LOCUS Exported 692 bp ds-DNA linear SYN 09-JUN-2021 DEFINITION aTc inducible gRNA expression vector. ACCESSION . VERSION . KEYWORDS pRPR1(1xTetO)_gRNA_handle_RPR1t SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 692) AUTHORS Farzadfard F, Perli SD, Lu TK TITLE Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. JOURNAL ACS Synth Biol. 2013 Sep 11. PUBMED 23977949 REFERENCE 2 (bases 1 to 692) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 9, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..692 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 383..445 /label=RPR1 terminator /note="transcription terminator for the S. cerevisiae RNase P RNA gene" misc_RNA complement(458..533) /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" protein_bind 625..643 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" ORIGIN 1 gggtaccaaa gaacaacaag agttccaaaa agtagtggaa caaaagcaaa tgaaggattt 61 catgcgtttg tactctaatc tggtagaaag atgtttcaca gactgtgtca atgacttcac 121 aacatcaaag ctaaccaata aggaacaaac atgcatcatg aagtgctcag aaaagttctt 181 gaagcatagc gaacgtgtag ggcagcgttt ccaagaacaa aacgctgcct tgggacaagg 241 cttgggccga taaggtgtac tggcgtatat atatctaatt atgtatctct ggtgtagccc 301 atttttagca tgtaaatata aagagaaacc atatctaatc taaccaaatc caaacaaaat 361 tcaatagtta ctatcgcttt tttctttctg tatcgcaaat aagtgaaaat taaaaaagaa 421 agattaaatt ggaagttgga tatggctcga gaaaaaagca ccgactcggt gccacttttt 481 caagttgata acggactagc cttattttaa cttgctattt ctagctctaa aacactgtat 541 gtacatacag taaaagcttc tgccaatcgc agctcccaga gtttcgttcc cagccgcgag 601 caccacagcg taccatgtgc gccatctcta tcactgatag ggacaatcga aacgaaaaga 661 aacagactat aggggagtat agagacagcc gg //