LOCUS       Exported                 972 bp ds-DNA     linear   SYN 30-MAY-2021
DEFINITION  C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5)..
ACCESSION   .
VERSION     .
KEYWORDS    PX854
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Zetsche B, Volz SE, Zhang F
  TITLE     A split-Cas9 architecture for inducible genome editing and 
            transcription modulation.
  JOURNAL   Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
  PUBMED    25643054
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 30, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        127..367
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     127..147
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     298..317
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        394..469
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        566..851
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
       61 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      121 acatgtgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
      181 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
      241 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
      301 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
      361 aaaggacgaa acaccgggtc ttcgagaaga cctgttttag agctagaaat agcaagttaa
      421 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgtttt
      481 agagctagaa atagcaagtt aaaataaggc tagtccgttt ttagcgcgtg cgccaattct
      541 gcagacaaat ggctctagag gtacccgtta cataacttac ggtaaatggc ccgcctggct
      601 gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg actttccatt
      661 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      721 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattgtg
      781 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      841 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
      901 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
      961 gggggggggg gg
//
