LOCUS       Exported                5891 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-E1.1-NRSE-RFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
            Callaway EM
  TITLE     Short Promoters in Viral Vectors Drive Selective Expression in 
            Mammalian Inhibitory Neurons, but do not Restrict Activity to 
            Specific Inhibitory Cell-Types.
  JOURNAL   Front Neural Circuits. 2009  . 3():19.
  PUBMED    19949461
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   7..136
                     /label=AAV2 ITR
     primer_bind     complement(333..349)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        413..451
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          510..985
                     /label=-beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     1067..1083
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1116..1125
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1122..1799
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(1252..1270)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(1302..1322)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1520..1539
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1714..1737
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        complement(1829..1847)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1830..1847)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1867..1884)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1873..1984
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2675..3188
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2762..2781)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2972..2993
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3669..3688)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3788..3810
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3848..3866)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3934..4038
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4039..4899
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4257..4276)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5070..5658
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5559..5578
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5812..5829
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagag gatccttcga aaagcttgta tacggccggc cacgcgtgga tcctcattca
      241 cgcctagaag cagagatcct cattcacgcc tagaagcaga gatcctcatt cacgcctaga
      301 agcagagatc ctcattcacg cctagaagca gagatccact agttctagag cggccgccac
      361 cgacgagcta ggcctgcagg agattaatta attcagcacc acggacagcg ccggtaggcg
      421 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
      481 cgccgagctc ggatcctgag aacttcaggg tgagtctatg ggacccttga tgttttcttt
      541 ccccttcttt tctatggtta agttcatgtc ataggaaggg gagaagtaac agggtacaca
      601 tattgaccaa atcagggtaa ttttgcattt gtaattttaa aaaatgcttt cttcttttaa
      661 tatacttttt tgtttatctt atttctaata ctttccctaa tctctttctt tcagggcaat
      721 aatgatacaa tgtatcatgc ctctttgcac cattctaaag aataacagtg ataatttctg
      781 ggttaaggca atagcaatat ttctgcatat aaatatttct gcatataaat tgtaactgat
      841 gtaagaggtt tcatattgct aatagcagct acaatccagc taccattctg cttttatttt
      901 atggttggga taaggctgga ttattctgag tccaagctag gcccttttgc taatcatgtt
      961 catacctctt atcttcctcc cacagctcct gggcaacgtg ctggtctgtg tgctggccca
     1021 tcactttggc aaagcacgtg aattcgaaga tctggccgcc tcggcctcta gaactagtgg
     1081 atcccccggg ctgcaggaat tcgatgctac cggtcgccac catggcctcc tccgaggacg
     1141 tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt
     1201 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     1261 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttccagt
     1321 acggctccaa ggtgtacgtg aagcaccccg ccgacatccc cgactacaag aagctgtcct
     1381 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     1441 tgacccagga ctcctccctg caggacggct ccttcatcta caaggtgaag ttcatcggcg
     1501 tgaacttccc ctccgacggc cccgtaatgc agaagaagac tatgggctgg gaggcctcca
     1561 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccacaag gccctgaagc
     1621 tgaaggacgg cggccactac ctggtggagt tcaagtccat ctacatggcc aagaagcccg
     1681 tgcagctgcc cggctactac tacgtggact ccaagctgga catcacctcc cacaacgagg
     1741 actacaccat cgtggagcag tacgagcgcg ccgagggccg ccaccacctg ttcctgtaga
     1801 gtttaaaccg tacgggaaac ggccctattc tatagtgtca cctaaatgct agagctcgct
     1861 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     1921 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     1981 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     2041 agggggagga ttgggaagac aatagcgcgg ccgctctaga gcatggctac gtagataagt
     2101 agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct
     2161 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     2221 ttgcccgggc ggcctcagtg agcgagcgag cgcgccagct ggcgtaatag cgaagaggcc
     2281 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa ttccagacga
     2341 ttgagcgtca aaatgtaggt atttccatga gcgtttttcc tgttgcaatg gctggcggta
     2401 atattgttct ggatattacc agcaaggccg atagtttgag ttcttctact caggcaagtg
     2461 atgttattac taatcaaaga agtattgcga caacggttaa tttgcgtgat ggacagactc
     2521 ttttactcgg tggcctcact gattataaaa acacttctca ggattctggc gtaccgttcc
     2581 tgtctaaaat ccctttaatc ggcctcctgt ttagctcccg ctctgattct aacgaggaaa
     2641 gcacgttata cgtgctcgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     2701 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2761 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2821 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2881 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2941 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     3001 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     3061 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     3121 tttacaattt aaatatttgc ttatacaatc ttcctgtttt tggggctttt ctgattatca
     3181 accggggtac atatgattga catgctagtt ttacgattac cgttcatcga ttctcttgtt
     3241 tgctccagac tctcaggcaa tgacctgata gcctttgtag agacctctca aaaatagcta
     3301 ccctctccgg catgaattta tcagctagaa cggttgaata tcatattgat ggtgatttga
     3361 ctgtctccgg cctttctcac ccgtttgaat ctttacctac acattactca ggcattgcat
     3421 ttaaaatata tgagggttct aaaaattttt atccttgcgt tgaaataaag gcttctcccg
     3481 caaaagtatt acagggtcat aatgtttttg gtacaaccga tttagcttta tgctctgagg
     3541 ctttattgct taattttgct aattctttgc cttgcctgta tgatttattg gatgttggaa
     3601 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     3661 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     3721 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     3781 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     3841 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     3901 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3961 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4021 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4081 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4141 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     4201 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     4261 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     4321 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     4381 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     4441 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4501 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4561 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4621 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4681 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4741 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4801 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4861 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4921 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4981 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5041 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5101 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5161 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     5221 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5281 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5341 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5401 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5461 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5521 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5581 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5641 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5701 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5761 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5821 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     5881 attcattaat g
//
