LOCUS Exported 3015 bp ds-DNA linear SYN 03-SEP-2021 DEFINITION Encodes RVD positions 7-9 in TALEN assembly. ACCESSION . VERSION . KEYWORDS pFusX3-23 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3015) AUTHORS Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP, Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker SC TITLE FusX: A rapid one-step TALE assembly system for genome science. JOURNAL Hum Gene Ther. 2016 Feb 8. PUBMED 26854857 REFERENCE 2 (bases 1 to 3015) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 3, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3015 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 204..220 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(295..315) /label=T3 /note="T3 promoter, forward primer" promoter complement(295..313) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(334..350) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(334..350) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(347..369) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 358..374 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(382..412) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 427..448 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." CDS 922..1713 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 1806..2394 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2295..2314 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2548..2565 /label=L4440 /note="L4440 vector, forward primer" terminator 2724..2751 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 2843..2929 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 2993..3009 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 cgtctccatg gcctgacccc ggaccaagtg gtggctatcg agaccggcgc cgctacgggc 61 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggctctt 121 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggaacgcc 181 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatcgactca 241 ctatagggcg aattgggtac cgggcccccc ctcgaggtcc tccagctttt gttcccttta 301 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 361 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 421 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccaccg 481 gtggtctctg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc 541 caggaccatg gcctgcgaga cgtctagacc agccaggaca gaaatgcctc gacttcgctg 601 ctacccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg 661 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg 721 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt 781 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac 841 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc 901 cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta 961 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg 1021 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg 1081 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact 1141 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac 1201 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag 1261 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc 1321 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc 1381 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg 1441 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt 1501 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg 1561 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga 1621 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg 1681 aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc 1741 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 1801 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 1861 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 1921 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 1981 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 2041 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2101 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2161 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc 2221 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 2281 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 2341 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 2401 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 2461 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 2521 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 2581 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 2641 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc 2701 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt 2761 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa 2821 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac 2881 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag 2941 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg 3001 acggccagtc ttaag //