LOCUS Exported 6748 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expresses VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by the extracellular and transmembrane domain of p75NTR and a restriction site. ACCESSION . VERSION . KEYWORDS P75ECD-VfAU1-LOV_448 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6748) TITLE MYR-VfAU1-LOV_135 JOURNAL Unpublished REFERENCE 2 (bases 1 to 6748) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6748 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 943..952 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2239..2265 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" primer_bind complement(2342..2359) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2348..2572 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 2618..3046 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2705..2724) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2915..2936 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3055..3075) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3060..3389 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3240..3375 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3302..3321 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3456..4250 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3510..3529) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4120..4139 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 4424..4545 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4461..4480) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4515..4534 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4594..4610) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4594..4610) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4607..4629) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4618..4634 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4642..4672) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4687..4708 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4825..4842) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4996..5581) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5076..5095) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5752..6612) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6375..6394 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6613..6717) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaacgg gccctctaga ctcgagcggc cgcggccggc gcgccaccat gggagcaggt 961 gcaactggta gagcaatgga cggacctaga ctcctgctgc tcctcctgct cggagtgtcc 1021 ctgggtggag caaaagaagc atgcccaact ggactctaca cccattctgg agagtgctgc 1081 aaggcctgca atctgggtga aggagtcgct cagccatgtg gagcaaacca gaccgtctgt 1141 gagccttgcc tggattctgt gacattctcc gacgtggtgt ctgccaccga gccttgcaag 1201 ccatgcaccg agtgcgttgg cctgcagtca atgtctgctc cctgcgtgga ggctgacgac 1261 gcagtgtgta gatgcgcata tggctactac caggatgaaa ctactgggag gtgcgaggca 1321 tgtagggtgt gtgaggcagg cagtggtctg gttttcagtt gccaggacaa gcagaacacc 1381 gtctgcgagg agtgtccaga cgggacatac agcgacgaag ccaaccatgt cgatccctgc 1441 ctgccatgta ccgtttgcga agataccgaa cgccagctga gagaatgcac tagatgggct 1501 gacgccgaat gtgaggagat cccaggacgg tggattactc gctctactcc tccagaaggg 1561 tcagatagca ccgctcccag cacccaagaa cccgaagctc ctcccgagca ggatctgatt 1621 gcctccacag ttgctggagt cgtgactacc gtgatgggtt cctcacaacc cgtcgtgacc 1681 agagggacaa ccgacaacct gattccagtg tattgctcta ttctggctgc cgtcgtggtg 1741 ggactggttg catacatagc cttcaagagg gccaccggtg gccctgacta cagtctcgtg 1801 aaggctctgc aaatggcaca acagaatttt gtcattacag acgcctccct cccagacaac 1861 cctatcgtct acgccagtag agggtttctg acactgacag gctattctct cgaccagatc 1921 ctgggcagga actgcaggtt tctgcaaggg ccagaaacag acccaagagc tgtggataag 1981 atcaggaatg ccatcaccaa aggcgttgat accagtgtct gtctgctgaa ttatagacag 2041 gatggcacaa ccttctggaa tctcttcttc gtggctggac tcagagattc taagggcaat 2101 attgtcaact acgtcggagt gcagtcaaag gtgagcgaag attatgccaa gctgctggtc 2161 aacgagcaga acattgagta caaaggtgtg cgcaccagta acatgctgcg cagaaagccc 2221 ggtggatccg gagtcgacta tccgtacgac gtaccagact acgcactcga ctaagaattc 2281 caccacactg gactagtgga tccgagctcg gtaccaagct taagtttaaa ccgctgatca 2341 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 2401 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 2461 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 2521 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 2581 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 2641 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 2701 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 2761 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 2821 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 2881 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 2941 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 3001 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 3061 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 3121 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 3181 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 3241 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 3301 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 3361 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 3421 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 3481 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 3541 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 3601 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt 3661 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 3721 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 3781 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 3841 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 3901 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 3961 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg 4021 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 4081 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 4141 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 4201 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct 4261 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac 4321 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 4381 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc 4441 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 4501 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc 4561 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 4621 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 4681 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 4741 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 4801 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 4861 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 4921 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 4981 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 5041 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 5101 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 5161 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 5221 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 5281 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 5341 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 5401 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 5461 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 5521 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 5581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 5641 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 5701 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 5761 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 5821 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 5881 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 5941 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 6001 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 6061 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 6121 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 6181 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 6241 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 6301 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 6361 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 6421 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 6481 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 6541 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 6601 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 6661 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 6721 atttccccga aaagtgccac ctgacgtc //