LOCUS Exported 5863 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures. . ACCESSION . VERSION . KEYWORDS pAAV-UbC-eGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5863) AUTHORS Soderblom C, Lee DH, Dawood A, Carballosa M, Jimena Santamaria A, Benavides FD, Jergova S, Grumbles RM, Thomas CK, Park KK, Guest JD, Lemmon VP, Lee JK, Tsoulfas P TITLE 3D Imaging of Axons in Transparent Spinal Cords from Rodents and Nonhuman Primates JOURNAL Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/ENEURO.0001-15.2015. PUBMED 26023683 REFERENCE 2 (bases 1 to 5863) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5863 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..141 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 12..141 /label=AAV2 ITR promoter 150..549 /label=UbC promoter /note="human ubiquitin C promoter" primer_bind 1119..1138 /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" primer_bind 1835..1854 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" CDS 1882..2598 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1927..1948) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2188..2207) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2535..2556 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 2610..3086 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(2809..2828) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3126..3266 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3126..3255 /label=AAV2 ITR rep_origin 3341..3796 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3428..3447) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3638..3659 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3813..3832) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3932..3954 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(3992..4010) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4078..4182 /gene="bla" /label=AmpR promoter CDS 4183..5043 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4401..4420) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5214..5802 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5703..5722 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgcg gcctccgcgc cgggttttgg cgcctcccgc 181 gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc 241 tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga 301 accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt 361 tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga 421 gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca 481 gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg 541 tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc 601 ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg 661 ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga 721 atagaacctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc 781 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg 841 gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc 901 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc 961 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc 1021 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt 1081 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat 1141 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc 1201 atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc 1261 gtttttggct tttttgttag acgaagctaa ggcgcgcctg agaacttcag ggtgagtttg 1321 gggacccttg attgttcttt ctttttcgct attgtaaaat tcatgttata tggagggggc 1381 aaagttttca gggtgttgtt tagaatggga agatgtccct tgtatcacca tgcatggacc 1441 ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt ctcctcttat 1501 tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt aacgaatcta 1561 gacaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat tgttataatt 1621 aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt 1681 ggtagaaaca actacatcct ggtcatcatc ctgcctttct ctttatggtt acaatgatat 1741 acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat 1801 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct 1861 catcaagctt aaggatccac catggtgagc aagggcgagg agctgttcac cggggtggtg 1921 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 1981 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 2041 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc 2101 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 2161 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 2221 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 2281 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 2341 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 2401 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 2461 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ctctgagcaa agaccccaac 2521 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 2581 atggacgagc tgtacaagtg aagatctacg ggtggcatcc ctgtgacccc tccccagtgc 2641 ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa 2701 gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg 2761 tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac 2821 caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag 2881 cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca 2941 gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca 3001 actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt 3061 gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg 3121 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac 3181 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag 3241 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct 3301 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca 3361 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 3421 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 3481 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 3541 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt 3601 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 3661 acaacactca accctatctc gggctattct tttgatttat aagggatttt gccgatttcg 3721 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata 3781 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta 3841 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg 3901 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca 3961 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt 4021 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc 4081 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 4141 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 4201 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 4261 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 4321 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 4381 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 4441 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 4501 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 4561 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 4621 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 4681 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 4741 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 4801 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 4861 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 4921 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 4981 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 5041 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 5101 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 5161 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 5221 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 5281 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 5341 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 5401 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 5461 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 5521 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 5581 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 5641 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 5701 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 5761 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 5821 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt //