LOCUS       Exported               12857 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expression of Spectrin-cpstFRET to measure mechanical stress and 
            report multidimensional stress fields .
ACCESSION   .
VERSION     .
KEYWORDS    Spectrin-M-cpstFRET
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12857)
  AUTHORS   Meng F, Sachs F
  TITLE     Orientation-based FRET sensor for real-time imaging of cellular 
            forces.
  JOURNAL   J Cell Sci. 2012 Feb 1;125(Pt 3):743-50. doi: 10.1242/jcs.093104.
  PUBMED    22389408
REFERENCE   2  (bases 1 to 12857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12857
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3912..4427
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIE"
     regulatory      8892..8901
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    9645..9766
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9682..9701)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9736..9755
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9773..10228)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9910..9931)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10122..10141
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10255..10359
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10361..10718
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(10384..10404)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10569..10704
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10631..10650
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             10753..11547
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10807..10826)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11417..11436
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11735..11754)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11779..11826
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      12155..12743
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12644..12663
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtatgga cccaagtggg gtcaaagtgc tggaaacagc agaggacatc caggagaggc
      661 ggcagcaggt cctagaccga taccaccgct tcaaggaact ctcaaccctt aggcgtcaga
      721 agctggaaga ttcctatcga ttccagttct ttcaaagaga tgctgaagag ctggagaaat
      781 ggatacagga aaaacttcag attgcatctg atgagaatta taaagaccca accaacttgc
      841 agggaaagct tcagaagcat caagcatttg aagctgaagt gcaggccaac tcaggagcca
      901 ttgttaagct ggatgaaact ggaaacctga tgatctcaga agggcatttt gcatctgaaa
      961 ccatacggac ccgtttgatg gagctgcacc gccagtggga attacttttg gagaagatgc
     1021 gagaaaaagg aatcaaactg ctgcaggccc agaagttggt gcagtactta cgagaatgtg
     1081 aggacgtgat ggactggatc aatgacaagg aagcaattgt tacttctgaa gagctgggcc
     1141 aggatctgga gcatgtagag gttttacaga agaaatttga agagtttcaa acagatatgg
     1201 ctgctcatga agaaagagtt aatgaagtga accagtttgc tgccaaactc atacaggagc
     1261 agcaccctga ggaggaactg atcaagacta agcaggatga agtcaatgca gcctggcagc
     1321 ggctgaaggg cctggctctg cagaggcagg ggaagctctt tggggcagca gaagttcagc
     1381 gctttaacag ggatgtggat gagactatca gttggattaa ggaaaaggag cagttaatgg
     1441 cctctgatga ttttggccga gacctggcaa gtgttcaggc tctgcttcgg aagcacgagg
     1501 gtctggagag agatcttgct gctctagaag acaaggtcaa agccctgtgt gctgaggctg
     1561 accgcctgca acagtcccac cctctgagtg caacacagat tcaagtgaag cgagaggaac
     1621 tgattacaaa ctgggagcag atccgcacct tggcggcaga gagacatgca cggctcaatg
     1681 attcatacag gcttcaacgc ttccttgctg acttccgtga cctcaccagc tgggtgactg
     1741 agatgaaagc cctcatcaat gcagatgagc ttgccagtga tgtggctggg gctgaagccc
     1801 tgctagatag acaccaagag cacaagggtg aaattgatgc ccatgaagac agcttcaaat
     1861 ctgcagatga atctggacag gcactgcttg ctgctggtca ttatgcctca gatgaagtga
     1921 gggagaagct gaccgtcctt tccgaggaga gagcggcgct gctggagctg tgggagctgc
     1981 gcaggcagca gtacgagcag tgcatggacc tgcagctctt ctaccgggac actgagcagg
     2041 tggacaactg gatgagcaag caggaggcgt tcctgttgaa tgaagacttg ggagattcct
     2101 tggatagtgt ggaagcgctt cttaagaagc acgaagactt tgagaaatcc cttagtgccc
     2161 aggaggaaaa gattacagca ttagatgaat ttgcaaccaa gctaattcag aacaaccact
     2221 atgcaatgga agatgtggcc actcgccgag atgctctgtt gagccgccgc aatgcccttc
