LOCUS       Exported               10834 bp ds-DNA     circular SYN 17-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    HA Raptor
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Kim DH, Sarbassov DD, Ali SM, King JE, Latek RR, Erdjument-Bromage 
            H, Tempst P, Sabatini DM
  TITLE     mTOR interacts with raptor to form a nutrient-sensitive complex that
            signals to the cell growth machinery.
  JOURNAL   Cell 2002 Jul 26;110(2):163-75.
  PUBMED    12150925
REFERENCE   2  (bases 1 to 10834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 17, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10834
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(79..98)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      892..1480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1381..1400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1634..1651
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1768..1789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1804..1834
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1842..1858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1847..1869
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1866..1882
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1866..1882
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        1911..2290
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2291..2494
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2445..2465
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2491..2515
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2725..2743
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2725..2742
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2756..2775
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      2860..2869
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2872..2898
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      9031..9040
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    9044..9178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9081..9100)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9135..9154
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        9247..9604
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(9270..9290)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9455..9590
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9517..9536
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(9624..9641)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9624..9640)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9633..9655)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9853..10308
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9940..9959)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10150..10171
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10325..10344)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10444..10466
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10504..10522)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10590..10694
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10695..721
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
       61 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
      121 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
      181 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
      241 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
      301 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
      361 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
      421 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
      481 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
      541 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
      601 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
      661 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
      721 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
      781 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
      841 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      901 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      961 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1021 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1081 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1141 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1201 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1261 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1321 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1381 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1441 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1501 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1561 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     1621 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     1681 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     1741 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     1801 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     1861 tcacacagga aacagctatg acatgattac gaattaattc gagctcgccc gacattgatt
