LOCUS Exported 2991 bp ds-DNA linear SYN 13-JUL-2021 DEFINITION Encodes RVD positions 13-15 in TALEN assembly. ACCESSION . VERSION . KEYWORDS pFusB3-41 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2991) AUTHORS Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP, Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker SC TITLE FusX: A rapid one-step TALE assembly system for genome science. JOURNAL Hum Gene Ther. 2016 Feb 8. PUBMED 26854857 REFERENCE 2 (bases 1 to 2991) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2991 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(3..341) /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLDSH HLVRGQAMR" primer_bind 206..222 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 232..251 /label=T7 /note="T7 promoter, forward primer" promoter 232..250 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(298..318) /label=T3 /note="T3 promoter, forward primer" promoter complement(298..316) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(337..353) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(337..353) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(350..372) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 361..377 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(385..415) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 430..451 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." CDS 898..1689 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 1782..2370 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2271..2290 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2524..2541 /label=L4440 /note="L4440 vector, forward primer" terminator 2700..2727 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 2819..2905 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 2969..2985 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 cgtctcatgg cctgaccccg gaccaagtgg tggctatcga gaccggcgcc gctacagggc 61 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct 121 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg 181 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac 241 tcactatagg gcgaattggg taccgggccc cccctcgagg tcctccagct tttgttccct 301 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 361 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 421 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 481 ccggtggtct cgccaggacc atggcctgac cccggaccaa gtggtggcta tcgagacgtc 541 tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc 601 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag 661 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc 721 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag 781 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta 841 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg 901 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag 961 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc 1021 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca 1081 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag 1141 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat 1201 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc 1261 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat 1321 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat 1381 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc 1441 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa 1501 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag 1561 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg 1621 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc 1681 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc 1741 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 1801 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 1861 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 1921 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 1981 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 2041 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 2101 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 2161 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 2221 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 2281 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 2341 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 2401 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 2461 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 2521 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 2581 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 2641 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa 2701 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc 2761 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat 2821 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt 2881 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta 2941 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa g //