LOCUS       Exported                 765 bp ds-DNA     linear   SYN 05-JUL-2021
DEFINITION  Mammalian expression of EGFP-TEV-S-tag PMFI for protein localization
            and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    pIC81
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 765)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 5, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             80..124
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gtccggaggg gatcggtgga gctccaccgg tggcggccgc tctagagaga atctttattt
       61 tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
      121 cagcggaggt ggaggtacta gtctcgagat ggccgaagca agtagcgcca atctaggcag
      181 cggctgtgag gaaaaaaggc atgaggggtc gtcttcggaa tctgtgccac ccggcactac
      241 catttcgagg gtgaagctcc tcgacaccat ggtggacact tttcttcaga agctggtcgc
      301 cgccggcagc taccagagat tcactgactg ctataagtgc ttctaccagt tgcagcctgc
      361 gatgacacag cgaatctatg acaagtttat agctcagttg cagacatcta tccgggagga
      421 aatctctgac atcaaagagg aggggaacct agaagctgtc ttgaatgcct tggataaaat
      481 tgtggaagaa ggcaaagtcc gcaaagagcc agcctggcgc cccagcggga tcccagagaa
      541 ggatctgcac agtgttatag caccctactt cctgcagcaa cgggacaccc tgcggcgcca
      601 tgtgcagaaa caggaggccg agaaccagca gctggcagat gccgtcctgg cagggcggag
      661 gcaggtggag gagctgcagc tacaggtcca ggcccagcag caggcctggc aggctctaca
      721 cagagaacag agggagctgg ttgctgtgct gagggagcct gagtg
//