     2281 acgagagagc catgcgtcgc cgggcccagc tagccgattc tttccatctg cagcagtttt
     2341 tccgtgattc tgatgagctc aagagttggg tcaatgagaa gatgaaaact gccacagatg
     2401 aagcttataa agatccatcc aacctacaag gaaaagtaca gaagcatcag gcttttgagg
     2461 ctgagctctc agcaaaccag agccgaattg atgccttgga gaaagctggc caaaagctga
     2521 ttgatgtcaa ccactatgcc aaggatgaag tggcagctcg tatgaatgag gtgatcagtt
     2581 tgtggaagaa actgctagag gccactgaac tgaaaggaat aaagcttcgt gaagccaacc
     2641 agcaacagca atttaatcgc aatgttgagg atattgaatt gtggctatat gaagtagaag
     2701 gtcacttggc ttcggatgat tacggcaaag atcttaccaa tgtgcagaac ctccagaaga
     2761 aacatgccct gctagaggca gatgtggctg ctcaccagga ccgaattgat ggcatcacca
     2821 ttcaggcccg ccagttccaa gatgctggcc attttgatgc agaaaacatc aagaagaaac
     2881 aggaagccct cgtggctcgc tatgaggcac tcaaggagcc catggttgcc cggaagcaga
     2941 agctggccga ttctctgcgg ttgcagcagc tcttccggga tgttgaggat gaggagacgt
     3001 ggattcgaga gaaagagccc attgccgcat ctaccaacag aggtaaggat ttaattgggg
     3061 tccagaatct gctaaagaaa catcaagcct tacaagcaga aattgctgga catgaaccac
     3121 gcatcaaagc agttacacag aaggggaatg ccatggtgga ggaaggccat tttgctgcag
     3181 aggatgtgaa ggccaagctt cacgagctga accaaaagtg ggaggcactg aaagccaaag
     3241 cttcccagcg tcggcaggac ctggaggact ctctgcaggc ccagcagtac tttgctgatg
     3301 ctaacgaggc tgaatcctgg atgcgggaga aggaacccat tgtgggcagc actgactatg
     3361 gcaaggacga agactctgct gaggctctac tgaagaaaca cgaagctttg atgtcagatc
     3421 tcagtgccta cggcagcagc atccaggctt tgcgagaaca agcacagtcc tgccggcaac
     3481 aagtggcccc cacggatgat gagactggga aggagctggt cttggctctc tacgactatc
     3541 aggagaagag tccccgagag gtcaccatga agaagggaga tatccttacc ttactcaaca
     3601 gcaccaacaa ggattggtgg aaagtggaag tgaacgatcg tcagggtttt gtgccggctg
     3661 cgtacgtgaa ggtcgacgga cccggggtac caatgggcgg cgtgcagctc gccgaccact
     3721 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     3781 gctaccagtc caaactgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     3841 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag gaattcggcg
     3901 gcggtggcgg ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     3961 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     4021 ccacctacgg caagctgacc ctgaagctga tctgcaccac cggcaagctg cccgtgccct
     4081 ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg cttcgcccgc taccccgacc
     4141 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     4201 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     4261 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     4321 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacc gccgacaagc
     4381 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcagat
     4441 ctatgggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     4501 ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca
     4561 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     4621 gcatggacga gctgtacaag gaattcggcg gcggtggcgg ggtgagcaag ggcgaggagc
     4681 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     4741 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     4801 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg
     4861 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     4921 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     4981 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     5041 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aacgccatca
     5101 gcgacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     5161 tccgccacaa catcgaggac gagctcgctc tggtgccacg cagcggccgc aaattggacc
     5221 ccgcccagtc agcctcccgg gagaatctcc tggaggagca aggcagcata gcactgcggc
     5281 aggagcagat tgacaatcaa tatcattctc tgctggaact gggtgagaag cgtaaaggca
     5341 tgttggagaa gagttgcaag aagtttatgt tgttccgtga agcgaatgaa ctacagcaat
     5401 ggatcaatga gaaggaagcc gctctgacaa gtgaggaggt cggagcagac ttggagcagg
     5461 ttgaggtgct ccagaagaag tttgatgact tccagaagga cctgaaggcc aatgagtcac
     5521 ggttgaagga cattaacaag gtagctgaag acctggagtc tgaaggtctt atggcagagg
     5581 aggtgcaggc tgtgcaacaa caggaagtgt atggcatgat gcccagggat gaaactgatt
     5641 ccaagacagc ctccccgtgg aagtctgctc gtctgatggt tcacaccgtg gccaccttta
