LOCUS       Exported               11824 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  harbours marker genes LEU2, URA3 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    pLUK
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11824)
  AUTHORS   Michael Mulleder, Kate Campbell, Olga Matsarskaia, Florian 
            Eckerstorfer, Markus Ralser
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities
  JOURNAL   F1000Research
REFERENCE   2  (bases 1 to 11824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11824
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        379..786
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             787..1881
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      complement(2444..2899)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2581..2602)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2793..2812
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3025..3047
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3039..3056
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3040..3056
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3063..3082
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3063..3081
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3090..3197
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(3209..3227)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3248..3264)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3248..3264)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3261..3283)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3272..3288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3296..3326)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3341..3362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3479..3496)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3650..4238)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3730..3749)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4409..5269)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5032..5051
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5270..5374)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5956..5974
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6012..6034)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6134..6153
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6417..6656
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             6657..10835
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     CDS             11022..1
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 aaaaactgta ttataagtaa atgcatgtat actaaactca caaattagag cttcaattta
       61 attatatcag ttattaccct atgcggtgtg aaataccgca cagatgcgta aggagaaaat
      121 accgcatcag gcgcgccatc gaggagaact tctagtatat ctacatacct aatattattg
      181 ccttattaaa aatggaatcc caacaattac atcaaaatcc acattctctt caaaatcaat
      241 tgtcctgtac ttccttgttc atgtgtgttc aaaaacgtta tatttatagg ataattatac
      301 tctatttctc aacaagtaat tggttgtttg gccgagcggt ctaaggcgcc tgattcaaga
      361 aatatcttga ccgcagttaa ctgtgggaat actcaggtat cgtaagatgc aagagttcga
      421 atctcttagc aaccattatt tttttcctca acataacgag aacacacagg ggcgctatcg
      481 cacagaatca aattcgatga ctggaaattt tttgttaatt tcagaggtcg cctgacgcat
      541 ataccttttt caactgaaaa attgggagaa aaaggaaagg tgagagcgcc ggaaccggct
      601 tttcatatag aatagagaag cgttcatgac taaatgcttg catcacaata cttgaagttg
      661 acaatattat ttaaggacct attgtttttt ccaataggtg gttagcaatc gtcttacttt
      721 ctaacttttc ttacctttta catttcagca atatatatat atatatttca aggatatacc
      781 attctaatgt ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt tggtcaagaa
      841 atcacagccg aagccattaa ggttcttaaa gctatttctg atgttcgttc caatgtcaag
      901 ttcgatttcg aaaatcattt aattggtggt gctgctatcg atgctacagg tgttccactt
      961 ccagatgagg cgctggaagc ctccaagaag gctgatgccg ttttgttagg tgctgtgggt
     1021 ggtcctaaat ggggtaccgg tagtgttaga cctgaacaag gtttactaaa aatccgtaaa
     1081 gaacttcaat tgtacgccaa cttaagacca tgtaactttg catccgactc tcttttagac
     1141 ttatctccaa tcaagccaca atttgctaaa ggtactgact tcgttgttgt cagagaatta
     1201 gtgggaggta tttactttgg taagagaaag gaagacgatg gtgatggtgt cgcttgggat
     1261 agtgaacaat acaccgttcc agaagtgcaa agaatcacaa gaatggccgc tttcatggcc