     1921 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1981 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2041 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2101 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2161 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2221 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2281 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2341 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2401 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2461 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2521 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg
     2581 cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg taagtaccgc
     2641 ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca attaatacat
     2701 aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat ccactttgcc
     2761 tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat cgattgaatt
     2821 cccgaaccga cagtcggtct cttcaccaag gccattcgcg ccaccatggg ctacccctat
     2881 gatgtgcctg actacgcaga tctcaatggt ggtggtggtg ggtcgacgat ggagtccgaa
     2941 atgctgcaat cgcctcttct gggcctgggg gaggaagatg aggctgatct tacagactgg
     3001 aacctacctt tggcttttat gaaaaagagg cactgtgaga aaattgaagg ctccaaatcc
     3061 ttagctcaga gctggaggat gaaggatcgg atgaagacag tcagtgttgc cttagttttg
     3121 tgcctgaatg ttggtgtgga ccctcccgat gtggtgaaga ccacgccctg tgcacgcttg
     3181 gaatgctgga tcgatcctct gtcgatgggt cctcagaaag ctctggaaac catcggtgca
     3241 aatttacaga agcagtacga gaactggcag ccaagggccc ggtacaagca gagccttgac
     3301 ccaactgtgg atgaagtcaa gaagctctgc acgtccttac gtcgcaacgc caaggaggag
     3361 cgagtcctct ttcactacaa tggccacggg gtgccccggc ccacagtcaa cggggaggtc
     3421 tgggtcttca acaagaacta cacgcagtac atccctctgt ccatatatga cctgcagacg
     3481 tggatgggca gcccgtcgat cttcgtctac gactgctcca atgctggctt gatcgtcaag
     3541 tccttcaagc agttcgcact acagcgggag caggagctgg aggtagctgc aatcaaccca
     3601 aatcaccctc ttgctcagat gcctttgcct ccgtcgatga aaaactgcat ccagctggca
     3661 gcctgcgagg ccaccgagct gctgcccatg atccccgacc tcccggctga cctattcacc
     3721 tcctgcctca ccacccccat caagatcgcc ctgcgctggt tttgcatgca gaaatgtgtc
     3781 agtctggtgc ctggcgtcac actggatttg atagaaaaga tccctggccg cctgaacgac
     3841 aggaggacgc ccctgggtga actgaactgg atcttcacag ccatcacaga caccatcgcg
     3901 tggaacgtgc tcccccggga tctcttccaa aagctcttca gacaggactt gctggtggct
     3961 agtctgtttc gaaatttttt attggcggaa aggattatga ggtcgtataa ctgcactccc
     4021 gtcagcagcc cgcgtctgcc gcccacgtac atgcacgcca tgtggcaagc ctgggacctg
     4081 gctgttgaca tctgtctgtc tcagctgccg acgatcatcg aggaaggcac tgcgtttcgg
     4141 cacagcccgt tcttcgccga gcagctgacc gcattccagg tgtggctcac catgggcgtg
     4201 gagaaccgaa acccacccga acagctgccc atcgtcctgc aggtgctgtt aagccaagtg
     4261 caccggctga gagcattgga cttgcttgga agatttttgg acctgggtcc ctgggcagtg
     4321 agcctggcct tgtctgtcgg catcttcccc tacgtgctga agctgctcca gagctcggcc
     4381 cgagagctgc ggccacttct cgttttcatc tgggccaaga tcctcgcagt ggacagctcg
     4441 tgccaggcgg acctcgtgaa ggacaacggc cacaagtact tcctgtcggt cctggcggac
     4501 ccctacatgc cagctgaaca ccggaccatg acggctttca ttctcgccgt gatcgtcaac
     4561 agctatcaca cggggcagga agcctgcctt cagggaaacc tcattgccat ctgcctggag
     4621 cagctcaacg acccgcaccc cttgctgcgc cagtgggtgg ccatctgcct cggcaggatc
     4681 tggcagaact tcgactcggc gaggtggtgc ggcgtgaggg acagcgctca tgagaagctc
     4741 tacagcctcc tctccgaccc cattcccgag gtccgctgcg cagcggtctt cgcccttggc
     4801 acgttcgtgg gcaactctgc agagaggacg gaccactcca ccaccatcga ccacaacgtg
     4861 gccatgatgc tggcccagct ggtcagcgac gggagcccca tggtccggaa ggagctggtg
     4921 gtggctctga gtcatcttgt ggttcagtat gaaagcaatt tctgcaccgt ggccctgcag
     4981 ttcatagaag aggaaaagaa ctacgccttg ccttctccag caaccacaga gggagggagt
     5041 ttgaccccag tgcgagacag cccgtgcacc cccagacttc gttctgtgag ctcctatgga
     5101 aacatccgtg ctgtcgccac agccaggagc ctcaacaaat ctttgcagaa cctgagtttg
     5161 acagaggaat ctggtggcgc ggtggcgttc tcccccggaa acctcagcac cagcagcagc
     5221 gccagcagca ccctgggcag ccccgagaat gaggagcata tcctgtcctt cgagaccatc
     5281 gacaagatgc gccgcgccag ctcctactcc tccctcaact ccctcatcgg agtttccttt
     5341 aacagtgttt acactcagat ttggagagtc ctgctgcacc tggctgctga cccctatcca
     5401 gaggtctcgg acgtggccat gaaagtactc aacagcatcg cctacaaggc caccgtgaac
     5461 gcccggccgc agcgcgtcct ggacacctcc tccctcacgc agtcggcccc cgccagcccc
     5521 accaacaagg gcgtgcacat ccaccaggcg gggggctccc ctccggcgtc cagcaccagc
     5581 agctccagcc tgaccaacga tgtggccaag cagccggtca gccgagactt gccttctggc
     5641 cggccgggca ccacaggccc cgctggggcg cagtacaccc ctcactccca ccagttcccc
     5701 cggacacgga agatgttcga caagggccca gagcagactg cggacgacgc ggacgatgct
     5761 gctggacaca aaagtttcat ctccgccacg gtgcagacgg ggttctgcga ctggagcgcc
     5821 cgctattttg cccagcccgt catgaagatc ccagaagagc acgacctgga gagtcagatc
     5881 cgcaaggagc gggagtggcg gttcctgcga aacagccgtg tcaggaggca ggcccagcaa
     5941 gtcattcaga agggcattac gagattggac gaccaaatat ttctgaacag gaaccccggc
     6001 gtcccctctg tggtgaaatt ccaccccttc acgccgtgca tcgctgtagc cgacaaggac
     6061 agcatctgct tttgggactg ggagaaaggg gagaagctgg attatttcca caatgggaac
     6121 cctcggtaca cgagggtcac tgccatggag tatctgaatg gccaggactg ctcgcttctg
     6181 ctgacggcca cagacgatgg tgccatcagg gtctggaaga attttgctga tttggaaaag
     6241 aacccagaga tggtgaccgc gtggcagggg ctctcggaca tgctgccaac gacgcgagga
     6301 gctgggatgg tggtggactg ggagcaggag accggcctcc tcatgagctc aggagacgtg
     6361 cggatcgtcc ggatctggga cacagaccgt gagatgaagg tgcaggacat ccctacgggc
