg cgccatctca gctcactggg ttcaagcgat tcttctgccc
     1501 cagcttcctg agtagctgag attacaggcg cgcaccacca tgcccggcta atttttgtat
     1561 ttttaggaga gacggggctt caccacgttg gtcaggctga tctggaactc cggacctcgt
     1621 gatccgcccg cctcggcctt cgaaagtgct gggattacag gtgtgagcca cggcgcccgg
     1681 ccattttttt tttaaagtaa ggttgggaaa acaacaaatg atacctccta gggctgttat
     1741 tacaattaag acaaatacca gagcactttg gcaggtggag gcgagcggat catttgaggt
     1801 caggagttcg aaaccagcct ggccaacata atgaaacctc atctctacta aaaatacaaa
     1861 aattagctgg gagtggtaat cccagcctgt aatcccagct actcgggagg ctgaggcagg
     1921 agagtcgctt gagcctggga ggcagaggtt gcagtgagcc aagatcatgc ccctgtaccc
     1981 cagcctgggt gacaaagcaa gattctgtct caaaaaaaaa aaaaaaaaaa aggacaaata
     2041 ccagaaagcc catcacagat gaaggacttt gttccagttc gtgacacatt gccaactttg
     2101 aattacatcc aagtccgtgg gtcttctctc tggggtcaat tgatggcacg tgattacact
     2161 gctcagggcc caaaggttgg agctatgact tgaatgtttc tagacaagga attatttgac
     2221 aaacacactt taaaaagtat tcagagccat ccaagtaaga ggacattaat aacgtagggt
     2281 tttgcctatg gagagaaaag aaaacgcaaa cagcacgttc caccttcgtt ctccctaaag
     2341 taatttatct ccttcaaaag ataaaaagta cgaacacatt tgcccttgaa gggcagagcc
     2401 ccggaattca ggagctctct gaacactcct ccccttctga tagggaccaa accttctgct
     2461 gagccttgtg tcacagtcag tccgcggcgc cacaagcctc agtgtttact tctgccgcct
     2521 gccagccggg cggggaaggg gagtacccgg ccacccctca ctttagctcc tgcagctttc
     2581 caaacaaacc agggcgggat gggagtgtct tcttggctgt ggaaatcgat cctcctacct
     2641 tgtcctgaag ccacccgccg cctttcctcg tccctccctt cgcggtgatt ttccggtccc
     2701 ggcgcccggc gagggcgcgc ggctggtgtc cgccccgccc gaaaggtagg ggttcggctg
     2761 tgctcccgcc tggtccccaa cgcctcaccc tccgggagac ccgcagcctc ccaccacttt
     2821 gcaccttcct cctcgaattt gggggcggcc accgtgcaac tggcgccagc cgcggagcag
     2881 gaggagcttc ccgcgactcc ggaggcgctc ggcgcagtcg cccgggattg gggctggcgg
     2941 gagcctgaga ctccgggcag ggctgctccc tcctctgctt taattaaggt aggcgtgtac
     3001 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgcgccg
     3061 agctcggatc ctgagaactt cagggtgagt ctatgggacc cttgatgttt tctttcccct
     3121 tcttttctat ggttaagttc atgtcatagg aaggggagaa gtaacagggt acacatattg
     3181 accaaatcag ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac
     3241 ttttttgttt atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga
     3301 tacaatgtat catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta
     3361 aggcaatagc aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag
     3421 aggtttcata ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt
     3481 tgggataagg ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac
     3541 ctcttatctt cctcccacag ctcctgggca acgtgctggt ctgtgtgctg gcccatcact
     3601 ttggcaaagc acgtgggccg cctcggccac cggtcgccac catggcctcc tccgaggacg
     3661 tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt
     3721 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     3781 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttccagt
     3841 acggctccaa ggtgtacgtg aagcaccccg ccgacatccc cgactacaag aagctgtcct
     3901 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     3961 tgacccagga ctcctccctg caggacggct ccttcatcta caaggtgaag ttcatcggcg
     4021 tgaacttccc ctccgacggc cccgtaatgc agaagaagac tatgggctgg gaggcctcca
     4081 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccacaag gccctgaagc
     4141 tgaaggacgg cggccactac ctggtggagt tcaagtccat ctacatggcc aagaagcccg
     4201 tgcagctgcc cggctactac tacgtggact ccaagctgga catcacctcc cacaacgagg
     4261 actacaccat cgtggagcag tacgagcgcg ccgagggccg ccaccacctg ttcctgtaga
     4321 gtttaaaccg tacgggaaac ggccctattc tatagtgtca cctaaatgct agagctcgct
     4381 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     4441 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     4501 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     4561 agggggagga ttgggaagac aatagcgcgg ccgctctaga gcatggctac gtagataagt
     4621 agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct
     4681 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     4741 ttgcccgggc ggcctcagtg agcgagcgag cgcgccagct ggcgtaatag cgaagaggcc
     4801 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa ttccagacga
     4861 ttgagcgtca aaatgtaggt atttccatga gcgtttttcc tgttgcaatg gctggcggta
     4921 atattgttct ggatattacc agcaaggccg atagtttgag ttcttctact caggcaagtg
     4981 atgttattac taatcaaaga agtattgcga caacggttaa tttgcgtgat ggacagactc
     5041 ttttactcgg tggcctcact gattataaaa acacttctca ggattctggc gtaccgttcc
     5101 tgtctaaaat ccctttaatc ggcctcctgt ttagctcccg ctctgattct aacgaggaaa
     5161 gcacgttata cgtgctcgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     5221 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5281 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5341 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5401 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5461 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5521 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5581 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5641 tttacaattt aaatatttgc ttatacaatc ttcctgtttt tggggctttt ctgattatca
     5701 accggggtac atatgattga catgctagtt ttacgattac cgttcatcga ttctcttgtt
     5761 tgctccagac tctcaggcaa tgacctgata gcctttgtag agacctctca aaaatagcta
     5821 ccctctccgg catgaattta tcagctagaa cggttgaata tcatattgat ggtgatttga
     5881 ctgtctccgg cctttctcac ccgtttgaat ctttacctac acattactca ggcattgcat
     5941 ttaaaatata tgagggttct aaaaattttt atccttgcgt tgaaataaag gcttctcccg
     6001 caaaagtatt acagggtcat aatgtttttg gtacaaccga tttagcttta tgctctgagg
     6061 ctttattgct taattttgct aattctttgc cttgcctgta tgatttattg gatgttggaa
     6121 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     6181 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     6241 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     6301 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     6361 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6421 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6481 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6541 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6601 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6661 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6721 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6781 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6841 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6901 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6961 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7021 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7081 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7141 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7201 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7261 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7321 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7381 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7441 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7501 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7561 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7621 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7681 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     7741 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7801 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7861 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7921 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7981 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8041 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8101 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8161 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8221 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8281 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8341 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8401 attcattaat g
//
