LOCUS Exported 722 bp ds-DNA linear SYN 24-MAY-2021 DEFINITION pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter. ACCESSION . VERSION . KEYWORDS pX330 Ctnnb1.2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 722) AUTHORS Xue W, Chen S, Yin H, Tammela T, Papagiannakopoulos T, Joshi NS, Cai W, Yang G, Bronson R, Crowley DG, Zhang F, Anderson DG, Sharp PA, Jacks T TITLE CRISPR-mediated direct mutation of cancer genes in the mouse liver. JOURNAL Nature. 2014 Aug 6. doi: 10.1038/nature13589. PUBMED 25119044 REFERENCE 2 (bases 1 to 722) AUTHORS . TITLE Direct Submission JOURNAL Exported May 24, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..722 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(10..57) /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" ORIGIN 1 gaattcttac tttttctttt ttgcctggcc ggcctttttc gtggccgccg gccttttgtc 61 gcctcccagc tgagacaggt cgatccgtgt ctcgtacagg ccggtgatgc tctggtggat 121 cagggtggcg tccagcacct ctttggtgct ggtgtacctc ttccggtcga tggtggtgtc 181 aaagtacttg aaggcggcag gggctcccag attggtcagg gtaaacaggt ggatgatatt 241 ctcggcctgc tctctgatgg gcttatcccg gtgcttgttg taggcggaca gcactttgtc 301 cagattagcg tcggccagga tcactctctt ggagaactcg ctgatctgct cgatgatctc 361 gtccaggtag tgcttgtgct gttccacaaa cagctgtttc tgctcattat cctcggggga 421 gcccttcagc ttctcatagt ggctggccag gtacaggaag ttcacatatt tggagggcag 481 ggccagttcg tttcccttct gcagttcgcc ggcagaggcc agcattctct tccggccgtt 541 ttccagctcg aacagggagt acttaggcag cttgatgatc aggtcctttt tcacttcttt 601 gtagcccttg gcttccagaa agtcgatggg attcttctcg aagctgcttc tttccatgat 661 ggtgatcccc agcagctctt tcacactctt cagtttcttg gacttgccct tttccacttt 721 gg //