LOCUS Exported 2763 bp ds-DNA linear SYN 01-JUN-2021 DEFINITION mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration. ACCESSION . VERSION . KEYWORDS Cry2 mCherry RGS4(del 1-33) in pcDNA3.1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2763) AUTHORS O'Neill PR, Gautam N TITLE Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration. JOURNAL Mol Biol Cell. 2014 Aug 1;25(15):2305-14. doi: 10.1091/mbc.E14-04-0870. Epub 2014 Jun 11. PUBMED 24920824 REFERENCE 2 (bases 1 to 2763) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 1, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2763 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..1494 /codon_start=1 /product="N-terminal photolyase homology region of Arabidopsis thaliana cryptochrome 2" /label=CRY2PHR /note="binds to CIB1 upon exposure to blue light (Kennedy et al., 2010)" /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA" regulatory 1513..1522 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1519..2226 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(1664..1682) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1932..1951 /label=mCherry-F /note="mCherry, forward primer" ORIGIN 1 atgaagatgg acaaaaagac tatagtttgg tttagaagag acctaaggat tgaggataat 61 cctgcattag cagcagctgc tcacgaagga tctgtttttc ctgtcttcat ttggtgtcct 121 gaagaagaag gacagtttta tcctggaaga gcttcaagat ggtggatgaa acaatcactt 181 gctcacttat ctcaatcctt gaaggctctt ggatctgacc tcactttaat caaaacccac 241 aacacgattt cagcgatctt ggattgtatc cgcgttaccg gtgctacaaa agtcgtcttt 301 aaccacctct atgatcctgt ttcgttagtt cgggaccata ccgtaaagga gaagctggtg 361 gaacgtggga tctctgtgca aagctacaat ggagatctat tgtatgaacc gtgggagata 421 tactgcgaaa agggcaaacc ttttacgagt ttcaattctt actggaagaa atgcttagat 481 atgtcgattg aatccgttat gcttcctcct ccttggcggt tgatgccaat aactgcagcg 541 gctgaagcga tttgggcgtg ttcgattgaa gaactagggc tggagaatga ggccgagaaa 601 ccgagcaatg cgttgttaac tagagcttgg tctccaggat ggagcaatgc tgataagtta 661 ctaaatgagt tcatcgagaa gcagttgata gattatgcaa agaacagcaa gaaagttgtt 721 gggaattcta cttcactact ttctccgtat ctccatttcg gggaaataag cgtcagacac 781 gttttccagt gtgcccggat gaaacaaatt atatgggcaa gagataagaa cagtgaagga 841 gaagaaagtg cagatctttt tcttagggga atcggtttaa gagagtattc tcggtatata 901 tgtttcaact tcccgtttac tcacgagcaa tcgttgttga gtcatcttcg gtttttccct 961 tgggatgctg atgttgataa gttcaaggcc tggagacaag gcaggaccgg ttatccgttg 1021 gtggatgccg gaatgagaga gctttgggct accggatgga tgcataacag aataagagtg 1081 attgtttcaa gctttgctgt gaagtttctt ctccttccat ggaaatgggg aatgaagtat 1141 ttctgggata cacttttgga tgctgatttg gaatgtgaca tccttggctg gcagtatatc 1201 tctgggagta tccccgatgg ccacgagctt gatcgcttgg acaatcccgc gttacaaggc 1261 gccaaatatg acccagaagg tgagtacata aggcaatggc ttcccgagct tgcgagattg 1321 ccaactgaat ggatccatca tccatgggac gctcctttaa ccgtactcaa agcttctggt 1381 gtggaactcg gaacaaacta tgcgaaaccc attgtagaca tcgacacagc tcgtgagcta 1441 ctagctaaag ctatttcaag aacccgtgaa gcacagatca tgatcggagc agcagcccgg 1501 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag 1561 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc 1621 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc 1681 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc 1741 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag 1801 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag 1861 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc 1921 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg 1981 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac 2041 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg 2101 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc 2161 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg 2221 tacaagggta ccatggaaca tagttcttca cacagcaaga aggacaaagt agtaacttgc 2281 cagagggtga gccaagaaga agtcaagaaa tgggctgaat cgctggaaaa cctgattaac 2341 catgaatgtg gactggcagc tttcaaagct ttcctgaagt cagaatacag tgaggagaac 2401 attgacttct ggatcagctg tgaggagtac aagaaaatca aatcaccttc taaactaagt 2461 cccaaggcca agaagatcta caatgagttc atctctgtgc aggcaacaaa agaggtgaac 2521 ctggattctt gcaccagaga ggagacaagc cggaacatgt tagagcccac gataacctgt 2581 tttgatgaag cccagaagaa gattttcaac ctgatggaaa aggattcata ccgtcgtttc 2641 ctcaagtctc gattctatct tgacctgacc aatccttcca gctgcggggc agagaagcag 2701 aaaggagcca agagttctgc agactgtgct tccctggtcc ctcagtgtgc cttgtagtct 2761 aga //