LOCUS       Exported                6162 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Retroviral expression of mouse c-Myc T58A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    pMXs-c-Myc-T58A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Takahashi K, Yamanaka S
  TITLE     Induction of pluripotent stem cells from mouse embryonic and adult 
            fibroblast cultures by defined factors.
  JOURNAL   Cell. 2006 Aug 25. 126(4):663-76.
  PUBMED    16904174
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      247..835
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     736..755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     989..1006
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             1136..1605
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     protein_bind    1922..1946
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3303..3327)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3378..3752
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(3424..3440)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(3762..4178)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(3778..3794)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3810..3832)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    4237..4594
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             4657..5250
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        5280..5384
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5385..83
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5603..5622)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
       61 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
      121 atttgcggcc ggccgcaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
      181 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
      241 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
      301 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
      361 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
      421 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
      481 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
      541 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      601 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      661 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      721 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      781 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      841 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      901 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      961 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca actatggcca
     1081 tttaatgtaa atacttaaga aaaaaaacca aattaatttt gatacatgct gcatgtgaaa
     1141 gacccccgct gacgggtagt caatcactca gaggagaccc tcccaaggaa cagcgagacc
     1201 acaagtcgga tgcaactgca agagggttta ttggatacac gggtacccgg gcgactcagt
     1261 caatcggagg actggcgcgc cgagtgaggg gttgtgggct cttttattga gctcggggag
     1321 cagaagcgcg cgaacagaag cgagaagcga actgattggt tagttcaaat aaggcacagg
     1381 gtcatttcag gtccttgggg caccctggaa acatctgatg gttctctaga aactgctgag
     1441 ggctggaccg catctgggga ccatctgttc ttggccctga gccggggcag gaactgctta
     1501 ccacagatat cctgtttggc ccatattcag ctgttccatc tgttcttggc cctgagccgg
     1561 ggcaggaact gcttaccaca gatatcctgt ttggcccata ttcagctgtt ccatctgttc
     1621 ctgaccttga tctgaacttc tctattctca gttatgtatt tttccatgcc ttgcaaaatg
     1681 gcgttactta agctagcttg ccaaacctac aggtggggtc tttcattccc ccctttttct
     1741 ggagactaaa taaaatcttt tattttatcg tcgaccactg tgctggcggc cgctcgagtt
     1801 taaatagtac ataatggatt tccttacgcg aaatacgggc agacatggcc tgcccggtta
     1861 ttattatttt tgacaccaga ccaactggta atggtagcga ccggcgctca gctggaatat
     1921 caccactttg tacaagaaag ctgggtcggc gcgcccaccc ttttatgcac cagagtttcg
     1981 aagctgttcg agtttgtgtt tcaactgttc tcgtcgtttc ctcaataagt ccttttcaga
     2041 ggtgagcttg tgctcgtctg cttgaatgga caggatgtag gcggtggctt ttttgaggat
     2101 cactaccttg ggggcctttt cgttgttttc caattcaggg atctggtcac gcagggcaaa
     2161 aaagctgcgc ttcagctcgt tcctcctctg acgttccaag acgttgtgtg tccgcctctt
     2221 gtcgttttcc tccgtgtctg aggacctggg gctggagcac ttgcggttgt tgctgatctg
     2281 cttcaggacc ctgccactgt ccaacttggc cctcttggca gctggatagt ccttccttgt
     2341 ggaggggggt gcggcgtagt tgtgctggtg agtggagacg tggcacctct tgaggaccag
     2401 tgggctgtgc ggaggtttgc tgtggcctcg ggatggagat gagcccgact ccgacctctt
