LOCUS Exported 5698 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAAV-DR1-NRSE-RFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5698) AUTHORS Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S, Callaway EM TITLE Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types. JOURNAL Front Neural Circuits. 2009 . 3():19. PUBMED 19949461 REFERENCE 2 (bases 1 to 5698) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5698 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 7..136 /label=AAV2 ITR promoter 275..313 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" intron 372..847 /label=-beta-globin intron /note="internally truncated intron from human beta-globin" regulatory 923..932 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 929..1606 /codon_start=1 /product="rapidly maturing tetrameric variant of DsRed fluorescent protein (Bevis and Glick, 2002)" /label=DsRed-Express /note="mammalian codon-optimized" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH LFL" primer_bind complement(1059..1077) /label=mCherry-R /note="mCherry, reverse primer" primer_bind complement(1109..1129) /label=DsRed1-N /note="DsRed1, reverse primer" primer_bind 1327..1346 /label=mCherry-F /note="mCherry, forward primer" primer_bind 1521..1544 /label=DsRed1-C /note="DsRed1, forward primer" promoter complement(1636..1654) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(1637..1654) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(1674..1691) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1680..1791 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 2482..2995 /direction=RIGHT /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2569..2588) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2779..2800 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3476..3495) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3595..3617 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(3655..3673) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 3741..3845 /gene="bla" /label=AmpR promoter CDS 3846..4706 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4064..4083) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4877..5465 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5366..5385 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5619..5636 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg 61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc 121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca 181 tgctctagag gatccttcga aaagcttgta tacttcagca ccacggacag cgcccctgca 241 ggtctagggg tcaaaggtca ccggtcttaa ttaaggtagg cgtgtacggt gggaggtcta 301 tataagcaga gctggtttag tgaaccgtca gatccgctag cgcgccgagc tcggatcctg 361 agaacttcag ggtgagtcta tgggaccctt gatgttttct ttccccttct tttctatggt 421 taagttcatg tcataggaag gggagaagta acagggtaca catattgacc aaatcagggt 481 aattttgcat ttgtaatttt aaaaaatgct ttcttctttt aatatacttt tttgtttatc 541 ttatttctaa tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat 601 gcctctttgc accattctaa agaataacag tgataatttc tgggttaagg caatagcaat 661 atttctgcat ataaatattt ctgcatataa attgtaactg atgtaagagg tttcatattg 721 ctaatagcag ctacaatcca gctaccattc tgcttttatt ttatggttgg gataaggctg 781 gattattctg agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcttcct 841 cccacagctc ctgggcaacg tgctggtctg tgtgctggcc catcactttg gcaaagcacg 901 tgggccgcct cggccaccgg tcgccaccat ggcctcctcc gaggacgtca tcaaggagtt 961 catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg 1021 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg 1081 cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt 1141 gtacgtgaag caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt 1201 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc 1261 ctccctgcag gacggctcct tcatctacaa ggtgaagttc atcggcgtga acttcccctc 1321 cgacggcccc gtaatgcaga agaagactat gggctgggag gcctccaccg agcgcctgta 1381 cccccgcgac ggcgtgctga agggcgagat ccacaaggcc ctgaagctga aggacggcgg 1441 ccactacctg gtggagttca agtccatcta catggccaag aagcccgtgc agctgcccgg 1501 ctactactac gtggactcca agctggacat cacctcccac aacgaggact acaccatcgt 1561 ggagcagtac gagcgcgccg agggccgcca ccacctgttc ctgtagagtt taaaccgtac 1621 gggaaacggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac 1681 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct 1741 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct 1801 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg 1861 ggaagacaat agcgcggccg ctctagagca tggctacgta gataagtagc atggcgggtt 1921 aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg 1981 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc 2041 ctcagtgagc gagcgagcgc gccagctggc gtaatagcga agaggcccgc accgatcgcc 2101 cttcccaaca gttgcgcagc ctgaatggcg aatggaattc cagacgattg agcgtcaaaa 2161 tgtaggtatt tccatgagcg tttttcctgt tgcaatggct ggcggtaata ttgttctgga 2221 tattaccagc aaggccgata gtttgagttc ttctactcag gcaagtgatg ttattactaa 2281 tcaaagaagt attgcgacaa cggttaattt gcgtgatgga cagactcttt tactcggtgg 2341 cctcactgat tataaaaaca cttctcagga ttctggcgta ccgttcctgt ctaaaatccc 2401 tttaatcggc ctcctgttta gctcccgctc tgattctaac gaggaaagca cgttatacgt 2461 gctcgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 2521 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 2581 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 2641 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 2701 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 2761 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 2821 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 2881 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttaaa 2941 tatttgctta tacaatcttc ctgtttttgg ggcttttctg attatcaacc ggggtacata 3001 tgattgacat gctagtttta cgattaccgt tcatcgattc tcttgtttgc tccagactct 3061 caggcaatga cctgatagcc tttgtagaga cctctcaaaa atagctaccc tctccggcat 3121 gaatttatca gctagaacgg ttgaatatca tattgatggt gatttgactg tctccggcct 3181 ttctcacccg tttgaatctt tacctacaca ttactcaggc attgcattta aaatatatga 3241 gggttctaaa aatttttatc cttgcgttga aataaaggct tctcccgcaa aagtattaca 3301 gggtcataat gtttttggta caaccgattt agctttatgc tctgaggctt tattgcttaa 3361 ttttgctaat tctttgcctt gcctgtatga tttattggat gttggaattc ctgatgcggt 3421 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa 3481 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 3541 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 3601 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 3661 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 3721 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 3781 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 3841 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 3901 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 3961 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 4021 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 4081 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 4141 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 4201 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 4261 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 4321 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 4381 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 4441 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 4501 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 4561 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 4621 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 4681 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 4741 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 4801 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 4861 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 4921 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 4981 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 5041 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 5101 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 5161 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 5221 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 5281 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 5341 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 5401 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 5461 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 5521 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 5581 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 5641 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatg //