LOCUS       Exported               10333 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral, doxycycline-inducible system to express transgene of 
            interest and EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    pTet-IRES-EGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10333)
  AUTHORS   Shuen WH, Kan R, Yu Z, Lung HL, Lung ML
  TITLE     Novel lentiviral-inducible transgene expression systems and 
            versatile single-plasmid reporters for in vitro and in vivo cancer 
            biology studies.
  JOURNAL   Cancer Gene Ther. 2015 Feb 27. doi: 10.1038/cgt.2015.9.
  PUBMED    25721206
REFERENCE   2  (bases 1 to 10333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10333
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2067..2337
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2370..2408
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2405..2429
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    2600..2717
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2784..3335
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2928..2945)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3155..3174
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3338..4054
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3338..4054
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3383..3404)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3644..3663)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3991..4012
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4133..4149)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4134..4150)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    4163..4751
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4216..4236)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4634..4645)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    4895..4928
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     LTR             4948..5128
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5153..5171)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5154..5171)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5461..5480)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5694..5713)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5813..5835
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5873..5891)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5959..6063
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6064..6924
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6282..6301)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7095..7683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7584..7603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7837..7854
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7924..7944)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7929..8258
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8109..8244
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8171..8190
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          9392..9457
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9587..9607
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10032..10166
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10069..10088)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10123..10142
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgcta gagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2101 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2161 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2221 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2281 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     2341 gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt acggtgggag gcctatataa
     2401 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2461 tccatagaag acaccgggac cgatccagcc tccgcggccc cgaattgtga gatctcaacc
     2521 ggtttaggcg gccgcttctg ctagctaatt cgagctcggt acccggggat cctctagaat
     2581 ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg
     2641 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     2701 caaaaattca aaattttatc gatcacgaga ctagcctcga ggtttaaact acgggctgca
     2761 ggaattccgc cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     2821 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     2881 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     2941 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     3001 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     3061 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     3121 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     3181 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     3241 cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg
     3301 gacgtggttt tcctttgaaa aacacgatga taataccatg gtgagcaagg gcgaggagct
     3361 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3421 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     3481 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     3541 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3601 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     3661 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3721 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     3781 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     3841 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     3901 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     3961 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4021 cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc gactagctag
     4081 tagctagcta gctagtcgag ctcaagcttc gaattcgata tcaagcttat cgcgataccg
     4141 tcgacctcga gggaattccg ataatcaacc tctggattac aaaatttgtg aaagattgac
     4201 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     4261 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     4321 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4381 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     4441 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     4501 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     4561 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     4621 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4681 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     4741 cgcctccccg catcgggaat tcgagctcgg tacctttaag accaatgact tacaaggcag
     4801 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     4861 aacgaagaca agatgggatc aattcaccat gggaataact tcgtatagca tacattatac
     4921 gaagttatgc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     4981 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5041 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5101 ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt
     5161 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
     5221 acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
     5281 ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
     5341 acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga
     5401 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
     5461 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
     5521 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
     5581 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
     5641 gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc
     5701 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     5761 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     5821 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     5881 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     5941 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6001 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6061 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6121 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6181 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6241 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6301 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6361 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6421 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6481 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6541 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6601 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6661 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6721 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6781 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6841 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6901 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6961 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7021 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7081 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7141 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7201 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     7261 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7321 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7381 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7441 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
     7501 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7561 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7621 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7681 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7741 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7801 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7861 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     7921 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     7981 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     8041 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     8101 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8161 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8221 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct
     8281 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
     8341 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
     8401 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
     8461 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
     8521 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac
     8581 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     8641 gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg
     8701 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     8761 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     8821 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     8881 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     8941 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     9001 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     9061 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     9121 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     9181 cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc
     9241 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt
     9301 tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     9361 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     9421 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     9481 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     9541 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     9601 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     9661 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     9721 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
     9781 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
     9841 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     9901 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     9961 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
    10021 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    10081 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    10141 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    10201 aacttcccac cccataccct attaccactg ccaattacct agtggtttca tttactctaa
    10261 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    10321 acaaacttag tag
//
