LOCUS       Exported                 916 bp ds-DNA     linear   SYN 26-MAY-2021
DEFINITION  UAS transgenesis vector.
ACCESSION   .
VERSION     .
KEYWORDS    pT1UciMP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Horstick EJ, Jordan DC, Bergeron SA, Tabor KM, Serpe M, Feldman B, 
            Burgess HA
  TITLE     Increased functional protein expression using nucleotide sequence 
            features enriched in highly expressed genes in zebrafish.
  JOURNAL   Nucleic Acids Res. 2015 Jan 27. pii: gkv035.
  PUBMED    25628360
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 26, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      148..157
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(742..759)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(742..758)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(751..773)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtgatgactg ttctcacctg gcctgtttgc tttcattatt gatgaagagc tttctagaga
       61 attctgaagc tcgtctttgt cctgaattca tctgtattca tttaaaaaaa aaaattaacc
      121 ttaatttttt tttcttctca gcgtaaagcc accatggaga tctcgagact agtaattaag
      181 tctcagccac cgttaactga acatgtcaaa acctgtggag actgttgaga tttgatgttc
      241 tgaaaagata aagcctataa ataaaatgtt gcccaaattt cctgcctgat gtttttcttt
      301 gtctttgcta catggctttg ctgctcggat cggctcactc tgtgtatgcc acgttcactt
      361 tgtactctcc ttctcacggt aggtttatta tttttagatg tgcagttagt ttctgtgaaa
      421 taacacacca cacactgata ttgtctgtgc attgacttgg tgagtgcaca ttgtttttga
      481 tcttgacata tttatatttg attgatcagg tgaactgtgt gaatctaaag tgctccatac
      541 agatgttctg cattgaaaat attctcattt tattagtgga agtgagtgta tgccagcggc
      601 cgcggtaccc tgcagtctag acatttcagt tgacgaagac aaacaaagtt ctgttgtgac
      661 tatggggggg gggggcgcct ggggatggtc tcgcccgggg agtaattcag ggtagaaccg
      721 ccactgcctt tagcgacatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      781 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      841 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      901 cctgatgcgg tatttt
//
