LOCUS       Exported                 924 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse 
            beta-Catenin. Expresses Cas9 from CBh promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pX330 Ctnnb1.2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Xue W, Chen S, Yin H, Tammela T, Papagiannakopoulos T, Joshi NS, Cai
            W, Yang G, Bronson R, Crowley DG, Zhang F, Anderson DG, Sharp PA, 
            Jacks T
  TITLE     CRISPR-mediated direct mutation of cancer genes in the mouse liver.
  JOURNAL   Nature. 2014 Aug 6. doi: 10.1038/nature13589.
  PUBMED    25119044
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      54..63
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             63..128
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             135..155
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttaattacc tggagcacct gcctgaaatc actttttttc aggttggacc ggtgccacca
       61 tggactataa ggaccacgac ggagactaca aggatcatga tattgattac aaagacgatg
      121 acgataagat ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg
      181 acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg gccgtgatca
      241 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca
      301 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca
      361 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc
      421 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg
      481 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca
      541 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac
      601 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga
      661 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg
      721 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca
      781 acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc
      841 tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggaaacctga
      901 ttgccctgag cctgggcctg accc
//