     1321 ctacaacatg agccaccatt gcctatttgg tccttggata aagctaatgt tttggcctct
     1381 tcaagattat ggagaaaaac tgtggaggaa accatcaaga acgaattccc tacattgaag
     1441 gttcaacatc aattgattga ttctgccgcc atgatcctag ttaagaaccc aacccaccta
     1501 aatggtatta taatcaccag caacatgttt ggtgatatca tctccgatga agcctccgtt
     1561 atcccaggtt ccttgggttt gttgccatct gcgtccttgg cctctttgcc agacaagaac
     1621 accgcatttg gtttgtacga accatgccac ggttctgctc cagatttgcc aaagaataag
     1681 gtcaacccta tcgccactat cttgtctgct gcaatgatgt tgaaattgtc attgaacttg
     1741 cctgaagaag gtaaggccat tgaagatgca gttaaaaagg ttttggatgc aggtatcaga
     1801 actggtgatt taggtggttc caacagtacc accgaagtcg gtgatgctgt cgccgaagaa
     1861 gttaagaaaa tccttgctta aaaagattct ctttttttat gatatttgta cataaacttt
     1921 ataaatgaaa ttcataatag aaacgacacg aaattacaaa atggaatatg ttcatagggt
     1981 agacgaaact atatacgcaa tctacataca tttatcaaga aggagaaaaa ggaggatgta
     2041 aaggaataca ggtaagcaaa ttgatactaa tggctcaacg tgataaggaa aaagaattgc
     2101 actttaacat taatattgac aaggaggagg gcaccacaca aaaagttagg tgtaacagaa
     2161 aatcatgaaa ctatgattcc taatttatat attggaggat tttctctaaa aaaaaaaaaa
     2221 tacaacaaat aaaaaacact caatgacctg accatttgat ggagtttaag tcaatacctt
     2281 cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa ttttactctg tcagaaacgg
     2341 ccttacgacg tagtcgagga tctcctaggt taattaagtt taaacagatc ctatgcggtg
     2401 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat
     2461 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     2521 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     2581 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     2641 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     2701 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     2761 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     2821 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     2881 gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     2941 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     3001 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     3061 tgtaatacga ctcactatag ggcgaattgg agctccaccg cggtggcggc cgctctagaa
     3121 ctagtgggtc ccccgggctg caggaattcg atatcaagct tatcgatacc gtcgacctgg
     3181 agggggggcc cggtaccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt
     3241 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3301 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3361 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     3421 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     3481 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3541 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3601 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3661 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3721 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3781 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3841 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3901 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3961 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4021 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4081 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4141 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4201 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4261 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4321 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4381 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4441 cagcgatctg tctatttcgt tcatccatag ttgcctgact gcccgtcgtg tagataacta
     4501 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4561 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4621 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4681 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     4741 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4801 catgatcccc catgttgtga aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4861 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4921 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4981 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5041 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5101 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5161 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5221 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5281 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5341 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
