LOCUS       Exported                 812 bp ds-DNA     linear   SYN 14-JUN-2021
DEFINITION  TALEN array receiving vector with half-site NG RVD.
ACCESSION   .
VERSION     .
KEYWORDS    AI-CN443
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Martinez RA, Stein JL, Krostag AR, Nelson AM, Marken JS, Menon V, 
            May RC, Yao Z, Kaykas A, Geschwind DH, Grimley JS
  TITLE     Genome engineering of isogenic human ES cells to model autism 
            disorders.
  JOURNAL   Nucleic Acids Res. 2015 Mar 11. pii: gkv164.
  PUBMED    25765640
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 14, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(3..416)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWEDVFKGAPVSAL
                     RQACTASTAVTPPLFAIFSSCPVSSWSGGPLSSP"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     307..326
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        307..325
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(373..393)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(373..391)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(412..428)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(412..428)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(425..447)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    436..452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(460..490)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    505..526
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcgggtgagc ttagggggcc tccgctccag ctcgacaccg ggcagctgct gaagatcgcg
       61 aagagagggg gagtaacagc ggtagaggca gtgcacgcct ggcgcaatgc gctcaccggg
      121 gcccccttga acacgtcttc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
      301 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcctc
      361 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
      421 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      481 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgcga
      541 agacacctga cacccgaaca ggtggtcgcc attgcttcta atgggggagg acggccagcc
      601 ttggagtcca tcgtagccca attgtccagg cccgatcccg cgttggctgc gttaacgaat
      661 gaccatctgg tggcgttggc atgtcttggt ggacgacccg cgctcgatgc agtcaaaaag
      721 ggtctgcctc atgctcccgc attgatcaaa agaaccaacc ggcggattcc cgagagaact
      781 tcccatcgag tcgcgggatc ccaactagtc aa
//
