LOCUS Exported 680 bp ds-DNA linear SYN 24-MAY-2021 DEFINITION pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter. ACCESSION . VERSION . KEYWORDS pX330 Ctnnb1.1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 680) AUTHORS Xue W, Chen S, Yin H, Tammela T, Papagiannakopoulos T, Joshi NS, Cai W, Yang G, Bronson R, Crowley DG, Zhang F, Anderson DG, Sharp PA, Jacks T TITLE CRISPR-mediated direct mutation of cancer genes in the mouse liver. JOURNAL Nature. 2014 Aug 6. doi: 10.1038/nature13589. PUBMED 25119044 REFERENCE 2 (bases 1 to 680) AUTHORS . TITLE Direct Submission JOURNAL Exported May 24, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..680 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 123..363 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 123..143 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 294..313 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 393..468 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" ORIGIN 1 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 61 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 121 gtgagggcct atttcccatg attccttcat atttgcatat acgatacaag gctgttagag 181 agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta 241 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc 301 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag 361 gacgaaacac cgctgtggtg gtggcaccag aagttttaga gctagaaata gcaagttaaa 421 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgtttta 481 gagctagaaa tagcaagtta aaataaggct agtccgtttt tagcgcgtgc gccaattctg 541 cagacaaatg gctctagagg tacccgttac ataacttacg gtaaatggcc cgcctggctg 601 accgcccaac gacccccgcc cattgacgtc aatagtaacg ccaataggga ctttccattg 661 acgtcaatgg gtggagtatt //