     5701 attccatcaa ggagctgaat gagcgctggc ggtccctaca gcagctggcc gaggaacgga
     5761 gccagctctt gggcagcgcc catgaagtac agaggttcca cagagatgct gatgaaacca
     5821 aagaatggat tgaagagaag aatcaagctc taaacacaga caattatgga catgatctcg
     5881 ccagtgtcca ggccctgcaa cgcaagcatg agggcttcga gagggacctt gcggctctcg
     5941 gtgacaaggt aaactccctt ggtgaaacag cagagcgcct gatccagtcc catcccgagt
     6001 cagcagaaga cctgcaggaa aagtgcacag agttaaacca ggcctggagc agcctgggga
     6061 aacgtgcaga tcagcgcaag gcaaagttgg gtgactccca cgacctgcag cgcttcctta
     6121 gcgatttccg ggacctcatg tcttggatca atggaatacg ggggttggtg tcctcagatg
     6181 agctagccaa ggatgtcacc ggagctgagg cattgctgga gcgacaccag gaacaccgga
     6241 cagaaatcga tgccagggct ggcactttcc aggcatttga gcagtttgga cagcagctgt
     6301 tggctcacgg acactatgcc agccctgaga tcaagcagaa acttgatatt cttgaccagg
     6361 agcgtgcaga cctggagaag gcctgggttc agcgcaggat gatgctggat cagtgccttg
     6421 aactgcagct gttccatcgg gactgtgagc aagctgagaa ctggatggct gcccgggagg
     6481 ccttcttgaa taccgaagac aaaggagact cactggacag cgtagaggct ctgatcaaaa
     6541 aacatgaaga ctttgacaaa gcgattaacg tccaggaaga gaagattgct gctctgcagg
     6601 cctttgccga ccagctcatc gctgccggcc attatgccaa gggagacatt tctagccggc
     6661 gcaatgaggt cttggacagg tggcgacgtc tgaaagccca gatgattgag aaaaggtcaa
     6721 agctaggaga atctcaaacc ctccaacagt tcagccggga tgtggatgag attgaggctt
     6781 ggatcagtga aaaattgcaa acagcgagtg atgagtcgta caaggatccc accaacatcc
     6841 agagcaagca ccagaagcac caggcttttg aagcagagct gcatgccaac gctgaccgga
     6901 tccgtggggt tatcgacatg ggcaactccc tcattgaacg tggagcctgt gccggcagtg
     6961 aggatgctgt caaggcccgc ctggctgcct tagctgacca gtggcagttc ttggtgcaaa
     7021 agtcagcgga aaagagccag aaactgaaag aagccaacaa gcagcagaac ttcaacacag
     7081 ggatcaagga ctttgacttc tggctgtctg aggtggaggc cctgctggca tccgaagatt
     7141 atggcaaaga cctggcttct gtgaacaacc tgctgaaaaa gcatcaactg ctggaagcag
     7201 atatatctgc ccatgaggat cgcctgaagg acctgaacag ccaggcagac agcctgatga
     7261 ccagcagtgc cttcgacacc tcccaagtaa aggacaagag ggacaccatc aacgggcgct
     7321 tccagaagat caagagcatg gcggcctccc ggcgagccaa gctgaatgaa tcccatcgcc
     7381 tgcaccagtt cttccgggac atggatgacg aggagtcctg gatcaaggag aagaagctgc
     7441 tggtgggctc agaggactac ggccgggacc taactggcgt gcagaacctg aggaagaagc
     7501 acaagcggct ggaagcagaa ctggctgcgc atgagccggc tattcagggt gtcctggaca
     7561 ctggcaagaa gctgtccgat gacaacacca tcgggaaaga ggagatccag cagcggctgg
     7621 cgcagtttgt ggagcactgg aaagagctga agcagctggc agctgcccgg ggtcagcggc
     7681 tggaagagtc cttggaatat cagcagtttg tagccaatgt ggaagaggaa gaagcctgga
     7741 tcaatgagaa aatgaccctg gtggccagcg aagattatgg cgacactctt gccgccatcc
     7801 agggcttact gaagaaacat gaagcttttg agacagactt caccgtccac aaggatcgcg
     7861 tgaatgatgt ctgcaccaat ggacaagacc tcattaagaa gaacaatcac catgaggaga
     7921 acatctcttc aaagatgaag ggcctgaacg ggaaagtgtc agacctggag aaagctgcag
     7981 cccagagaaa ggcgaagctg gatgagaact cggccttcct tcagttcaac tggaaggcgg
     8041 acgtggtgga gtcctggatc ggtgaaaagg agaacagctt gaagacagat gattatggcc
     8101 gagacctgtc ttctgtgcag acgctcctca ccaaacagga aacttttgac gctgggctgc
     8161 aggccttcca gcaggaaggc attgccaaca tcactgccct caaagatcag cttctcgccg
     8221 ccaaacacgt tcagtccaag gccatcgagg cccggcacgc ctccctcatg aagaggtgga
     8281 gccagcttct ggccaactca gccgcccgca agaagaagct tctggaggct cagagtcact
     8341 tccgcaaggt ggaggacctc ttcctgacct tcgccaaaaa ggcttctgcc ttcaacagct
     8401 ggtttgaaaa tgcagaggag gacttaacag accccgtgcg ctgcaactcc ttggaagaaa
     8461 tcaaagcttt gcgcgaggcc cacgacgcct tccgctcctc cctcagctct gcccaggctg
     8521 acttcaacca gctggccgag ctggaccgcc agatcaagag cttccgcgta gcctccaacc
     8581 cctacacctg gtttaccatg gaggccctgg aggagacctg gaggaaccta cagaaaatca
     8641 tcaaggagag ggagctggag ctgcagaagg aacagcggcg gcaggaggag aacgacaagc
     8701 tgcgccagga gtttgcccag cacgccaacg ccttccacca gtggatccaa gagaccagga
     8761 catacctcct cgatgggtcc tgtatggtgg aagagtcggg gaccctcgaa tcccagcttg
     8821 aagctaccaa acgcaagcac caggaaatcc gagccatgag aagtcagctc aaaaagatcg
     8881 aggacctggg ggccgccatg gaggaggccc tcatcctgga caacaagtac acggagcaca
     8941 gcaccgtggg cctcgcccag cagtgggacc agctggacca gctgggcatg cgcatgcagc