     5401 tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa tttgggaatt
     5461 tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta aataaagaag
     5521 gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa
     5581 aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag atatacattc
     5641 gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt ctacacagac
     5701 aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa aaggtagtat
     5761 ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta ttttttcttt
     5821 aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa tttaaattat
     5881 aattattttt atagcacgtg atgaaaagga cccccgtcct aagaaaccat tattatcatg
     5941 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     6001 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     6061 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg acgtcggggc
     6121 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaaccata attcaaaatg
     6181 acaccgatta tttaaagctg cagcatacga tatatataca tgtgtatata tgtataccta
     6241 tgaatgtcag taagtatgta tacgaacagt atgatactga agatgacaag gtaatgcatc
     6301 attctatacg tgtcattctg aacgaggcgc gctttccttt tttctttttg ctttttcttt
     6361 ttttttctct tgaactcgac gcgtcgagga aaactcttca atagttttgc cagcggaatt
     6421 ccacttgcaa ttacataaaa aattccggcg gtttttcgcg tgtgactcaa tgtcgaaata
     6481 cctgcctaat gaacatgaac atcgcccaaa tgtatttgaa gacccgctgg gagaagttca
     6541 agatatataa gtaacaagca gccaatagta taaaaaaaaa tctgagttta ttacctttcc
     6601 tggaatttca gtgaaaaact gctaattata gagagatatc acagagttac tcactaatga
     6661 ctaacgaaaa ggtctggata gagaagttgg ataatccaac tctttcagtg ttaccacatg
     6721 actttttacg cccacaacaa gaaccttata cgaaacaagc tacatattcg ttacagctac
     6781 ctcagctcga tgtgcctcat gatagttttt ctaacaaata cgctgtcgct ttgagtgtat
     6841 gggctgcatt gatatataga gtaaccggtg acgatgatat tgttctttat attgcgaata
     6901 acaaaatctt aagattcaat attcaaccaa cgtggtcatt taatgagctg tattctacaa
     6961 ttaacaatga gttgaacaag ctcaattcta ttgaggccaa tttttccttt gacgagctag
     7021 ctgaaaaaat tcaaagttgc caagatctgg aaaggacccc tcagttgttc cgtttggcct
     7081 ttttggaaaa ccaagatttc aaattagacg agttcaagca tcatttagtg gactttgctt
     7141 tgaatttgga taccagtaat aatgcgcatg ttttgaactt aatttataac agcttactgt
     7201 attcgaatga aagagtaacc attgttgcgg accaatttac tcaatatttg actgctgcgc
     7261 taagcgatcc atccaattgc ataactaaaa tctctctgat caccgcatca tccaaggata
     7321 gtttacctga tccaactaag aacttgggct ggtgcgattt cgtggggtgt attcacgaca
     7381 ttttccagga caatgctgaa gccttcccag agagaacctg tgttgtggag actccaacac
     7441 taaattccga caagtcccgt tctttcactt atcgcgacat caaccgcact tctaacatag
     7501 ttgcccatta tttgattaaa acaggtatca aaagaggtga tgtagtgatg atctattctt
     7561 ctaggggtgt ggatttgatg gtatgtgtga tgggtgtctt gaaagccggc gcaacctttt
     7621 cagttatcga ccctgcatat cccccagcca gacaaaccat ttacttaggt gttgctaaac
     7681 cacgtgggtt gattgttatt agagctgctg gacaattgga tcaactagta gaagattaca
     7741 tcaatgatga attggagatt gtttcaagaa tcaattccat cgctattcaa gaaaatggta
     7801 ccattgaagg tggcaaattg gacaatggcg aggatgtttt ggctccatat gatcactaca
     7861 aagacaccag aacaggtgtt gtagttggac cagattccaa cccaacccta tctttcacat
     7921 ctggttccga aggtattcct aagggtgttc ttggtagaca tttttccttg gcttattatt
     7981 tcaattggat gtccaaaagg ttcaacttaa cagaaaatga taaattcaca atgctgagcg
     8041 gtattgcaca tgatccaatt caaagagata tgtttacacc attattttta ggtgcccaat
     8101 tgtatgtccc tactcaagat gatattggta caccgggccg tttagcggaa tggatgagta
     8161 agtatggttg cacagttacc catttaacac ctgccatggg tcaattactt actgcccaag
     8221 ctactacacc attccctaag ttacatcatg cgttctttgt gggtgacatt ttaacaaaac
     8281 gtgattgtct gaggttacaa accttggcag aaaattgccg tattgttaat atgtacggta
     8341 ccactgaaac acagcgtgca gtttcttatt tcgaagttaa atcaaaaaat gacgatccaa
     8401 actttttgaa aaaattgaaa gatgtcatgc ctgctggtaa aggtatgttg aacgttcagc
     8461 tactagttgt taacaggaac gatcgtactc aaatatgtgg tattggcgaa ataggtgaga
