LOCUS       Exported                6659 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses tdTomato under the UbC promoter. This promoter expresses 
            transgenes in neurons at higher levels than plasmids with the CMV. 
            Optimal for tracing axons in tissue clearing procedures.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-UbC-tdTomato
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Soderblom C, Lee DH, Dawood A, Carballosa M, Jimena Santamaria A, 
            Benavides FD, Jergova S, Grumbles RM, Thomas CK, Park KK, Guest JD, 
            Lemmon VP, Lee JK, Tsoulfas P
  TITLE     3D Imaging of Axons in Transparent Spinal Cords from Rodents and 
            Nonhuman Primates
  JOURNAL   Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/ENEURO.0001-15.2015.
  PUBMED    26023683
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        150..549
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1119..1138
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     1883..1902
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1925..1934
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    3406..3882
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3605..3624)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3922..4062
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3922..4051
                     /label=AAV2 ITR
     rep_origin      4137..4592
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4224..4243)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4434..4455
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4609..4628)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4728..4750
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4788..4806)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4874..4978
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4979..5839
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5197..5216)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6010..6598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6499..6518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgcg gcctccgcgc cgggttttgg cgcctcccgc
      181 gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc
      241 tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga
      301 accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt
      361 tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga
      421 gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca
      481 gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg
      541 tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc
      601 ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg
      661 ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga
      721 atagaacctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
      781 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg
      841 gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc
      901 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc
      961 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc
     1021 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt
     1081 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat
     1141 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcacctttga aatgtaatca
     1201 tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg
     1261 tttttggctt ttttgttaga cgaagctaag gcgcgcctga gaacttcagg gtgagtttgg
     1321 ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat ggagggggca
     1381 aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat gcatggaccc
     1441 tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt
     1501 ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta acgaattttt
     1561 aaattcactt ttgtttattt gtcagattgt aagatcccat cgattctaga caatcagggt
     1621 atattatatt gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt
     1681 agaatatttc tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac
     1741 tacatcctgg tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag
     1801 atgaggataa aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc
     1861 ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcaagcttgg
     1921 atccgccacc atggtgtcca agggcgagga agtgatcaaa gaattcatgc ggttcaaagt
     1981 gcggatggaa ggcagcatga acggccacga gttcgagatc gagggcgagg gggagggcag
     2041 accctacgag ggcacacaga ccgccaagct gaaagtgaca aagggcggac ccctgccctt
     2101 cgcctgggac atcctgagcc cccagtttat gtacggcagc aaggcctacg tgaagcaccc
     2161 cgccgacatc cccgactaca agaagctgag cttccccgag ggcttcaagt gggagagagt
     2221 gatgaacttc gaggacggcg gcctggtgac agtgacccag gacagcagcc tgcaggacgg
     2281 caccctgatc tacaaagtga agatgcgggg caccaacttc ccccccgacg gccccgtgat
     2341 gcagaaaaag accatgggct gggaggccag caccgagcgg ctgtacccta gggatggcgt
     2401 gctgaagggc gagatccacc aggccctgaa gctgaaggat ggcggccact acctggtgga
     2461 attcaagacc atctacatgg ccaagaaacc cgtgcagctg cctggctact actacgtgga
     2521 caccaagctg gacatcacca gccacaacga ggactacacc atcgtggaac agtacgagcg
     2581 gagcgagggc cggcaccacc tgtttctggg ccacggcaca ggcagcaccg gcagcggaag
     2641 ctctggcacc gccagcagcg aggacaacaa catggccgtg atcaaagaat tcatgcgctt
     2701 taaagtgcgc atggaaggct ctatgaatgg gcacgagttt gaaattgagg gggaaggcga
     2761 gggacggccc tatgagggaa cccagacagc taaactgaaa gtgacaaaag ggggccctct
     2821 gccttttgcc tgggatattc tgtctcctca gtttatgtat gggtctaagg cctatgtgaa
     2881 acatcctgcc gatattccag actataagaa actgtccttc ccagaggggt ttaaatggga
     2941 acgcgtgatg aattttgagg atgggggact ggtgaccgtg acacaggata gttctctgca
     3001 ggatgggaca ctgatctaca aagtgaaaat gagagggacc aattttcccc ccgatgggcc
     3061 tgtgatgcag aaaaaaacaa tgggatggga agcctctacc gagagactgt acccccgcga
     3121 cggggtgctg aaaggggaaa ttcatcaggc tctgaaactg aaagacgggg ggcattatct
     3181 ggtggaattc aaaacaatct atatggctaa aaagcctgtg cagctgcctg ggtactatta
     3241 tgtggataca aaactggata tcacctccca caatgaggat tatacaattg tggaacagta
     3301 tgagagatcc gaggggcgcc atcatctgtt tctgtacggc atggacgagc tgtacaagtg
     3361 aagatctctc gagtctagag ttaacggcca tattggccaa tctacgggtg gcatccctgt
     3421 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     3481 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     3541 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     3601 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     3661 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     3721 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     3781 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     3841 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc
     3901 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     3961 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     4021 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
     4081 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
     4141 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4201 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4261 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4321 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     4381 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4441 cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg
     4501 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4561 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
     4621 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     4681 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     4741 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     4801 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     4861 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4921 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4981 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5041 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5101 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5161 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5221 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5281 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5341 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5401 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5461 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5521 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5581 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5641 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5701 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5761 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5821 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5881 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5941 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6001 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6061 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6121 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     6181 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6241 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6301 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6361 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6421 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6481 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6541 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6601 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
