LOCUS Exported 9242 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION harbours marker genes URA3 and LYS2. ACCESSION . VERSION . KEYWORDS pUK SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9242) AUTHORS Michael Mulleder, Kate Campbell, Olga Matsarskaia, Florian Eckerstorfer, Markus Ralser TITLE Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities JOURNAL F1000Research REFERENCE 2 (bases 1 to 9242) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9242 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(29..51) /label=pGEX 3' /note="pGEX vectors, reverse primer" promoter 164..403 /gene="S. cerevisiae LYS2" /label=LYS2 promoter CDS 404..4582 /codon_start=1 /gene="S. cerevisiae LYS2" /product="alpha-aminoadipate reductase, required for lysine biosynthesis" /label=LYS2 /note="yeast auxotrophic marker" /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA" CDS 4769..5572 /codon_start=1 /gene="S. cerevisiae URA3" /product="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /label=URA3 /note="yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" rep_origin complement(5703..6158) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5840..5861) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 6052..6071 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6284..6306 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 6298..6315 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 6299..6315 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 6322..6341 /label=T7 /note="T7 promoter, forward primer" promoter 6322..6340 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 6349..6456 /label=MCS /note="pBluescript multiple cloning site" promoter complement(6468..6486) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(6507..6523) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6507..6523) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6520..6542) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6531..6547 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6555..6585) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6600..6621 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6738..6755) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6909..7497) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6989..7008) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7668..8528) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8291..8310 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8529..8633) /gene="bla" /label=AmpR promoter primer_bind 9215..9233 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggcg tcgaggaaaa ctcttcaata gttttgccag cggaattcca cttgcaatta 181 cataaaaaat tccggcggtt tttcgcgtgt gactcaatgt cgaaatacct gcctaatgaa 241 catgaacatc gcccaaatgt atttgaagac ccgctgggag aagttcaaga tatataagta 301 acaagcagcc aatagtataa aaaaaaatct gagtttatta cctttcctgg aatttcagtg 361 aaaaactgct aattatagag agatatcaca gagttactca ctaatgacta acgaaaaggt 421 ctggatagag aagttggata atccaactct ttcagtgtta ccacatgact ttttacgccc 481 acaacaagaa ccttatacga aacaagctac atattcgtta cagctacctc agctcgatgt 541 gcctcatgat agtttttcta acaaatacgc tgtcgctttg agtgtatggg ctgcattgat 601 atatagagta accggtgacg atgatattgt tctttatatt gcgaataaca aaatcttaag 661 attcaatatt caaccaacgt ggtcatttaa tgagctgtat tctacaatta acaatgagtt 721 gaacaagctc aattctattg aggccaattt ttcctttgac gagctagctg aaaaaattca 781 aagttgccaa gatctggaaa ggacccctca gttgttccgt ttggcctttt tggaaaacca 841 agatttcaaa ttagacgagt tcaagcatca tttagtggac tttgctttga atttggatac 901 cagtaataat gcgcatgttt tgaacttaat ttataacagc ttactgtatt cgaatgaaag 961 agtaaccatt gttgcggacc aatttactca atatttgact gctgcgctaa gcgatccatc 1021 caattgcata actaaaatct ctctgatcac cgcatcatcc aaggatagtt tacctgatcc 1081 aactaagaac ttgggctggt gcgatttcgt ggggtgtatt cacgacattt tccaggacaa 1141 tgctgaagcc ttcccagaga gaacctgtgt tgtggagact ccaacactaa attccgacaa 1201 gtcccgttct ttcacttatc gcgacatcaa ccgcacttct aacatagttg cccattattt 1261 gattaaaaca ggtatcaaaa gaggtgatgt agtgatgatc tattcttcta ggggtgtgga 1321 tttgatggta tgtgtgatgg gtgtcttgaa agccggcgca accttttcag ttatcgaccc 1381 tgcatatccc ccagccagac aaaccattta cttaggtgtt gctaaaccac gtgggttgat 1441 tgttattaga gctgctggac aattggatca actagtagaa gattacatca atgatgaatt 1501 ggagattgtt tcaagaatca attccatcgc tattcaagaa aatggtacca ttgaaggtgg 1561 