LOCUS       Exported                5183 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Empty backbone for retroviral gene expression. Select recipient 
            cells using GFP..
ACCESSION   .
VERSION     .
KEYWORDS    pBABE GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5183)
  TITLE     Viral vectors.
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    57..256
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             457..873
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     803..825
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     841..857
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(931..951)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        936..1265
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1116..1251
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1178..1197
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(1274..1290)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1275..1291)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             1296..2015
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
     LTR             2192..2661
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2791..2808)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2962..3550)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3042..3061)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3710..4570)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4333..4352
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4571..4675)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             4706..5175
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggcaagc
       61 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
      121 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      181 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
      241 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
      301 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
      361 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
      421 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
      481 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
      541 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
      601 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
      661 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
      721 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
      781 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
      841 ctttatccag ccctcactcc ttctctaggc gccggccgga tcccagtgtg gtggtacgta
      901 ggaattcgcc agcacagtgg tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt
      961 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     1021 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     1081 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     1141 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     1201 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     1261 gcaaaaagct tatcgatacc gtcgacctcg agaccatggt gagcaagcag atcctgaaga
     1321 acaccggcct gcaggagatc atgagcttca aggtgaacct ggagggcgtg gtgaacaacc
     1381 acgtgttcac catggagggc tgcggcaagg gcaacatcct gttcggcaac cagctggtgc
     1441 agatccgcgt gaccaagggc gcccccctgc ccttcgcctt cgacatcctg agccccgcct
     1501 tccagtacgg caaccgcacc ttcaccaagt accccgagga catcagcgac ttcttcatcc
     1561 agagcttccc cgccggcttc gtgtacgagc gcaccctgcg ctacgaggac ggcggcctgg
     1621 tggagatccg cagcgacatc aacctgatcg aggagatgtt cgtgtaccgc gtggagtaca
     1681 agggccgcaa cttccccaac gacggccccg tgatgaagaa gaccatcacc ggcctgcagc
     1741 ccagcttcga ggtggtgtac atgaacgacg gcgtgctggt gggccaggtg atcctggtgt
     1801 accgcctgaa cagcggcaag ttctacagct gccacatgcg caccctgatg aagagcaagg
     1861 gcgtggtgaa ggacttcccc gagtaccact tcatccagca ccgcctggag aagacctacg
     1921 tggaggacgg cggcttcgtg gagcagcacg agaccgccat cgcccagctg accagcctgg
     1981 gcaagcccct gggcagcctg cacgagtggg tgtaataggg taccttcgat aaaataaaag
     2041 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     2101 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag
     2161 ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     2221 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca
     2281 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
     2341 cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
     2401 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
     2461 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc
     2521 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca
     2581 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
     2641 cccgtcagcg ggggtctttc acatgcagca tgtatcaaaa ttaatttggt tttttttctt
     2701 aagtatttac attaaatggc catagttgca ttaatgaatc ggccaacgcg cggggagagg
     2761 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2821 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2881 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2941 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3001 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3061 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3121 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3181 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3241 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3301 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3361 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     3421 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3481 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3541 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3601 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3661 gcggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3721 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3781 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3841 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3901 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3961 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4021 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4081 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4141 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4201 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4261 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4321 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4381 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4441 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4501 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4561 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4621 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4681 ttccccgaaa agtgccacct gcagcctgaa tatgggccaa acaggatatc tgtggtaagc
     4741 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg
     4801 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     4861 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     4921 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     4981 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt
     5041 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     5101 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     5161 agcgggggtc tttcatttgg ggg
//