     8521 tttatgttcg tgcaggtggt ttggccgaag gttatagagg attaccagaa ttgaataaag
     8581 aaaaatttgt gaacaactgg tttgttgaaa aagatcactg gaattatttg gataaggata
     8641 atggtgaacc ttggagacaa ttctggttag gtccaagaga tagattgtac agaacgggtg
     8701 atttaggtcg ttatctacca aacggtgact gtgaatgttg cggtagggct gatgatcaag
     8761 ttaaaattcg tgggttcaga atcgaattag gagaaataga tacgcacatt tcccaacatc
     8821 cattggtaag agaaaacatt actttagttc gcaaaaatgc cgacaatgag ccaacattga
     8881 tcacatttat ggtcccaaga tttgacaagc cagatgactt gtctaagttc caaagtgatg
     8941 ttccaaagga ggttgaaact gaccctatag ttaagggctt aatcggttac catcttttat
     9001 ccaaggacat caggactttc ttaaagaaaa gattggctag ctatgctatg ccttccttga
     9061 ttgtggttat ggataaacta ccattgaatc caaatggtaa agttgataag cctaaacttc
     9121 aattcccaac tcccaagcaa ttaaatttgg tagctgaaaa tacagtttct gaaactgacg
     9181 actctcagtt taccaatgtt gagcgcgagg ttagagactt atggttaagt atattaccta
     9241 ccaagccagc atctgtatca ccagatgatt cgtttttcga tttaggtggt cattctatct
     9301 tggctaccaa aatgattttt accttaaaga aaaagctgca agttgattta ccattgggca
     9361 caattttcaa gtatccaacg ataaaggcct ttgccgcgga aattgacaga attaaatcat
     9421 cgggtggatc atctcaaggt gaggtcgtcg aaaatgtcac tgcaaattat gcggaagacg
     9481 ccaagaaatt ggttgagacg ctaccaagtt cgtacccctc tcgagaatat tttgttgaac
     9541 ctaatagtgc cgaaggaaaa acaacaatta atgtgtttgt taccggtgtc acaggatttc
     9601 tgggctccta catccttgca gatttgttag gacgttctcc aaagaactac agtttcaaag
     9661 tgtttgccca cgtcagggcc aaggatgaag aagctgcatt tgcaagatta caaaaggcag
     9721 gtatcaccta tggtacttgg aacgaaaaat ttgcctcaaa tattaaagtt gtattaggcg
     9781 atttatctaa aagccaattt ggtctttcag atgagaagtg gatggatttg gcaaacacag
     9841 ttgatataat tatccataat ggtgcgttag ttcactgggt ttatccatat gccaaattga
     9901 gggatccaaa tgttatttca actatcaatg ttatgagctt agccgccgtc ggcaagccaa
     9961 agttctttga ctttgtttcc tccacttcta ctcttgacac tgaatactac tttaatttgt
    10021 cagataaact tgttagcgaa gggaagccag gcattttaga atcagacgat ttaatgaact
    10081 ctgcaagcgg gctcactggt ggatatggtc agtccaaatg ggctgctgag tacatcatta
    10141 gacgtgcagg tgaaaggggc ctacgtgggt gtattgtcag accaggttac gtaacaggtg
    10201 cctctgccaa tggttcttca aacacagatg atttcttatt gagatttttg aaaggttcag
    10261 tccaattagg taagattcca gatatcgaaa attccgtgaa tatggttcca gtagatcatg
    10321 ttgctcgtgt tgttgttgct acgtctttga atcctcccaa agaaaatgaa ttggccgttg
    10381 ctcaagtaac gggtcaccca agaatattat tcaaagacta cttgtatact ttacacgatt
    10441 atggttacga tgtcgaaatc gaaagctatt ctaaatggaa gaaatcattg gaggcgtctg
    10501 ttattgacag gaatgaagaa aatgcgttgt atcctttgct acacatggtc ttagacaact
    10561 tacctgaaag taccaaagct ccggaactag acgataggaa cgccgtggca tctttaaaga
    10621 aagacaccgc atggacaggt gttgattggt ctaatggaat aggtgttact ccagaagagg
    10681 ttggtatata tattgcattt ttaaacaagg ttggattttt acctccacca actcataatg
    10741 acaaacttcc actgccaagt atagaactaa ctcaagcgca aataagtcta gttgcttcag
    10801 gtgctggtgc tcgtggaagc tccgcagcag cttaaggttg agcattacgt atgatatgtg
    10861 catgcgattc ggtaatctcc gaacagaagg aagaacgaag gaaggagcac agacttagat
    10921 tggtatatat acgcatatgt agtgttgaag aaacatgaaa ttgcccagta ttcttaaccc
    10981 aactgcacag aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa gctacatata
    11041 aggaacgtgc tgctactcat cctagtcctg ttgctgccaa gctatttaat atcatgcacg
    11101 aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac caccaaggaa ttactggagt
    11161 tagttgaagc attaggtccc aaaatttgtt tactaaaaac acatgtggat atcttgactg
    11221 atttttccat ggagggcaca gttaagccgc taaaggcatt atccgccaag tacaattttt
    11281 tactcttcga agacagaaaa tttgctgaca ttggtaatac agtcaaattg cagtactctg
    11341 cgggtgtata cagaatagca gaatgggcag acattacgaa tgcacacggt gtggtgggcc
    11401 caggtattgt tagcggtttg aagcaggcgg cagaagaagt aacaaaggaa cctagaggcc
    11461 ttttgatgtt agcagaattg tcatgcaagg gctccctatc tactggagaa tatactaagg
    11521 gtactgttga cattgcgaag agcgacaaag attttgttat cggctttatt gctcaaagag
    11581 acatgggtgg aagagatgaa ggttacgatt ggttgattat gacacccggt gtgggtttag
    11641 atgacaaggg agacgcattg ggtcaacagt atagaaccgt ggatgatgtg gtctctacag
    11701 gatctgacat tattattgtt ggaagaggac tatttgcaaa gggaagggat gctaaggtag
    11761 agggtgaacg ttacagaaaa gcaggctggg aagcatattt gagaagatgc ggccagcaaa
    11821 acta
//
