LOCUS Exported 388 bp ds-DNA linear SYN 30-MAY-2021 DEFINITION leu2 deletion gRNA cassette carried by pRS42H. ACCESSION . VERSION . KEYWORDS gRNA-leu-HYB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 388) AUTHORS Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS TITLE Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease. JOURNAL Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3. PUBMED 25281382 REFERENCE 2 (bases 1 to 388) AUTHORS . TITLE Direct Submission JOURNAL Exported May 30, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..388 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..269 /label=SNR52 promoter /note="promoter for the S. cerevisiae small nucleolar RNA gene SNR52" misc_RNA 290..364 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" terminator 369..388 /label=SUP4 terminator /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" ORIGIN 1 tctttgaaaa gataatgtat gattatgctt tcactcatat ttatacagaa acttgatgtt 61 ttctttcgag tatatacaag gtgattacat gtacgtttga agtacaactc tagattttgt 121 agtgccctct tgggctagcg gtaaaggtgc gcattttttc acaccctaca atgttctgtt 181 caaaagattt tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt cggcgttcga 241 aacttctccg cagtgaaaga taaatgatca atcacagccg aagccattag ttttagagct 301 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc 361 ggtggtgctt tttttgtttt ttatgtct //