LOCUS Exported 8129 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAAV-hNR2F2-RFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8129) AUTHORS Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S, Callaway EM TITLE Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types. JOURNAL Front Neural Circuits. 2009 . 3():19. PUBMED 19949461 REFERENCE 2 (bases 1 to 8129) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8129 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 7..136 /label=AAV2 ITR CDS 371..382 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" intron 2803..3278 /label=-beta-globin intron /note="internally truncated intron from human beta-globin" regulatory 3354..3363 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3360..4037 /codon_start=1 /product="rapidly maturing tetrameric variant of DsRed fluorescent protein (Bevis and Glick, 2002)" /label=DsRed-Express /note="mammalian codon-optimized" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH LFL" primer_bind complement(3490..3508) /label=mCherry-R /note="mCherry, reverse primer" primer_bind complement(3540..3560) /label=DsRed1-N /note="DsRed1, reverse primer" primer_bind 3758..3777 /label=mCherry-F /note="mCherry, forward primer" primer_bind 3952..3975 /label=DsRed1-C /note="DsRed1, forward primer" promoter complement(4067..4085) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(4068..4085) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(4105..4122) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4111..4222 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4913..5426 /direction=RIGHT /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5000..5019) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5210..5231 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5907..5926) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6026..6048 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(6086..6104) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6172..6276 /gene="bla" /label=AmpR promoter CDS 6277..7137 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6495..6514) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7308..7896 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7797..7816 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8050..8067 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg 61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc 121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca 181 tgctctagag gatccttcga aattggtccg tcttgactgt ccatataaaa caatttaatt 241 agggtgcttt cttgctttgg agagcaaaga aaaaaaaaaa aaaaaccctg cccagtgctt 301 attgtattta gcaccaatac aggcagaaaa tgtagcacaa gtttttggtt gaaagagcac 361 ctctgtgccc attgaagggc gttgatccga aaagacagaa aggacaagta aaatcgattt 421 gaagcgaccc tctctgtccc tttcacacca ttcagaaact ccattccttc taagacaaag 481 accagcgcct tgggaattct cagggaaaaa gaggactcta gtcccacaag cctagggcac 541 ttttttaaaa aaaaatcctc aatatttcac ttaaaaccca cacttgatgc tgtgcccccc 601 aagtatgggt tccacagatg ccccctccaa agtgccaagc tgcaaggctg acaaaccccc 661 tttcccctgc ctgtttggcc ttgtagccag gccaggacag ccgtttaggg ttagtgcagc 721 cctcaccttt gacctcctag atccgagtgg ctgccagcac tacaactttg gccaaagtta 781 aaagaaaacc acaacaaaaa attttttctg ctcccaaacc cagggaaacc agttctggtt 841 cagccgcctg agccacctta cccaatagca acctccatcc ctaacttttt tttcttcttc 901 ttttgcaatg aactgcctga gccatctctg aaggagggaa ttgaagttgt cagggctcca 961 cggctgggtt gtgggtgggg gtgagtttct tggtctaact ggtcctccat taggaacccc 1021 cgaatgccag gttatcccac tggtgcccga gctggggaag gcagtcatct atcagaactg 1081 gcaggaaagg gagaagggag aggaatgaga tgggcaggga ggggagagaa aaggccaggc 1141 tgtgatcatc ttcacctgcc ctgagtccag ccttcctgtt caactattcc tcgaccttcg 1201 gctggggatg aagggggcgg gggcgaaagg gagggggcac ccccgagtta cacaagtggc 1261 ctccttctac taagagaatg agttcacctc ctccccagag gagcctcacc atccatccac 1321 tcagtataac tgatgagaca aaccgcaagg aaaaacttct gtagcccata tttatggtat 1381 taatgaatag ttaaaaaaga tttacacttc ttatgatcaa tattctgagt tattttgctc 1441 aaagtatata attacagtgt cttcagagtt ttcatcattg tttgaggtgg caaaaccatt 1501 tgtaattttc attagccagt aaacatgtaa ttaacattca gcactgtgtt taacttaatt 1561 cctgtttaag acaaggacaa gaagcagccg aagagcgtta gttccatttg tcaagtctga 1621 catacatctt accataacag tttcctaaaa taaaagatcc gtgtttcccc attattcttc 1681 tcttaaaaca aaacaaaaca aaaatccatt agttaagaca gtattaaact gtgagaggca 1741 gcatagttta tattcaattt tcactagtta ccgtattctt gcttccttta ttcgttttgg 1801 ggagtagaga aagggagggg gtgaatgaag cattcattat ctgaaggcat ggaatgggga 1861 acccctcccc ccaaaagaaa tagatttcct cctcaggaaa gttctagaat aaggaccgga 1921 aaggggggag gcagaaatcc acaggggaga gggggaaaaa tcacttgggg agacttgagt 1981 taaaagtaga aaatgaggat tccaaaggat tcctaggaaa aatgttgccc tcttgtctca 2041 agttctatct ttgatccagg acgagggaag cctgtctgcc agtctgcgag gctgatgctt 2101 tttccccctc ctcttccacc tctgtttttt cccaacttgc tttaggatta gcctccatct 2161 ctctcgaccc caaacagtgg aatttggctg tgccttgggt cgtcttgctt tgcaatatcg 2221 cctatagttg ttggcggttt tctgctgagg ctgagccgtt tgctccagcc tccgactaaa 2281 ctcattaagt tgggagattt ttttcaattg gacgggtgtt tttaaagtct cgtttttcca 2341 gccccaaaca aggtgtaaca acgcactctt ccttctaagg aatgagatga gagacaagga 2401 tcactccaga catctcctac ctacggtttg gggttttttt tcttaaaggc gaggcttgca 2461 ttcctcagca gctatgtaca aagctccctg aaaccttgtc tctctaaagt tagtgtgcag 2521 ggttttccaa ggctgagaga gcctaataca tggggaagca cttccttgag gtggaagatc 2581 tctcccttca cctttcctct ttttccctgc aggctagtgc ctacttttta tcagtttgca 2641 caatcgctta gataaacacc gaggaggaga ttctctttaa ttatcaaaga cacatctttt 2701 cagggggcca acaaagcatt tatttcaccc gccaaactaa aggagagtta ttccagttta 2761 ggaggaagag gcgcgccgag ctcggatcct gagaacttca gggtgagtct atgggaccct 2821 tgatgttttc tttccccttc ttttctatgg ttaagttcat