     6421 gcagacagct gtgtgacgag tctgtcctgt gattcccacc gctcactcat cgtggctggc
     6481 ctcggtgacg gctccatccg cgtctacgac agaaggatgg cactcagcga atgccgcgtc
     6541 atgacgtacc gggagcacac agcctgggtg gtgaaggcct ccctgcagaa gcgtcccgac
     6601 ggccacatcg tgagtgtgag cgtcaatgga gatgtgcgca tctttgatcc ccggatgcct
     6661 gagtcggtaa atgtgcttca gatcgtgaag gggctgacgg ccctggacat ccacccccag
     6721 gcggacctga tcgcatgtgg ctccgtcaat cagttcaccg ccatctacaa cagcagcgga
     6781 gagctcatca acaacatcaa gtactacgac ggcttcatgg gccagcgggt cggcgccatc
     6841 agctgcctgg ccttccaccc gcactggcct cacctggccg tgggaagcaa cgactactac
     6901 atctccgtgt actcggtgga gaagcgtgtc agatagcggc gtgacccggg cccaccaggc
     6961 cacggccgcc tgctgtacat agtgaagctg tcactcgccg gggcacgggg cgtcggctgc
     7021 tgcggccccg cagtgtgaac gttggctgct gccttagctg ctgatgacgg caggagggcc
     7081 ctgctactcg cttttgtctg tcttcgctgt cgtgtctgga atgtcaggga aggggagggc
     7141 tcgggttgac ggtggcttcc cactgagcac cagcatccag gtgcaccccc gcggccacgg
     7201 cgcctctgtc cctctcctgt tctgtgtttc tctgagacgc tgaaagggga aacacctcac
     7261 tttatttcca tgtaatcaga gcattagctg cagaaaaacc cccagacaga gccctggcgg
     7321 agaggcaggc gctggggctc ctacgggtcc ctggggcagc tgtccccatc aggccaagag
     7381 cgagcgagag gcgctgcccc agccaggccc accacctctc acagtcagtg cacgcaagca
     7441 gggacatttc ctagccagct gggggacact ggaaattcgg gaaaccaaga gagaggaaga
     7501 aggagacgcc cctccaactg gcgggtgtga aggaagccgc ccaggggtcc gggctgtcct
     7561 tggccgctgg cagcatcact gagcaggaag cgcacagccc accctccccg cacctccagg
     7621 tctctggact ccagttttgg cccctctcac acagagctgt cagcaggggc cgctgtggcg
     7681 gtgcacaggg gaggcaggtc cttggcgagg tagcccctgc cttaatccac ggggctcctt
     7741 tccctccgaa gggctgctct tccccacagg cgcggggaca gcagcccgac ctgtggtctc
     7801 catgcctgtg ccctcacaca ggtgtagcac acgcatgtgc agatggcacc acggccggca
     7861 cctgggggca cacacatgca ggcggcgtgg tctccctgct ctgtccccac acgttcctca
     7921 catacaggca agaggcactg ccgggtcccg gacggctccg ggtgacacca gccccgtctc
     7981 cagccttgag ccgcccatgc tgatgcgacc tcggctgaca gctgggcctg tggtgcagac
     8041 aggagctgtg tggacagtcc cgcccaggag gggccgcagg gcgtgtatga gcagttttgc
     8101 aaacagaaca caaccacaat gatggtattt tgaaaagtgt tctttccgtg ttcgtcggga
     8161 atcaggatta ttgagaggtg aaggagccag gtggcttcat tctggcggtg agaggcccat
     8221 gaccacggga gtgagagctg gtgtggcgag gcccggctct cctgcggtgt ggctggtggc
     8281 ctgccgtggc caagagcatc ttctgggtgg atggaaccct gcctggtcac atttggccag
     8341 agacacacct ggccctcagg gggctgagct ggagactgag ctggggctgg ccgggacgtg
     8401 acaaggcagg acagaggcgg cccctccgct gctccttttt ggaatgcgag ctcccaccag
     8461 aagaaggttc cggcacgaat cccatcccca cgtctgggcc gagaaagcag cccgggtccg
     8521 gaaggtgtag agagtcccgg cctcactcag ctcacagggc gtgccaggcg gcaacaccag
     8581 aatcttccag aagcccagct ccacccgcac acgcagcttc ccatccagtc cttcaactca
     8641 attcttaccc aacacgcgtt tccgtttgtt ttgagacaaa atcaccacct gtcaaaaggc
     8701 aggtggctcc agaggggtca agaccccccc ccccgccccc gctccaccct ggagcccacc
     8761 cccatgggca ctgcgtgccg cctgcacgtg ggctgtcttc acaggtctga tgtgaaaatt
     8821 caatcacgac gttaaccggc tcgagagagc gccggcctag aggctcatta tctatttatt
     8881 ttaccaaacg cgaattgaga cggactttga caaaacacga aatggtaatg tgaagctaag
     8941 agcagagagt gaccaacagt aaacaacacg cgcagactca aaaaaaaaaa aaaaagggcg
     9001 gccgctaagt aagtaaggat ccccagcttg gccgccatgg cccaacttgt ttattgcagc
     9061 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9121 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcgg
     9181 gaattaattc ggcgcagcac catggcctga aataacctct gaaagaggaa cttggttagg
     9241 taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     9301 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     9361 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     9421 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     9481 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9541 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     9601 caaaaagctg ttaacagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     9661 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     9721 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     9781 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     9841 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     9901 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     9961 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
    10021 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
    10081 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
    10141 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
    10201 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
    10261 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
    10321 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
    10381 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
    10441 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
    10501 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
    10561 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    10621 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    10681 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    10741 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    10801 atcagttggg tgcacgagtg ggttacatcg aact
//
