LOCUS       Exported                 958 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  A single vector AAV-Cas9 system containing Cas9 from Staphylococcus 
            aureus (SaCas9) and its sgRNA..
ACCESSION   .
VERSION     .
KEYWORDS    pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B,
            Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F
  TITLE     In vivo genome editing using Staphylococcus aureus Cas9.
  JOURNAL   Nature. 2015 Apr 1. doi: 10.1038/nature14299.
  PUBMED    25830891
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 tggtcttgct gattctgccc ttgcccttgg ccagattcag gatgtgcttc ttgaaggttt
       61 cgtagctgat cttgctgtcg ctgctgctca ggtactggaa tggggtccgg ttgcccttct
      121 tgctgttttc ttcctgcttc acgagcacct tgttgttgaa gctgttgtcg aaggacacgc
      181 ttctggggat gatgtggtcc acctcatagt tgaaggggtt gttcagcaga tcttccagag
      241 ggatggcttc caggctgtac aggcacttgc cttcctgcat gtcgtgcagc ttgatcttct
      301 cgatcaggta cttggcgttc tctttgccgg tggtccggat gatttcctcg atccgctcgt
      361 tggtctgccg gttccgcttc tgcatctcgt tgatcatttt ctgggcgtcc ttggagttct
      421 tctcgcgggc cagctcgata atgatgtcgt tgggcaggcc gtacttcttg atgatggcgt
      481 tgatcacttt gatgctctgg atgaagcttc tcttcacgac ggggctcagg atgaagtcgt
      541 ccaccagggt ggtggggatc tctttctgct gggacaggtc caccttcttg ggcaccagct
      601 tcagccggtt gaagatagcg atctggttgt cgttggtgtg ccacagctcg tccaggatca
      661 ggttgatggc cttcaggctc aggttgtggg tgccggtata gcccttcaga ttagagatct
      721 gctcgatctc ttcctgggtc agctcggagt tcagattggt cagttcttcc tggatgtcct
      781 cgctgctctg gtagatggtc aggatcttgg caatctgatc cagcagctcg gcgttctcaa
      841 taatctcttt ccgggcggta atgtccttga tgtcgtggta caccttcagg ttggtgaact
      901 cgggcttgcc ggtgctggtc actctgtagc ccttaatatc ctcttcgttc acgaggat
//
