LOCUS       Exported                 361 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 
            (aa 2-2414) driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA-dCas9-FLp300
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             68..133
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             140..160
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
       61 cgccatggac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga
      121 tgacgatgac aagatggccc ccaagaagaa gaggaaggtg ggccgcggaa tggataagaa
      181 atactcaata ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga tcactgatga
      241 atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa
      301 aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct
      361 c
//
