LOCUS       Exported                1018 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  A single vector AAV-Cas9 system containing Cas9 from Staphylococcus 
            aureus (SaCas9) and its sgRNA..
ACCESSION   .
VERSION     .
KEYWORDS    pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B,
            Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F
  TITLE     In vivo genome editing using Staphylococcus aureus Cas9.
  JOURNAL   Nature. 2015 Apr 1. doi: 10.1038/nature14299.
  PUBMED    25830891
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(10..51)
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             complement(52..78)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(85..132)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gaattcttac tttcccatca acctgggtcc aagctcgttg gcgaagccgc tagcgtaatc
       61 tggaacatcg tatgggtagg atcccttttt cttttttgcc tggccggcct ttttcgtggc
      121 cgccggcctt ttgccctttt tgatgatctg agggtgcttc ttagatttca cttcatacag
      181 gttgcccaga atgtctgtgc tgtacttctt aatgctctgg gtcttggagg cgattgtctt
      241 aatgatcctg gggggcctct tgtcgttcat gttttccagg tactcgcggt aggtgatgtc
      301 gatcatgttc acttcgatcc ggttcagcag gtcgttgttc acgccgatca ctctatacag
      361 ctcgccgttg atcttgatca gatcgttgtt gtagaaggag gcgataaact cggcctggtt
      421 gctgatcttc ttcagcttct tagcttcctc atagcacttg ctattcactt cgtagtagtt
      481 ttcttttttg atcacatcca gattcttcac ggtcacgaac ttgtacacgc cattgtccag
      541 gtacacgtcg aatctgtagg gcttcaggga cagcttcacg accttgtttc tgctgttggg
      601 gtagtcgtcg gtgatgtcca gatgggcgtt cagtttgttg ccgtaatact taatcttctt
      661 gatcacgggg ccgttgtcct ttttggagta cttggtcagg tagttcccgg tttcctcgta
      721 gtacttgtac aggggattct tctcgtcgcc gtactgttcc ataatcagct tcagtttctg
      781 gtaggtctgg gggtcgtggt ggtacatcag cagcttttcg gggctcttgt tgatcagctt
      841 tttcagcttg tcattgtcct tgtcgtacag gccgttcaga ttgttcacga tcagggtgtt
      901 gcccttgtcg tccttccggg tggagtacag ggtgtcgtta atcagctctc tattaggctt
      961 cttgtccacc cggtggctgt acttgtagtc cttgaagtcc ttaatgtgct tgatctgg
//