     2461 ggcaggggtt tgcctcttct ccacagacac cacatcaatt tcttcctcat cttcttgctc
     2521 ttcttcagag tcgctgctgg tggtgggcgg tgtctcctca tgcagcacta ggggctcagg
     2581 gctggcccgt ggggaggact cggaggacag cagcgagtcc gaggaaggag agaaggccgt
     2641 ggaatcggac gaggtacagg atttgggcga gctgctgtcg ttgagcgggt agggaaagac
     2701 cactgagggg tcaatgcact cggacgcggc ggcggtgagg tcctgcaggt acaggctgga
     2761 ggtggagcag acgctgtgcc cgcgggcggg gctcaggctg gtgctgtctt tgcgcgcagc
     2821 ctggtaggag gccagcttct ccgagaccag cttggcagcg gctgagaaac cgctccacat
     2881 acagtcctgg atgatgatgt tcttgatgaa ggtctcgtcg tcaggatcgc agatgaagct
     2941 ctggttcacc atgtctcctc caagtaactc ggtcatcatc tccagctgat cggcggtgga
     3001 gaagttgcca ccgccgccgt catcgtcttc ccttggggag aaggacgtag cgaccgcaac
     3061 ataggatgga gagcagagcc cggagcggcg gctcggggac aggggcgggg cgggaagcag
     3121 ctcgaatttc ttccagatat cctcactggg cgcgggcggc tgcagctcgc tctgctgttg
     3181 ctggtgatag aaattctctt cctcgtcgca gatgaaatag ggctgtacgg agtcgtagtc
     3241 gaggtcatag ttcctgttgg tgaagttcac gttgaggggc atggtgaagg gggcggccgc
     3301 ggagcctgct tttttgtaca aacttgtgat ttcccgtacc accacactgg gatccttaat
     3361 taactagcta gatccggcag tctagaggat ggtccacccc cggggtcggc agccttcacg
     3421 tgggcggcgt gtatccaagc tgcgatgccg tctactttga gggcggtggg ggtggtcagc
     3481 aggactgtgt aaggtccttt ccagcgaggt tctaggttct tagtctggtg tcggcggacc
     3541 cacactgtgt cgccgactcg gtaagggtga ggtaccaccg gtcggtccag ttgttcttgg
     3601 taggctgccg ccagaggtct ccagacttcg tgctggacta agtagagagc ctgtaagtga
     3661 gcttggagag aggggctgtt agtaactctt gtcatgtcag ggtcagggaa gtttacaagg
     3721 ggcgggggtg ccccatataa gatctcatat ggccatatgg gggcgcctag agaaggagtg
     3781 agggctggat aaagggagga tcgaggcggg gtcgaacgag gaggttcaag ggggagagac
     3841 ggggcggatg gaggaagagg aggcggaggc ttagggtgta caaagggctt gacccaggga
     3901 ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg ggacctggtc tgggtgtcca
     3961 tgcgggccag gtgaaaagac cttgatctta acctgggtga tgaggtctcg gttaaaggtg
     4021 ccgtctcgcg gccatccgac gttaaaggtt ggccattctg cagagcagaa ggtaacccaa
     4081 cgtctcttct tgacatctac cgactggttg tgagcgatcc gctcgacatc tttccagtga
     4141 cctaaggtca aacttaaggg agtggtaaca gtctggccct aattttcaga caaatacaga
     4201 aacacagtca gacagagaca acacagaacg atgctgcagc agacaagacg cgcggcgcgg
     4261 cttcggtccc aaaccgaaag caaaaattca gacggaggcg ggaactgttt taggttctcg
     4321 tctcctacca gaaccacata tccctcctat taggggggtg caccaaagag tccaaaacga
     4381 tcgggatttt tggactcagg tcgggccaca aaaacggccc ccgaagtccc tgggacgtct
     4441 cccagggttg cggccgggtg ttccgaactc gtcagttcca ccacgggtcc gccagataca
     4501 gagctagtta gctaactagt accgacgcag gcgcataaaa tcagtcatag acactagaca
     4561 atcggacaga cacagataag ttgctggcca gcttacctcc cggtggtggg tcggtggtcc
     4621 ctgggcaggg gtctcccgat cccggacgag cccccaaatg aaagaccccc gctgacgggt
     4681 agtcaatcac tcagaggaga ccctcccaag gaacagcgag accacaagtc ggatgcaact
     4741 gcaagagggt ttattggata cacgggtacc cgggcgactc agtcaatcgg aggactggcg
     4801 ccccgagtga ggggttgtgg gctcttttat tgagctcggg gagcagaagc gcgcgaacag
     4861 aagcgagaag cgaactgatt ggttagttca aataaggcac agggtcattt caggtccttg
     4921 gggcaccctg gaaacatctg atggttctct agaaactgct gagggctgga ccgcatctgg
     4981 ggaccatctg ttcttggccc tgagccgggg caggaactgc ttaccacaga tatcctgttt
     5041 ggcccatatt cagctgttcc atctgttctt ggccctgagc cggggcagga actgcttacc
     5101 acagatatcc tgtttggccc atattcagct gttccatctg ttcctgacct tgatctgaac
     5161 ttttctattc tcagttatgt atttttccat gccttgcaaa atggcgttac ttaagctagc
     5221 ttgccaaacc tacaggtggg gtctttcatt atgcaggtgg cacttttcgg ggaaatgtgc
     5281 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5341 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5401 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5461 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5521 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5581 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5641 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5701 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5761 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5821 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5881 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5941 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6001 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6061 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6121 cactggggcc agatggtaag ccctcccgta tcgtagttat ct
//