caaattggac aatggcgagg atgttttggc tccatatgat cactacaaag acaccagaac 1621 aggtgttgta gttggaccag attccaaccc aaccctatct ttcacatctg gttccgaagg 1681 tattcctaag ggtgttcttg gtagacattt ttccttggct tattatttca attggatgtc 1741 caaaaggttc aacttaacag aaaatgataa attcacaatg ctgagcggta ttgcacatga 1801 tccaattcaa agagatatgt ttacaccatt atttttaggt gcccaattgt atgtccctac 1861 tcaagatgat attggtacac cgggccgttt agcggaatgg atgagtaagt atggttgcac 1921 agttacccat ttaacacctg ccatgggtca attacttact gcccaagcta ctacaccatt 1981 ccctaagtta catcatgcgt tctttgtggg tgacatttta acaaaacgtg attgtctgag 2041 gttacaaacc ttggcagaaa attgccgtat tgttaatatg tacggtacca ctgaaacaca 2101 gcgtgcagtt tcttatttcg aagttaaatc aaaaaatgac gatccaaact ttttgaaaaa 2161 attgaaagat gtcatgcctg ctggtaaagg tatgttgaac gttcagctac tagttgttaa 2221 caggaacgat cgtactcaaa tatgtggtat tggcgaaata ggtgagattt atgttcgtgc 2281 aggtggtttg gccgaaggtt atagaggatt accagaattg aataaagaaa aatttgtgaa 2341 caactggttt gttgaaaaag atcactggaa ttatttggat aaggataatg gtgaaccttg 2401 gagacaattc tggttaggtc caagagatag attgtacaga acgggtgatt taggtcgtta 2461 tctaccaaac ggtgactgtg aatgttgcgg tagggctgat gatcaagtta aaattcgtgg 2521 gttcagaatc gaattaggag aaatagatac gcacatttcc caacatccat tggtaagaga 2581 aaacattact ttagttcgca aaaatgccga caatgagcca acattgatca catttatggt 2641 cccaagattt gacaagccag atgacttgtc taagttccaa agtgatgttc caaaggaggt 2701 tgaaactgac cctatagtta agggcttaat cggttaccat cttttatcca aggacatcag 2761 gactttctta aagaaaagat tggctagcta tgctatgcct tccttgattg tggttatgga 2821 taaactacca ttgaatccaa atggtaaagt tgataagcct aaacttcaat tcccaactcc 2881 caagcaatta aatttggtag ctgaaaatac agtttctgaa actgacgact ctcagtttac 2941 caatgttgag cgcgaggtta gagacttatg gttaagtata ttacctacca agccagcatc 3001 tgtatcacca gatgattcgt ttttcgattt aggtggtcat tctatcttgg ctaccaaaat 3061 gatttttacc ttaaagaaaa agctgcaagt tgatttacca ttgggcacaa ttttcaagta 3121 tccaacgata aaggcctttg ccgcggaaat tgacagaatt aaatcatcgg gtggatcatc 3181 tcaaggtgag gtcgtcgaaa atgtcactgc aaattatgcg gaagacgcca agaaattggt 3241 tgagacgcta ccaagttcgt acccctctcg agaatatttt gttgaaccta atagtgccga 3301 aggaaaaaca acaattaatg tgtttgttac cggtgtcaca ggatttctgg gctcctacat 3361 ccttgcagat ttgttaggac gttctccaaa gaactacagt ttcaaagtgt ttgcccacgt 3421 cagggccaag gatgaagaag ctgcatttgc aagattacaa aaggcaggta tcacctatgg 3481 tacttggaac gaaaaatttg cctcaaatat taaagttgta ttaggcgatt tatctaaaag 3541 ccaatttggt ctttcagatg agaagtggat ggatttggca aacacagttg atataattat 3601 ccataatggt gcgttagttc actgggttta tccatatgcc aaattgaggg atccaaatgt 3661 tatttcaact atcaatgtta tgagcttagc cgccgtcggc aagccaaagt tctttgactt 3721 tgtttcctcc acttctactc ttgacactga atactacttt aatttgtcag ataaacttgt 3781 tagcgaaggg aagccaggca ttttagaatc agacgattta atgaactctg caagcgggct 3841 cactggtgga tatggtcagt ccaaatgggc tgctgagtac atcattagac gtgcaggtga 3901 aaggggccta cgtgggtgta ttgtcagacc aggttacgta acaggtgcct ctgccaatgg 3961 ttcttcaaac acagatgatt tcttattgag atttttgaaa ggttcagtcc aattaggtaa 4021 gattccagat atcgaaaatt ccgtgaatat ggttccagta gatcatgttg ctcgtgttgt 4081 tgttgctacg tctttgaatc ctcccaaaga aaatgaattg gccgttgctc aagtaacggg 4141 tcacccaaga atattattca aagactactt gtatacttta cacgattatg gttacgatgt 4201 cgaaatcgaa agctattcta aatggaagaa atcattggag gcgtctgtta ttgacaggaa 4261 tgaagaaaat gcgttgtatc ctttgctaca catggtctta gacaacttac ctgaaagtac 4321 caaagctccg gaactagacg ataggaacgc cgtggcatct ttaaagaaag acaccgcatg 4381 gacaggtgtt gattggtcta atggaatagg tgttactcca gaagaggttg gtatatatat 4441 tgcattttta aacaaggttg gatttttacc tccaccaact cataatgaca aacttccact 4501 gccaagtata gaactaactc aagcgcaaat aagtctagtt gcttcaggtg ctggtgctcg 4561 tggaagctcc gcagcagctt aaggttgagc attacgtatg atatgtgcat gcgattcggt 4621 aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg tatatatacg 4681 catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac tgcacagaac 4741 aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg aacgtgctgc 4801 tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa agcaaacaaa 4861 cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag ttgaagcatt 4921 aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt tttccatgga 4981 gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac tcttcgaaga 5041 cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg gtgtatacag 5101 aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag gtattgttag 5161 cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt tgatgttagc 5221 agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta ctgttgacat 5281 tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca tgggtggaag 5341 agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg acaagggaga 5401 cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat ctgacattat 5461 tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta 5521 cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact aaaaaactgt 5581 attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt aattatatca 5641 gttattaccc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 5701 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 5761 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 5821 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 5881 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 5941 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 6001 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 6061 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 6121 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc 6181 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg 6241 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt 6301 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattgga gctccaccgc 6361 ggtggcggcc gctctagaac tagtgggtcc cccgggctgc aggaattcga tatcaagctt 6421 atcgataccg tcgacctgga gggggggccc ggtaccagct tttgttccct ttagtgaggg 6481 ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 6541 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 6601 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 6661 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 6721 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 6781 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 6841 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 6901 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 6961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 7021 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 7081 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 7141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 7201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 7261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 7321 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 7381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 7441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 7501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 7561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 7621 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 7681 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactg 7741 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 7801 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 7861 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 7921 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 7981 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 8041 caacgatcaa ggcgagttac atgatccccc atgttgtgaa aaaaagcggt tagctccttc 8101 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 8161 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 8221 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 8281 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 8341 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 8401 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 8461 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 8521 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 8581 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 8641 ccccgaaaag tgccacctat cgcttgcctg taacttacac gcgcctcgta tcttttaatg 8701 atggaataat ttgggaattt actctgtgtt tatttatttt tatgttttgt atttggattt 8761 tagaaagtaa ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa taaacaaagg 8821 tttaaaaaat ttcaacaaaa agcgtacttt acatatatat ttattagaca agaaaagcag 8881 attaaataga tatacattcg attaacgata agtaaaatgt aaaatcacag gattttcgtg 8941 tgtggtcttc tacacagaca agatgaaaca attcggcatt aatacctgag agcaggaaga 9001 gcaagataaa aggtagtatt tgttggcgat ccccctagag tcttttacat cttcggaaaa 9061 caaaaactat tttttcttta atttcttttt ttactttcta tttttaattt atatatttat 9121 attaaaaaat ttaaattata attattttta tagcacgtga tgaaaaggac ccccgtccta 9181 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 9241 tc //