LOCUS       Exported                 710 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 
            (aa 2-2414) driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA-dCas9-FLp300
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(4..30)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agaagcgtag tccggaacgt cgtacgggta gtgtatgtct agtgtactct gtgagaggtt
       61 tgaattcaag tctgagttat cggtgctgag tcccaggtcc gtggcgcttg caccatggag
      121 gtttgccatg cctggattgc tagcaagctg agaaagcatt gaattctggt ccgggctggc
      181 aaaatgccct tgttccatgg ggttggcctg ggcagctacc agtccaggat gtggggaact
      241 tgtctgtggg gaaacgtggt gtggagaagg ctgaggctgc atccttgggg aaggactgga
      301 gtgggggggc tgggactgtg gccgtggaga agggacaggc tggggagagc gcacttgatt
      361 ggagagagaa ttagggatct gctggccttg taggtgtggg gactgggcct gatttgggag
      421 catatgctgc tgggggctca tggggttggg ctgaacaggg gaccccatct gttgctgaag
      481 gagtcgctgc tgataggcct gtagactggc acctgcctct gctcccaagg cctggggaag
      541 ctggcctatc tgtcccatat ttccttgttg catctgttgc atgtgatgct gcatccgctg
      601 ctgctgctgt ggtgggtagc caactccttg gggttgctgg aactggttat ggttggccat
      661 tccagggcct attcctggcc ctgctccctg ttgctgctgc tgttgcatca
//
