LOCUS       Exported                5757 bp ds-DNA     circular SYN 23-SEP-2022
DEFINITION  Luciferase reporter containing three copies of vitellogenin Estrogen
            Response Element .
ACCESSION   .
VERSION     .
KEYWORDS    3X ERE TATA luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Hall JM, McDonnell DP
  TITLE     The estrogen receptor beta-isoform (ERbeta) of the human estrogen 
            receptor modulates ERalpha transcriptional activity and is a key 
            regulator of the cellular response to estrogens and antiestrogens.
  JOURNAL   Endocrinology. 1999 Dec . 140(12):5566-78.
  PUBMED    10579320
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 23, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     intron          387..452
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             582..602
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1027..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1303..1320)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1474..2062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1554..1573)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2233..3093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2856..2875
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3094..3198)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3225..3680
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3312..3331)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3522..3543
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    3870..3991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             4251..146
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(4317..4335)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac
       61 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca
      121 agaagggcgg aaagtccaaa ttgtaaaatg taactgtatt cagcgatgac gaaattctta
      181 gctattgtaa tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg
      241 gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga tgaatggcag
      301 aaattcgccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
      361 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
      421 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
      481 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
      541 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
      601 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
      661 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
      721 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
      781 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
      841 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
      901 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
      961 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     1021 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1081 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1141 gtatcttatc atgtctggat ccgtcgaccg atgcccttga gagccttcaa cccagtcagc
     1201 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     1261 atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct
     1321 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1381 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1441 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1501 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1561 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1621 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1681 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1741 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1801 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1861 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1921 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1981 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2041 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2101 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2161 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2221 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2281 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2341 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2401 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2461 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2521 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2581 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2641 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2701 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2761 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2821 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2881 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2941 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3001 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3061 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3121 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3181 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
     3241 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3301 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3361 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3421 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     3481 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     3541 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     3601 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     3661 aatattaacg cttacaattt gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3721 atcggtgcgg gcctcttcgc tattacgcca gcccaagcta ccatgataag taagtaatat
     3781 taaggtacgt ggaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     3841 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     3901 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3961 gtggtttgtc caaactcatc aatgtatctt atggtactgt aactgagcta acataacccg
     4021 ggaggtaccg agctcttacg cgtgctagct cgagatctag gtcacagtga cctgcggatc
     4081 cgcaggtcac tgtgacctag atccgcaggt cactgtgacc tagatctgat atcatcgatg
     4141 aattcgggct ataaaagggg gtggggggag ctcggccctc attctggaga cggatcctct
     4201 agagtcgacc tgcaggcatg caagcttggc attccggtac tgttggtaaa atggaagacg
     4261 ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag
     4321 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
     4381 atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg
     4441 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
     4501 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
     4561 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta
     4621 ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac
     4681 caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
     4741 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag
     4801 agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga tctactgggt
     4861 tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag
     4921 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
     4981 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
     5041 taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa
     5101 gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg attgacaaat
     5161 acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg
     5221 gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg
     5281 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta
     5341 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg
     5401 ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt tatgtaaaca
     5461 atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag
     5521 cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat
     5581 acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa caccccaaca
     5641 tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg
     5701 ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcg
//