     9001 acaacctgga gcagcagatc caggccagga acacaacagg tgtgactgag gaggccctca
     9061 aagaattcag catgatgttt aaacactttg acaaggacaa gtctggcagg ctgaaccatc
     9121 aggagttcaa atcttgcctg cgctccctgg gctatgacct gcccatggtg gaggaagggg
     9181 aacctgaccc tgagttcgag gcaatcctgg acacggtgga tccgaacaga gatggccatg
     9241 tctccttgca agaatacatg gctttcatga tcagccgcga aactgagaac gtcaagtcca
     9301 gcgaggagat tgagagcgcc ttccgggccc tcagctcaga gggaaagcct tacgtgacca
     9361 aggaggagct ctaccagaac ctgacccggg aacaagccga ctactgcgtc tcccacatga
     9421 agccctacgt ggacggcaag ggccgcgagc tccccaccgc gttcgactac gtggagttca
     9481 cccgctcgct tttcgtgaac ggtaccgcgg gcccgggatc caccggatct agataactga
     9541 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     9601 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     9661 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9721 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta
     9781 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     9841 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     9901 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     9961 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
    10021 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
    10081 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
    10141 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
    10201 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
    10261 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
    10321 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
    10381 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
    10441 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
    10501 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
    10561 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
    10621 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    10681 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
    10741 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
    10801 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
    10861 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
    10921 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
    10981 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
    11041 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
    11101 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
    11161 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
    11221 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
    11281 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
    11341 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
    11401 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
    11461 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
    11521 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
    11581 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
    11641 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
    11701 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
    11761 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
    11821 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
    11881 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
    11941 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
    12001 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    12061 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    12121 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    12181 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    12241 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    12301 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    12361 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    12421 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    12481 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    12541 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    12601 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    12661 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    12721 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    12781 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    12841 gtattaccgc catgcat
//