gtcataggaa ggggagaagt 2881 aacagggtac acatattgac caaatcaggg taattttgca tttgtaattt taaaaaatgc 2941 tttcttcttt taatatactt ttttgtttat cttatttcta atactttccc taatctcttt 3001 ctttcagggc aataatgata caatgtatca tgcctctttg caccattcta aagaataaca 3061 gtgataattt ctgggttaag gcaatagcaa tatttctgca tataaatatt tctgcatata 3121 aattgtaact gatgtaagag gtttcatatt gctaatagca gctacaatcc agctaccatt 3181 ctgcttttat tttatggttg ggataaggct ggattattct gagtccaagc taggcccttt 3241 tgctaatcat gttcatacct cttatcttcc tcccacagct cctgggcaac gtgctggtct 3301 gtgtgctggc ccatcacttt ggcaaagcac gtgggccgcc tcggccaccg gtcgccacca 3361 tggcctcctc cgaggacgtc atcaaggagt tcatgcgctt caaggtgcgc atggagggct 3421 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca 3481 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc 3541 tgtcccccca gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg 3601 actacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg 3661 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggctcc ttcatctaca 3721 aggtgaagtt catcggcgtg aacttcccct ccgacggccc cgtaatgcag aagaagacta 3781 tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga 3841 tccacaaggc cctgaagctg aaggacggcg gccactacct ggtggagttc aagtccatct 3901 acatggccaa gaagcccgtg cagctgcccg gctactacta cgtggactcc aagctggaca 3961 tcacctccca caacgaggac tacaccatcg tggagcagta cgagcgcgcc gagggccgcc 4021 accacctgtt cctgtagagt ttaaaccgta cgggaaacgg ccctattcta tagtgtcacc 4081 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 4141 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 4201 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 4261 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcgcggcc gctctagagc 4321 atggctacgt agataagtag catggcgggt taatcattaa ctacaaggaa cccctagtga 4381 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg 4441 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgccagctgg 4501 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 4561 gaatggaatt ccagacgatt gagcgtcaaa atgtaggtat ttccatgagc gtttttcctg 4621 ttgcaatggc tggcggtaat attgttctgg atattaccag caaggccgat agtttgagtt 4681 cttctactca ggcaagtgat gttattacta atcaaagaag tattgcgaca acggttaatt 4741 tgcgtgatgg acagactctt ttactcggtg gcctcactga ttataaaaac acttctcagg 4801 attctggcgt accgttcctg tctaaaatcc ctttaatcgg cctcctgttt agctcccgct 4861 ctgattctaa cgaggaaagc acgttatacg tgctcgtcaa agcaaccata gtacgcgccc 4921 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 4981 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 5041 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 5101 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 5161 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 5221 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 5281 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 5341 tttaacaaaa tattaacgtt tacaatttaa atatttgctt atacaatctt cctgtttttg 5401 gggcttttct gattatcaac cggggtacat atgattgaca tgctagtttt acgattaccg 5461 ttcatcgatt ctcttgtttg ctccagactc tcaggcaatg acctgatagc ctttgtagag 5521 acctctcaaa aatagctacc ctctccggca tgaatttatc agctagaacg gttgaatatc 5581 atattgatgg tgatttgact gtctccggcc tttctcaccc gtttgaatct ttacctacac 5641 attactcagg cattgcattt aaaatatatg agggttctaa aaatttttat ccttgcgttg 5701 aaataaaggc ttctcccgca aaagtattac agggtcataa tgtttttggt acaaccgatt 5761 tagctttatg ctctgaggct ttattgctta attttgctaa ttctttgcct tgcctgtatg 5821 atttattgga tgttggaatt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt 5881 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag 5941 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc 6001 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca 6061 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc 6121 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 6181 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 6241 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 6301 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 6361 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 6421 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 6481 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 6541 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 6601 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 6661 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 6721 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 6781 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 6841 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 6901 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 6961 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 7021 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 7081 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 7141 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 7201 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 7261 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 7321 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 7381 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 7441 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 7501 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 7561 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 7621 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 7681 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 7741 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 7801 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 7861 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 7921 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 7981 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 8041 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 8101 ctccccgcgc gttggccgat tcattaatg //