LOCUS       Exported                 854 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast 
            resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE).
ACCESSION   .
VERSION     .
KEYWORDS    lenti dCAS-VP64_Blast
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena 
            C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
  TITLE     Genome-scale transcriptional activation by an engineered CRISPR-Cas9
            complex.
  JOURNAL   Nature. 2014 Dec 10. doi: 10.1038/nature14136.
  PUBMED    25494202
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 gttcgctcag gccgcctctc tcggccttgg tcagattgtc gaactttctc tgggtaatca
       61 gcttggcgtt cagcagctgc cgccagtagt tcttcatctt cttcacgacc tcttcggagg
      121 gcacgttgtc gctcttgccc cgggccttgt cgcttctggt cagcaccttg ttgtcgatgg
      181 agtcgtcctt cagaaagctc tgaggcacga tgtggtccac atcgtagtcg gacagccggt
      241 tgatgtccag ttcctggtcc acgtacatat cccgcccatt ctgcaggtag tacaggtaca
      301 gcttctcgtt ctgcagctgg gtgttttcca cggggtgttc tttcaggatc tggctgccca
      361 gctctttgat gccctcttcg atccgcttca ttctctcgcg gctgttcttc tgtcccttct
      421 gggtggtctg gttctctctg gccatttcga tcacgatgtt ctcgggcttg tgccggccca
      481 tcactttcac gagctcgtcc accaccttca ctgtctgcag gatgcccttc ttaatggcgg
      541 ggctgccggc cagattggca atgtgctcgt gcaggctatc gccctggccg gacacctggg
      601 ctttctggat gtcctcttta aaggtcaggc tgtcgtcgtg gatcagctgc atgaagtttc
      661 tgttggcgaa gccgtcggac ttcaggaaat ccaggattgt cttgccggac tgcttgtccc
      721 ggatgccgtt gatcagcttc cggctcagcc tgccccagcc ggtgtatctc cgccgcttca
      781 gctgcttcat cactttgtcg tcgaacaggt gggcataggt tttcagccgt tcctcgatca
      841 tctctctgtc ctca
//
